Growth requirements ofHaemophilus vaginalis

Dunkelberg, W. E.; McVeigh, Ilda

doi:10.1007/bf02219124

Cited by 36 publications

(27 citation statements)

References 14 publications

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“…Concentrations of sorbitan mono-oleate < 0.001% in BHI failed to support the growth of lactobacilli. As interaction between the micro-organisms is probably important in the pathogenesis of bacterial vaginosis, we are currently reviewing the semi-defined medium developed for G. vaginalis in 1961 [14] with a view to developing a defined medium to grow both organisms in this continuous culture biofilm system. The fact that the system can be used to study the growth of the bacteria in steady state enabled a preliminary assessment of the haemolytic activity of G. vaginalis.…”

Section: Discussionmentioning

confidence: 99%

The growth of Gardnerella vaginalis and Lactobacillus acidophilus in Sorbarod biofilms

Muli

Struthers²

1998

Journal of Medical Microbiology

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Sorbarod biofilms were investigated for their suitability in establishing continuous culture biofilms for the study of bacterial vaginosis. Two important organisms in the condition, Gardnerella vaginalis and Lactobacillus acidophilus, were studied. In contrast to growth in broth culture, both organisms were maintained for at least 96 h in a steady state on the biofilms. With G. vaginalis, the haemolytic activity was consistently maintained in the biofilms in contrast to short-term activity in broth culture which matched the bacterial titre. The simple Sorbarod system appears to be suitable for studying the growth conditions of bacteria in continuous culture and has potential for investigating interactions between micro-organisms.

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Section: Discussionmentioning

confidence: 99%

The growth of Gardnerella vaginalis and Lactobacillus acidophilus in Sorbarod biofilms

Muli

Struthers²

1998

Journal of Medical Microbiology

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“…Stock cultures were stored at À708C in de Man, Rogosa, Strains of G. vaginalis were isolated from vaginal swab samples from women with BV [19]. Stock cultures were stored at À708C in peptone-dextrose-starch broth (PDS broth) [20] supplemented with glycerol 20%. Strains were cultured on PDS agar at 378C for 48 h in an anaerobic atmosphere.…”

Section: Bacterial Isolatesmentioning

confidence: 99%

Characterisation and selection of a Lactobacillus species to re-colonise the vagina of women with recurrent bacterial vaginosis

McLEAN

Rosenstein²

2000

Journal of Medical Microbiology

132

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“…However, a phenotypic difference is seen in the nutritional requirement for adenine displayed by strain 594 (ATCC 14018 ) but not by strain 317 (ATCC 14019) (55). The existence of adenine auxotrophy and other possibly different natural requirements suggests that a system of typing could be developed for G. vaginalis on the basis of multiple nutritional requirements detected by use of chemically defined media, analogous to auxotyping of gonococci (34 (106).…”

Section: Susceptibility To Chemotherapeutic Agentsmentioning

confidence: 99%

Gardnerella vaginalis: characteristics, clinical considerations, and controversies

1992

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The clinical significance, Gram stain reaction, and genus affiliation of Gardnerella vaginalis have been controversial since Gardner and Dukes described the organism as the cause of "nonspecific vaginitis," a common disease of women which is now called bacterial vaginosis. The organism was named G. vaginalis when taxonomic studies showed that it was unrelated to bacteria in various genera including Haemophilus and Corynebacterium. Electron microscopy and chemical analyses have elucidated the organism's gram-variable reaction. Controversy over the etiology of bacterial vaginosis was largely resolved by (i) studies using improved media and methods for the isolation and identification of bacteria in vaginal fluids and (ii) standardization of criteria for clinical and laboratory diagnosis. Besides G. vaginalis, Mobiluncus spp., Mycoplasma hominis, and certain obligate anaerobes are now acknowledged as participants in bacterial vaginosis. The finding that G. vaginalis, Mobiluncus spp., and M. hominis inhabit the rectum indicates a potential source of autoinfection in addition to sexual transmission. Extravaginal infections with G. vaginalis are increasingly recognized, especially when the toxic anticoagulant polyanetholesulfonate is omitted from blood cultures and when urine cultures are incubated anaerobically for 48 h. The finding that mares harbor G. vaginalis suggests that an equine model can be developed for studies of Gardnerella pathogenesis.

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Growth requirements ofHaemophilus vaginalis

Cited by 36 publications

References 14 publications

The growth of Gardnerella vaginalis and Lactobacillus acidophilus in Sorbarod biofilms

The growth of Gardnerella vaginalis and Lactobacillus acidophilus in Sorbarod biofilms

Characterisation and selection of a Lactobacillus species to re-colonise the vagina of women with recurrent bacterial vaginosis

Gardnerella vaginalis: characteristics, clinical considerations, and controversies

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