Growth, secondary metabolite production and antioxidant enzyme response of Morinda citrifolia adventitious root as affected by auxin and cytokinin

Baque, Md. Abdullahil; Hahn, Eun-Joo; Paek, Kee-Yoeup

doi:10.1007/s11816-009-0121-8

Cited by 74 publications

(28 citation statements)

References 30 publications

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“…In C. lanceolata, IBA positively affected the growth of roots and lancemaside A accumulation in the transgenic hairy roots. These results support the idea that IBA, a phytohormone, could affect secondary metabolism, particularly of triterpenoid compounds produced from various medicinal plants (Kim et al 2003;Baque et al 2010). …”

Section: Induction Of Hairy Rootssupporting

confidence: 84%

Triterpenoid production and phenotypic changes in hairy roots of Codonopsis lanceolata and the plants regenerated from them

Kim

Choi

2011

Plant Biotechnol Rep

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Codonopsis lanceolata (Campanulaceae) has been used in traditional medicines, as its roots contain several kinds of triterpenoid saponin with high medicinal values. In this work, we induced transgenic hairy roots of C. lanceolata and analyzed triterpenoid saponins from the hairy roots and hairy root-derived transgenic plants. Hairy roots were obtained from leaf explants by the transformation of Agrobacterium rhizogenes R1000. Transgenic hairy root lines were confirmed by the transcriptional activities of rolA, B, C, and D genes by RT-PCR. Transgenic root lines actively proliferated on hormone-free medium but not in nontransformed roots. Hairy roots contained richer triterpenoids (lancemaside A, foetidissimoside A, and aster saponin Hb) than nontransformed roots. Transgenic plants were regenerated from the hairy roots via somatic embryogenesis. They showed phenotypic alterations such as shortened shoots and an increased number of axillary buds and adventitious roots. The transgenic plants also contained higher triterpenoid levels than wild-type plants. These results suggest that hairy roots and transgenic plants of C. lanceolata could be used as medicinal materials for the production of triterpene saponins.

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Section: Induction Of Hairy Rootssupporting

confidence: 84%

Triterpenoid production and phenotypic changes in hairy roots of Codonopsis lanceolata and the plants regenerated from them

Kim

Choi

2011

Plant Biotechnol Rep

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“…Similarly, several workers have also used the liquid medium to establish the hairy root growth (He-Ping et al 2011;Pavlov et al 2005;Romero et al 2009;Sivanesan and Ryong Jeong 2009). Few previous studies support this idea that IBA could positively affect growth and secondary metabolism of various medicinal plants (Kim et al 2003;Baque et al 2010).…”

Section: Discussionmentioning

confidence: 96%

The influence of Agrobacterium rhizogenes on induction of hairy roots and ß-carboline alkaloids production in Tribulus terrestris L.

Sharifi

Sattari

Zebarjadi

et al. 2013

Physiol Mol Biol Plants

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We have developed an efficient transformation system for Tribulus terrestris L., an important medicinal plant, using Agrobacterium rhizogenes strains AR15834 and GMI9534 to generate hairy roots. Hairy roots were formed directly from the cut edges of leaf explants 10-14 days after inoculation with the Agrobacterium with highest frequency transformation being 49 %, which was achieved using Agrobacterium rhizogenes AR15834 on hormone-free MS medium after 28 days inoculation. PCR analysis showed that rolB genes of Ri plasmid of A. rhizogenes were integrated and expressed into the genome of transformed hairy roots. Isolated transgenic hairy roots grew rapidly on MS medium supplemented with indole-3-butyric acid. They showed characteristics of transformed roots such as fast growth and high lateral branching in comparison with untransformed roots. Isolated control and transgenic hairy roots grown in liquid medium containing IBA were analyzed to detect ß-carboline alkaloids by High Performance Thin Layer Chromatograghy (HPTLC).Harmine content was estimated to be 1.7 μg g −1 of the dried weight of transgenic hairy root cultures at the end of 50 days of culturing. The transformed roots induced by AR15834 strain, spontaneously, dedifferentiated as callus on MS medium without hormone. Optimum callus induction and shoot regeneration of transformed roots in vitro was achieved on MS medium containing 0.4 mg L −1 naphthaleneacetic acid and 2 mg L −1 6-benzylaminopurine (BAP) after 50 days. The main objective of this investigation was to establish hairy roots in this plant by using A. rhizogenes to synthesize secondary products at levels comparable to the wild-type roots.

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“…There are several lines of evidence suggesting that exogenous auxin increase enzyme activity during rooting or in tissue culture. It has also been demonstrated that exogenous auxin added to MS medium increased the antioxidant enzyme such as peroxidase and catalase (Tyburski et al 2009;Baque et al 2010). Auxin synthesis in the transformed hairy roots and also increasing its content in MS medium might be a signal for increased activity of antioxidant enzymes in the transformed roots.…”

Section: Discussionmentioning

confidence: 97%

Study of antioxidant enzymes activity and isozymes pattern in hairy roots and regenerated plants in Nicotiana tabacum

et al. 2011

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Hairy root disease is caused by the infection of wounded higher plants with Agrobacterium rhizogenes. Transformation of tissues or plants with A. rhizogenes, and with rol genes, as well as hairy roots may produce alterations in the plant secondary metabolism. H 2 O 2 and other ROS are involved as a signal in secondary metabolite production pathway and play a key role in plant defensive reactions. In this work, the effect of A. rhizogenes T-DNA on nicotine content, antioxidant enzymes activity, H 2 O 2 production, pattern of peroxidase (POX) and superoxide dismutase (SOD) isozymes in hairy roots and regenerated plants were studied. Rise in SOD and POX activities in the transformed lines of TRa and TRb and in the resultant regenerated plants, also the decreased level of H 2 O 2 in them, compared with the untransformed lines indicates that, the T-DNA genes expression of A. rhizogenes probably decreases H 2 O 2 level by increasing the production of antioxidant enzymes. Decrees the level of H 2 O 2 content in TRc line in spite of the similarity of antioxidant enzyme activity in comparison with normal root, indicate that A. rhizogenes activate other mechanisms except SOD and POX enzyme for reducing H 2 O 2 level.

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Growth, secondary metabolite production and antioxidant enzyme response of Morinda citrifolia adventitious root as affected by auxin and cytokinin

Cited by 74 publications

References 30 publications

Triterpenoid production and phenotypic changes in hairy roots of Codonopsis lanceolata and the plants regenerated from them

Triterpenoid production and phenotypic changes in hairy roots of Codonopsis lanceolata and the plants regenerated from them

The influence of Agrobacterium rhizogenes on induction of hairy roots and ß-carboline alkaloids production in Tribulus terrestris L.

Study of antioxidant enzymes activity and isozymes pattern in hairy roots and regenerated plants in Nicotiana tabacum

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