Growth stage and nitrate limiting response of NRT2 and NAR2 gene families of bread wheat, and complementation and retrieval of nitrate uptake of atnrt2.1 mutant by a wheat NRT2 gene

The Functional Diversity of the High-Affinity Nitrate Transporter Gene Family in Hexaploid Wheat: Insights from Distinct Expression Profiles

“…The diversity of NRT2 genes highlights the complexity of nitrate transport in plants. Recent publications focusing on bread wheat confirmed the presence of multiple genes in the wheat genome [ 26 , 27 , 28 ]. However, these studies have reported different numbers while proposing distinct nomenclatures, thus leading to inconsistencies and potential confusion in the wheat research community.…”

“…Taken together, it may be hypothesized that TaNRT2.1-2.6 genes play a central role in the NRT2 family in terms of being involved in the direct nitrate acquisition from soil, contributing to HATS. Kumar et al (2023) demonstrated that the expression of TaNRT2.1-6B (reported as TaNRT2.1-B6 ) restored the impaired N influx of Atnrt2.1 [ 26 ]. In addition, Wang et al (2019) found that Brachypodium nrt2.1 mutants significantly impair HATS, further supporting this hypothesis [ 9 ].…”

“…This table provides details on the identified wheat NRT2 and NRT3 genes, including their accession numbers, chromosomal locations, and length of encoded proteins. The corresponding gene names from previous studies [26][27][28] were aligned with the suggested nomenclature. A total of 11 NRT3-encoding genes were identified in the hexaploid wheat genome, forming four homoeologous subgroups (Table 1).…”

“…Furthermore, the homoeologous relationships between the genes present in the A, B, and D wheat sub-genomes have been described. A new nomenclature is proposed that accounts for these homoeologous subgroups (Table 1), and a conversion table is presented, thereby aligning the present nomenclature with that of recent publications [26][27][28]. This resource aims to facilitate future research and avoid inconsistencies in gene names.…”

“…Hence, the aim of this study, is to identify and characterize the phylogenetic relations of the hexaploid wheat NRT2 and NRT3 genes compared with their orthologs from Arabidopsis, Brachypodium, rice, and barley ( Hordeum vulgare ). Three recent studies have also focused on the NRT2s in wheat [ 26 , 27 , 28 ]; however, each study suggested different nomenclature for the identified NRT2s . The presence of different names for the same gene accession can cause confusion and is a major problem in the wheat research community.…”