Growth Substrate and Prophage Induction Collectively Influence Metabolite and Lipid Profiles in a Marine Bacterium

Basso, Jonelle T. R.; Jones, Katarina A.; Jacobs, Kaylee R.; Christopher, Courtney J.; Fielland, Haley B.; Campagna, Shawn R.; Buchan, Alison

doi:10.1128/msystems.00585-22

Cited by 3 publications

(3 citation statements)

References 63 publications

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“…Furthermore, bacterial culture conditions strongly influence the production of secondary metabolites. For example, Basso et al ( 38 ) demonstrated that metabolites and lipids produced by two Sulfitobacter strains were strongly determined by growth medium composition, with only 15% of the 175 measured metabolites overlapping across different culture media. In our assay, we observed that bacteria such as Micromonospora sp.…”

Section: Discussionmentioning

confidence: 99%

Uncovering Genomic Features and Biosynthetic Gene Clusters in Endophytic Bacteria from Roots of the Medicinal Plant Alkanna tinctoria Tausch as a Strategy To Identify Novel Biocontrol Bacteria

et al. 2023

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Section: Discussionmentioning

confidence: 99%

Uncovering Genomic Features and Biosynthetic Gene Clusters in Endophytic Bacteria from Roots of the Medicinal Plant Alkanna tinctoria Tausch as a Strategy To Identify Novel Biocontrol Bacteria

et al. 2023

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“…Intriguingly, the presence of SW1 also decreased the lateral flagellar synthesis and swarming motility of S. piezotolerans WP3 at low temperature ( 30 ). Recently, marine prophages have been shown to influence the host bacterium’s lipid and metabolite profiles ( 31 ).…”

Section: Introductionmentioning

confidence: 99%

Prophage enhances the ability of deep-sea bacterium Shewanella psychrophila WP2 to utilize D-amino acid

Tan,

Zhang,

Liu

et al. 2024

Microbiol Spectr

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Prophages are prevalent in the marine bacterial genomes and reshape the physiology and metabolism of their hosts. However, whether and how prophages influence the microbial degradation of D-amino acids (D-AAs), which is one of the widely distributed recalcitrant dissolved organic matters (RDOMs) in the ocean, remain to be explored. In this study, we addressed this issue in a representative marine bacterium, Shewanella psychrophila WP2 (WP2), and its integrated prophage SP1. Notably, compared to the WP2 wild-type strain, the SP1 deletion mutant of WP2 (WP2ΔSP1) exhibited a significantly lower D-glutamate (D-Glu) consumption rate and longer lag phase when D-Glu was used as the sole nitrogen source. The subsequent transcriptome analysis identified 1,523 differentially expressed genes involved in diverse cellular processes, especially that multiple genes related to inorganic nitrogen metabolism were highly upregulated. In addition, the dynamic profiles of ammonium, nitrate, and nitrite were distinct between the culture media of WP2 and WP2ΔSP1. Finally, we provide evidence that SP1 conferred a competitive advantage to WP2 when D-Glu was used as the sole nitrogen source and SP1-like phages may be widely distributed in the global ocean. Taken together, these findings offer novel insight into the influences of prophages on host metabolism and RDOM cycling in marine environments. IMPORTANCE This work represents the first exploration of the impact of prophages on the D-amino acid (D-AA) metabolism of deep-sea bacteria. By using S. psychrophila WP2 and its integrated prophage SP1 as a representative system, we found that SP1 can significantly increase the catabolism rate of WP2 to D-glutamate and produce higher concentrations of ammonium, resulting in faster growth and competitive advantages. Our findings not only deepen our understanding of the interaction between deep-sea prophages and hosts but also provide new insights into the ecological role of prophages in refractory dissolved organic matter and the nitrogen cycle in deep oceans.

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“…Conversely, the lysis and release of sticky transparent exopolymeric particles (TEPs) can lead to enhanced sinking of organic material from upper to lower oceanic layers, known as the “viral shuttle” ( 10 , 11 ). Also, lysogenic infections by temperate phages can occur, which likely lead to long-term associations with the respective host and modulation of host physiology (e.g., morphology, gene expression, and metabolism) ( 12 , 13 ). Although the presence and influence of lysogenic infections are significant in marine systems ( 14 – 16 ), lysogeny itself is very complex and little is known about the induction of the lytic form.…”

Section: Introductionmentioning

confidence: 99%

Grazing on Marine Viruses and Its Biogeochemical Implications

Mayers

Kuhlisch

Basso

et al. 2023

mBio

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Growth Substrate and Prophage Induction Collectively Influence Metabolite and Lipid Profiles in a Marine Bacterium

Abstract: Community-level metabolomics approaches are increasingly used to characterize natural microbial populations. These approaches typically depend upon temporal snapshots from which the status and function of communities are often inferred.

Cited by 3 publications

References 63 publications

Uncovering Genomic Features and Biosynthetic Gene Clusters in Endophytic Bacteria from Roots of the Medicinal Plant Alkanna tinctoria Tausch as a Strategy To Identify Novel Biocontrol Bacteria

Uncovering Genomic Features and Biosynthetic Gene Clusters in Endophytic Bacteria from Roots of the Medicinal Plant Alkanna tinctoria Tausch as a Strategy To Identify Novel Biocontrol Bacteria

Prophage enhances the ability of deep-sea bacterium Shewanella psychrophila WP2 to utilize D-amino acid

Grazing on Marine Viruses and Its Biogeochemical Implications

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