Growth temperature is involved in the regulation of extracellular lipase at two different levels in Pseudomonas fluorescens strain MF0

Guillou, Catherine; Merieau, Annabelle; Trebert, B.; Guespin, Michel J. F.

doi:10.1007/bf00130793

Cited by 10 publications

(6 citation statements)

References 12 publications

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“…Previous studies demonstrated that 17ЊC was the optimal temperature for the production of several exported enzymes and the expression of at least two of the corresponding genes (5,7,8,12,20). This temperature has now been shown to have a broader significance, since it was identified as the limit between a cold domain (0 to 17ЊC) and a suboptimal domain (17 to 30ЊC).…”

Section: Discussionmentioning

confidence: 98%

“…Cells were collected in mid-exponential phase and early stationary phase and assayed for determination of cellular proteins and RNA, and cell size was measured. Continuous cultures were carried out in MMC under carbon limitation as previously described (8). Four residence times were allowed after each modification of growth temperature or dilution rate, so that steady-state conditions were achieved.…”

Section: Methodsmentioning

confidence: 99%

“…Although coregulated by the growth temperature, production of these enzymes is variously dependent on other growth conditions such as the medium composition and growth phase (5,12). Furthermore, the kinetics of production of lipase and protease as a function of growth rate are dependent on the temperature (8). The permeability of the outer membrane to the ␤-lactamine mezlocillin is also modified around 17ЊC (22).…”

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confidence: 99%

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Evidence for two domains of growth temperature for the psychrotrophic bacterium Pseudomonas fluorescens MF0

Guillou¹,

Guespin‐Michel²

1996

Appl Environ Microbiol

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The variations in the maximal specific growth rate of the psychrotrophic bacterium Pseudomonas fluorescens MF0 with respect to temperature were studied between 0 and 30؇C (optimal for growth). The Arrhenius plot showed a drastic change in slope at the intermediate temperature of 17؇C. Over the cold domain from 0 to 17؇C, the temperature characteristic was twofold higher than over the suboptimal domain from 17 to 30؇C. The macromolecular composition of exponentially growing cells was invariant over the entire range from 0 to 30؇C. Variations of temperature and growth rate were independently investigated through chemostat experiments in order to characterize their respective effects on cell macromolecular composition and size. The effect of growth rate in this psychrotrophic strain is identical to that of all other bacteria assayed so far. In contrast, an original biphasic variation of total protein concentration was demonstrated in strain MF0 with respect to temperature, with a maximum at 17 to 20؇C. Indeed, increasing the temperature in the chemostat resulted in a biphasic decrease in the net protein production rate: a very slight decrease below 17؇C and a much larger decrease from 17 to 28؇C. These results could signify an increase in the cellular protein degradation rate with increasing temperature, especially above 17؇C.

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Section: Discussionmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Evidence for two domains of growth temperature for the psychrotrophic bacterium Pseudomonas fluorescens MF0

Guillou¹,

Guespin‐Michel²

1996

Appl Environ Microbiol

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“…It was suggested by Hadas et al [6] that cell size rather than metabolic rate is one of the factors that affect T4 activity against E. coli. In the case of P. fluorescens the growth temperature influences cell size and shape [23]. Furthermore, at the optimal growth temperature the cells exhibit a greater concentration of proteins and RNA [24].…”

Section: Discussionmentioning

confidence: 99%

Pseudomonas fluorescensinfection by bacteriophage Î¦S1: the influence of temperature, host growth phase and media

Sillankorva

Oliveira

Vieira

et al. 2004

FEMS Microbiology Letters

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The influence of host growth temperature, phase and media, together with the effect of infection temperature on bacteriophage PhiS1 infection of Pseudomonas fluorescens were examined. The rates of cell lysis and phage release were determined and showed that the efficacy of phage infection was optimal with host cells grown and infected at 26 degrees C. The host physiological state also affected these rates. Infection was dependent on the presence of cell wall proteins with molecular weights of 17.5+/-1 and 99+/-5 kDa.

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“…Keeping these restrictions in mind, Morita's definitions remain perfectly valid providing an optimal physiological temperature is defined. This concept has been successfully explored during the study of psychrotrophic Pseudomonas [29][30][31][32]. For these species a critical temperature, close to the break point of an Arrhenius plot of growth rates, separates two domains of temperature characterized by the rates of exoenzymes production, the rates of protein accumulation and the permeability of the outer membrane.…”

Section: Psychrophily: An Evolving Conceptmentioning

confidence: 99%

Psychrophilic enzymes: molecular basis of cold adaptation

Feller

Gerday

1997

CMLS, Cell. mol. life sci.

371

233

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Abstract. Psychrophilic organisms have successfully provides enhanced abilities to undergo conformational colonized polar and alpine regions and are able to grow changes during catalysis. Thermal instability of coldefficiently at sub-zero temperatures. At the enzymatic adapted enzymes is therefore regarded as a consequence level, such organisms have to cope with the reduction of of their conformational flexibility. A survey of the psychrophilic enzymes studied so far reveals only minor chemical reaction rates induced by low temperatures in alterations of the primary structure when compared to order to maintain adequate metabolic fluxes. Thermal compensation in cold-adapted enzymes is reached mesophilic or thermophilic homologues. However, all known structural factors and weak interactions inthrough improved turnover number and catalytic effivolved in protein stability are either reduced in number ciency. This optimization of the catalytic parameters can originate from a highly flexible structure which or modified in order to increase their flexibility.Key words. Psychrophiles; extremophiles; cold adaptation; microbial proteins; protein stability; weak interactions; Antarctic.Psychrophiles live at the lowest temperatures which allow the development of living organisms. These extremophilic organisms, either prokaryotic or eukaryotic, represent a major class of the living world if we consider the vast extent of permanently cold environments on Earth, such as polar and alpine regions or deep-sea waters. The successful colonization of such severe ecological niches by psychrophiles, their diversity and abundance are now well recognized. The lower temperature limit of life is commonly defined as the freezing point of cellular water. Although apparently simple by definition, this limit can drop significantly below 0°C, the freezing point of pure water. For instance, the freezing point of a typical marine teleost ranges between −0.5 and −0.9°C. Sodium chloride is responsible for about 85% of the freezing point depression, and the remaining 15% is due to other small solutes. Synthesis of antifreeze glycoproteins and peptides can further depress the freezing point of body fluids or cellular water. These proteins lower the freezing point by a noncolligative mechanism without altering the melting point significantly, and are therefore also referred to as thermal hysteresis proteins [1,2]. They were first discovered in the blood sera of Antarctic fish living perennially at about −1.9°C, but have been now reported in many organisms including plants, insects, fungi and bacteria [3][4][5]. Levels of thermal hysteresis activity generally range from 0.1 to 0.3°C in bacteria, from 0.2 to 0.5°C in plants, from 0.7 to 1.5°C in fish and from 3 to 6°C in insects. Besides the synthesis of cryoprotectants which lead to freeze avoidance, several organisms have developed mechanisms of freeze tolerance, involving drastic metabolic modifica-* Corresponding author.

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Growth temperature is involved in the regulation of extracellular lipase at two different levels in Pseudomonas fluorescens strain MF0

Cited by 10 publications

References 12 publications

Evidence for two domains of growth temperature for the psychrotrophic bacterium Pseudomonas fluorescens MF0

Evidence for two domains of growth temperature for the psychrotrophic bacterium Pseudomonas fluorescens MF0

Pseudomonas fluorescensinfection by bacteriophage Î¦S1: the influence of temperature, host growth phase and media

Psychrophilic enzymes: molecular basis of cold adaptation

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