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Grafting is an ancient technique especially used in horticulture and in orchards as a tool for plant propagation and improvement. Although grafting has brought incredible benefits to agriculture and is being used worldwide in the cultivation of almost every fruit tree, the mechanisms, and pathways driving the formation or the incompatibilities of graft unions are still largely unknown. Recently, the potential of grafting has resurfaced in the literature and molecular approaches are currently contributing to the understanding of graft union formation and the phenomenon of scion–rootstock graft incompatibility. This work focuses on the cellular and molecular mechanisms of graft formation in woody plants, highlighting the identified regulators of this process in molecular terms. We review how the healing of the union proceeds in incompatible grafts, the challenge of phenotyping graft incompatibility, and the detection methods developed for its prediction. In the pursuit of finding sustainable solutions for agriculture, while faced with the alarming predictions of climate changes, grafting is being revaluated as a new biotechnological tool. Therefore, some examples of how we can broaden the scope of useful grafting applications, exploit biodiversity in a unique chimeric plant, and manipulate phenotypic traits by delivering proteins and RNAs across graft unions, are also discussed. Research in this field still has a long way to go before the exciting prospect of deliberately combining multiple plant traits becomes reality.
Grafting is an ancient technique especially used in horticulture and in orchards as a tool for plant propagation and improvement. Although grafting has brought incredible benefits to agriculture and is being used worldwide in the cultivation of almost every fruit tree, the mechanisms, and pathways driving the formation or the incompatibilities of graft unions are still largely unknown. Recently, the potential of grafting has resurfaced in the literature and molecular approaches are currently contributing to the understanding of graft union formation and the phenomenon of scion–rootstock graft incompatibility. This work focuses on the cellular and molecular mechanisms of graft formation in woody plants, highlighting the identified regulators of this process in molecular terms. We review how the healing of the union proceeds in incompatible grafts, the challenge of phenotyping graft incompatibility, and the detection methods developed for its prediction. In the pursuit of finding sustainable solutions for agriculture, while faced with the alarming predictions of climate changes, grafting is being revaluated as a new biotechnological tool. Therefore, some examples of how we can broaden the scope of useful grafting applications, exploit biodiversity in a unique chimeric plant, and manipulate phenotypic traits by delivering proteins and RNAs across graft unions, are also discussed. Research in this field still has a long way to go before the exciting prospect of deliberately combining multiple plant traits becomes reality.
Abbreviations: ABS/RC -energy absorption flux; DI0/RC -dissipated energy flux per reaction center; ET0/RC -photosynthetic electron transport flux per reaction center; F0 -minimum fluorescence yield of the dark-adapted state; Fm -maximum fluorescence; PI(ABS)photosynthetic efficiency performance index; RC/CSm -density of reaction center per maximum cross-section; TR0/RC -trapped energy flux; φD0 -quantum yield of energy dissipation; φP0 -maximum quantum yield of primary photochemistry.
Rootstocks are fundamental for peach production, and their architectural root traits determine their performance. Root-system architecture (RSA) analysis is one of the key factors involved in rootstock selection. However, there are few RSA studies on Prunus spp., mostly due to the tedious and time-consuming labor of measuring below-ground roots. A root-phenotyping experiment was developed to analyze the RSA of seedlings from ‘Okinawa’ and ‘Guardian’TM peach rootstocks. The seedlings were established in rhizoboxes and their root systems scanned and architecturally analyzed. The root-system depth:width ratio (D:W) throughout the experiment, as well as the root morphological parameters, the depth rooting parameters, and the root angular spread were estimated. The ‘Okinawa’ exhibited greater root morphological traits, as well as the other parameters, confirming the relevance of the spatial disposition and growth pattern of the root system.
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