The objective of the study was to study the influence of application of liquid jeevamrutha at different dosage and frequency on fruit quality and biochemical characteristics of guava cv. L-49. The experiment was designed in two factorial randomized block design with each factor having three levels. Three different levels of liquid jeevamrutha viz., 500 l/ha, 750 l/ha and 1000 l/ha at different frequencies like 15 days, 21 days and 30 days was applied to guava trees in the effective root zone area. The combination effect of dosage and frequency of application was compared with recommended package of practice (RPP). The results showed that, application of higher dosage of liquid jeevamrutha i.e., 1000 l/ha at an interval of 15 days recorded improved quality and biochemical attributes. Similarly in interactions also D3F1 (Application of liquid jeevamrutha @ 1000 l/ha at 15 days interval) recorded higher TSS (12.40 O Brix), TSS to acid ratio (31.44), ascorbic acid (156.25 mg/100 g), reducing sugars (4.68%), non-reducing sugars (2.69%), total sugars (7.37%), shelf life (7.67 days).When the interactions were compared with control treatment (RPP) significantly higher TSS, sugar content, ascorbic acid and shelf life was recorded in control treatment.