Balanced plant nutrition is essential to achieve high yields of canola (Brassica napus L.) and get the best economic return from applied fertilizers. A field study was conducted at nine site‐years across eastern Canada to investigate the effects of nitrogen (N), sulfur (S) and boron (B) fertilization on canola nutrient uptake, nutrient balance, and their relationship to canola yields. The factorial experiment consisted of four N rates of 0 (N0), 50 (N50), 100 (N100), and 150 (N150) kg ha−1, two S rates of 0 (S0) and 20 (S20) kg ha−1, and three B treatments of 0 (B0), 2 kg ha−1 at preplant (B2.0P), and 0.5 kg B ha−1 foliar‐applied at early flowering stage (B0.5F). Each site‐year used the same experimental design and assigned treatments in a randomized complete block design with four replications. Fertilizer S application greatly improved seed yields at six out of nine site‐years, and the highest N use efficiency was in the N150+S20 treatment. Sulfur application generally increased seed S concentration, seed S removal, and plant total S uptake, while B fertilization mainly elevated straw B concentration and content, with minimal effect on seed yields. At the early flowering stage, plant tissue S ranged from 2.2 to 6.6 mg S g−1, but the N : S ratio was over or close to the critical value of 12 in the N150+S0 combination at five site‐years. On average across nine site‐years, canola reached a plateau yield of 3580 kg ha−1 when plants contained 197 kg N ha−1, 33 kg S ha−1 and 200 g B ha−1, with a seed B content of 60 g B ha−1. The critical N, S, and B values identified in this work and their potential for a posteriori nutrient diagnosis of canola should be useful to validate fertilizer requirements for canola production in eastern Canada.