GSH-Triggered/Photothermal-Enhanced H2S Signaling Molecule Release for Gas Therapy

Abstract

Traditional treatment methods for tumors are inefficient and have severe side effects. At present, new therapeutic methods such as phototherapy, chemodynamic therapy, and gasodynamic therapy have been innovatively developed. High concentrations of hydrogen sulfide (H2S) gas exhibit cancer-suppressive effects. Herein, a Prussian blue-loaded tetra-sulfide modified dendritic mesoporous organosilica (PB@DMOS) was rationally constructed with glutathione (GSH)-triggered/photothermal-enhanced H2S signaling molecule release properties for gas therapy. The as-synthesized nanoplatform confined PB nanoparticles in the mesoporous structure of organosilica silica due to electrostatic adsorption. In the case of a GSH overexpressed tumor microenvironment, H2S gas was controllably released. And the temperature increases due to the photothermal effects of PB nanoparticles, further enhancing H2S release. At the same time, PB nanoparticles with excellent hydrogen peroxide catalytic performance also amplified the efficiency of tumor therapy. Thus, a collective nanoplatform with gas therapy/photothermal therapy/catalytic therapy functionalities shows potential promise in terms of efficient tumor therapy.

The Antioxidant Properties of Glucosinolates in Cardiac Cells Are Independent of H2S Signaling

“…Differently from our hypothesis, no H 2 S release was detected from GSLs under various conditions. It has been reported that sulfur-containing amino acids could react with sulfur-containing natural products to release H 2 S non-enzymatically [12,14]. However, our studies show that the co-incubation of three sulfur-containing amino acids (cysteine, NAC, and GSH) with GSLs did not induce H 2 S release.…”

“…Enzymatically, cystathionine beta-synthase, cystathionine gamma-lyase (CSE), and 3-mercaptopyruvate sulfurtransferase, along with cysteine aminotransferase, use the cofactor pyridoxal 5 ′ -phosphate (P5P) to catalyze cysteines and produce H 2 S [11]. Non-enzymatically, H 2 S can be released from sulfur-containing amino acids and natural sulfur products via chemical interaction with P5P, glutathione (GSH), and/or iron [12,13]. Other sulfur-containing molecules, i.e., diallyldisulfide from garlic, can release H 2 S in the bloodstream through interaction with GSH [12,14].…”

“…Non-enzymatically, H 2 S can be released from sulfur-containing amino acids and natural sulfur products via chemical interaction with P5P, glutathione (GSH), and/or iron [12,13]. Other sulfur-containing molecules, i.e., diallyldisulfide from garlic, can release H 2 S in the bloodstream through interaction with GSH [12,14]. ITCs (the byproducts of GSLs) and glucoraphanin (a GSL) have been shown to release H 2 S in mammalian cells in a cysteine-dependent non-enzymatic reaction [15,16].…”