GSTM1 and GSTT1 polymorphisms in endometriosis in women from Goiás, Brazil

Frare, Ariane Bocaletto; Barbosa, A.M.; Costa, Iasmim Ribeiro da; Souza, Sandro Rodrigo Vale de; Silva, R.C.P.C.; Bordin, B.M.; Júnior, C.L. Ribeiro; Moura, K.K.V.O.

doi:10.4238/2013.august.2.1

Cited by 16 publications

(23 citation statements)

References 15 publications

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“…A lower prevalence (27.6%) was reported by Chen et al (1996) in the US. Other researchers detected a GSTM1-null polymorphism prevalence of 74.0% in women (Frare et al, 2013). Similar results showed that the GSTM1-null polymorphism was 73.8% in African-Americans (Weiserbs et al, 2003).…”

Section: Discussionsupporting

confidence: 62%

Polymorphisms of GSTM1, GSTT1, and p53 in Goiânia, Goiás

Rodrigues¹,

Costa²,

Martins³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Genetic polymorphisms are defined as changes within the DNA sequences of genes that have frequencies in the population higher than 1%. The glutathione S-transferases play an important role in the cellular detoxification systems involved in oxidative stress that can lead to accumulation of reactive oxygen species. Epidemiological studies have suggested that individuals with homozygous deletion of glutathione S-transferase mu 1 (GSTM1) and glutathione S-transferase theta 1 (GSTT1) are at higher risk of developing several types of neoplasias. The p53 protein is highly expressed in tumors and transformed cells, and the p53 is a classical tumor suppressor gene involved in regulating cell growth and development. In this study, we investigated the prevalence of polymorphisms in the p53, GSTM1, and GSTT1 genes in a population from Goiânia. We evaluated the polymorphisms of these genes in peripheral blood samples. The null or present polymorphism of GSTM1 and GSTT1 17359Polymorphisms of GSTM1, GSTT1, and p53 in Goiânia, Goiás ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 17358-17365 (2015) genes and Arg/Pro of the p53 gene were analyzed. Our results revealed a higher frequency of the GSTM1-null polymorphism (72.4%) than the GSTM1-present genotype (27.6%). For GSTT1, we observed higher frequency for the null genotype (65.5%) compared to the present genotype (34.5%). Analysis of p53 gene polymorphisms showed a higher frequency for the genotype Arg/Pro (66%) and a lower frequency for the Arg/Arg (23%) and Pro/Pro (11%) genotypes. It is essential to understand polymorphism frequencies in different populations and to evaluate the role of genetic polymorphisms and their effects on health.

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Section: Discussionsupporting

confidence: 62%

Polymorphisms of GSTM1, GSTT1, and p53 in Goiânia, Goiás

Rodrigues¹,

Costa²,

Martins³

et al. 2015

Genet. Mol. Res.

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“…The DNA was stored at -20°C until amplified by the polymerase chain reaction (PCR) method in order to verify the eNOS polymorphism. To avoid contamination of the samples, the analysis was performed in an appropriate laminar flow according to the protocol proposed by Frare et al (2013).…”

Section: Methodsmentioning

confidence: 99%

Polymorphism of the gene eNOS G894T (Glu298Asp) in symptomatic patients with aterosclerosis

Campedelli¹,

Silva²,

Rodrigues³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Atherosclerotic and its cardiovascular complications are responsible for 17.5 million deaths a year, according to the World Health Organization. There is consensus that atherosclerosis involves multiple pathogenic processes initiated by endothelial dysfunction, with inflammation and vascular proliferation determining alterations in the matrix, with consequent formation of the atheromatous plaque and its clinical implications. Risk factors such as hypertension, diabetes mellitus, dyslipidemia, and smoking are widely known. Currently, genotyping, which is not directly related to these factors, is not accepted to estimate the risk of cardiovascular diseases, but strong evidence indicates several polymorphic genes as factors of risk and progression leading to complications of the disease. Among the genes involved, eNOS (endothelial nitric oxide synthase gene), which is responsible for the production of endothelial nitric oxide (an important arterial vasodilator), when presented in polymorphic variation can determine production, malfunction, and predisposition to atherosclerosis. In the present study, we analyzed the G894T polymorphism of the eNOS gene in groups of individuals diagnosed with atherosclerosis and in a control group. We collected 200 blood samples from patients previously diagnosed with atherosclerosis and 100 samples formed the control group. The genotyping analysis for polymorphism of the eNOS gene was determined by PCR. We considered variables such as gender, smoking, smoking history, and alcohol consumption; statistical differences were found in the distribution of case and control groups (P = 0.0378) and in non-smoking patients (P = 0.0263). In the other associations, no statistically significant difference was found. In the population studied, the frequency of the heterozygous genotype (GT) was much higher than in the other populations (GG and TT) in both groups (case and control). The GG genotype showed greater susceptibility to atherosclerosis. Association of the GG genotype in non-smokers also showed greater susceptibility. Gender, alcohol consumption, smoking, and smoking history did not influence atherosclerosis.

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“…The whole procedure was conducted in a laminar flow hood to minimize contamination. The final volume of the PCR was 25 µL according to the protocol proposed by Frare et al (2013).…”

Section: Methodsmentioning

confidence: 99%

GSTM1 polymorphism in patients with clinical manifestations of atherosclerosis

et al. 2017

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ABSTRACT. Atherosclerosis is characterized by lesions, called atheroma or atheromatous plaques, in the inner layer of blood vessels, which block the vascular lumen and weaken the underlying tunica media. Several modifiable and non-modifiable risk factors for the development of atherosclerosis exist. The modifiable risk factors include hypertension, smoking, obesity, high LDL and low HDL cholesterol levels, sedentary lifestyle, and stress; the non-modifiable factors include diabetes mellitus, family history of hypertension and heart disease, thrombophilia, sex, age, and genetic factors. The association of polymorphisms in GST with coronary artery disease has been studied since the polymorphisms can affect enzyme activity and contribute to the onset of atherosclerosis. We analyzed polymorphisms in GSTM1 in individuals diagnosed with atherosclerosis as well as in healthy individuals (control group). The frequency of the GSTM1 present genotype in the atherosclerosis group was 1.2 times higher than that observed in the control group. We found no sex-or alcoholconsumption-dependent differences between the occurrences of the present and null genotypes. However, the GSTM1 present genotype occurred in 52

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GSTM1 and GSTT1 polymorphisms in endometriosis in women from Goiás, Brazil

Cited by 16 publications

References 15 publications

Polymorphisms of GSTM1, GSTT1, and p53 in Goiânia, Goiás

Polymorphisms of GSTM1, GSTT1, and p53 in Goiânia, Goiás

Polymorphism of the gene eNOS G894T (Glu298Asp) in symptomatic patients with aterosclerosis

GSTM1 polymorphism in patients with clinical manifestations of atherosclerosis

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