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Abstract

Comparação in vitro entre técnicas de enteroanastomose em jejuno de equinos hígidos Abstract: Several techniques of small intestine anastomoses have been proposed and, until these days the most accepted, technique between horses surgeons is the one with two-layer pattern, with an inversion suture pattern as the last layer. This study aim to evaluate, in vitro, three anastomoses techniques, chosen from a questionnaire previously sent to the main centers of reference in equine surgery in Brazil. For this, this experiment consisted of two phases. On the first phase, a questionnaire was sent to several Brazilian equine surgeons, in order to decide which techniques and suture methods are most performed for jejunojejunostomy. The most frequently cited suture patterns were simples continuous overlapped with cushing, one layer simples continuous and one layer interrupted lembert. Phase 2 consisted on evaluating the biomechanical resistance, construction time and change in luminal circumference of the three most cited suture patterns in the questionnaire. For this, three groups were assembled: G1 (simples continuous overlap by cushing), G2 (one layer simples continuous) and G3 (one layer interrupted lembert). A control group (GC) was tested for each sample evaluated. 32 segments of 16 healthy horses were collected after slaughter. The specimens were rinsed and frozen separately in lactated ringer solution. To perform the experiment, each segment was thawed immersed in lactated ringer solution at room temperature and subjected to testing within 2 hours after thawing.. Prior to suturing, the mean of the external hemicircumference of each segment was obtained. After performing anastomoses, the external hemicircumference of the anastomoses was mesured and compared to the mean previously obtained. Time of construction was recorded for each group tested. The biomechamical resistence test was performed, and subsequently, the mean of luminal hemicircumference of the anastomosed segments were obtained and compared to the luminal hemicircumference of the anastomoses site. The failure location was recorded for evaluation. The variables were analyzed using repeated measures ANOVA with subsequent application of TUKEY test. Statistical significance was set at p <0,05. All groups construction times showed statistical significance. G1 showed mean of 24.74 (± 4.18) minutes, G2 14.13 (± 2.97) minutes and G3 23.83 (± 6.23) minutes. G1 and G2 groups showed no statistical difference in the maximum pressure reached, with 99.13 mmHg (± 42.82) and