1985
DOI: 10.1073/pnas.82.13.4316
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GTPase of bovine rod outer segments: the amino acid sequence of the alpha subunit as derived from the cDNA sequence.

Abstract: The sequence of the 350 amino acids in the a subunit of GTPase of bovine rod outer segments has been determined. Enriched GTPase mRNA was used to prepare a cDNA library in the expression vector Xgtll and several overlapping cDNA clones corresponding to the a subunit of the GTPase were identified. The cDNA sequence determined contains 93 nucleotides upstream of the 5' end of the coding region, 1050 nucleotides that specify the amino acid sequence, and 45 nucleotides downstream from the 3' end. The previously de… Show more

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Cited by 151 publications
(27 citation statements)
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“…Furthermore, a missense mutation in the yeast RAS2 gene analogous to the position-12 activating mutation in human p21 causes a marked stimulation of yeast adenylate cyclase and cyclic AMP levels, the effect of which is a reduction in cell viability after nutrient deprivation (36). These observations have fueled speculations that p21 may be functionally similar or equivalent to the yeast ras proteins and to the family of G proteins that transduce extracellular signals across vertebrate membranes, a notion further supported by homologies in amino acid sequence (19,25,34,39) and function (9,16,20,35) among human p2is, yeast RAS proteins, and the family of mammalian G proteins. Despite these indications, it must be stressed that there is yet no compelling evidence that human ras genes, as part of an extended family whose functions may have expanded and diverged considerably, encode proteins that directly participate in the control of mammalian adenylate cyclase.…”
mentioning
confidence: 89%
“…Furthermore, a missense mutation in the yeast RAS2 gene analogous to the position-12 activating mutation in human p21 causes a marked stimulation of yeast adenylate cyclase and cyclic AMP levels, the effect of which is a reduction in cell viability after nutrient deprivation (36). These observations have fueled speculations that p21 may be functionally similar or equivalent to the yeast ras proteins and to the family of G proteins that transduce extracellular signals across vertebrate membranes, a notion further supported by homologies in amino acid sequence (19,25,34,39) and function (9,16,20,35) among human p2is, yeast RAS proteins, and the family of mammalian G proteins. Despite these indications, it must be stressed that there is yet no compelling evidence that human ras genes, as part of an extended family whose functions may have expanded and diverged considerably, encode proteins that directly participate in the control of mammalian adenylate cyclase.…”
mentioning
confidence: 89%
“…Amino acid sequence analysis of one of the tryptic peptides derived from rat brain Gia revealed the sequence of 17 amino acids: Glu-Tyr-Gln-Leu-Asn-Asp-Ser-Ala-Ala-Tyr-Tyr-LeuAsn-Asp-Leu-Xaa-Arg. The 51-base oligonucleotide probe (5' GCGGTCCAGGTCGTTGAGATAGTAGGCAGCAGA-GTCGTTGAGCTGGTACTC 3') corresponding to this amino acid sequence was designed by assuming the third letter of codons on the basis of the codon usage of known rat brain proteins (28) and of bovine Ta (17)(18)(19)(20). We assumed the residue Xaa to be Asp, to be homologous with the amino acid Proc.…”
Section: Methodsmentioning
confidence: 99%
“…Thus, the nucleotide sequences of cDNA coding for Ta have been determined in several laboratories (17)(18)(19)(20), and those for y (21,22) and (3 (23) subunits have also been reported. Harris et al (24) have obtained a bovine brain cDNA clone encoding most of Ga. In this paper we report the cloning and sequence determination of cDNAs coding for a subunits of G,, Gi, and Go proteins from rat C6 glioma cells.…”
mentioning
confidence: 99%
“…The results presented here are consistent with these predictions and additionally suggest that Lys-117 is important for guanine nucleotide binding. In addition to the rasH amino acid sequence 110 through 120, sequence alignment comparisons identified three other principal regions of significant homology between ras and other GTP-binding proteins, including transducin and the bacterial elongation (EF-Tu and EF-G) and initiation (IF2) factors (15,23,36). These three regions of homology are represented by the cellular ras amino acid residues (i) 5 to 15, (ii) 55 to 65, and (iii) 72 to 85, and they appear in the same order in a-transducin and the bacterial elongation factors EF-Tu and EF-G.…”
mentioning
confidence: 99%