Guapiaçu virus, a new insect-specific flavivirus isolated from two species of Aedes mosquitoes from Brazil

Ribeiro, Geovani de Oliveira; Costa, Antônio Charlys da; Gill, Danielle Elise; Ribeiro, Edcelha Soares D’Athaide; Rêgo, Márlisson Octávio da Silva; Monteiro, Fred Júlio Costa; Villanova, Fabiola; Nogueira, Juliana Silva; Maeda, Adriana Yurika; Souza, Renato Pereira de; Tahmasebi, Roozbeh; Morais, Vanessa dos Santos; Pandey, Ramendra Pati; Raj, V. Samuel; Scandar, Sirle Abdo Salloum; Vasami, Fernanda Gisele da Silva; D'Agostino, Leandro Guaraglia; Maiorka, Paulo César; Deng, Xutao; Nogueira, Maurício Lacerda; Sabino, Éster Cerdeira; Delwart, Eric; Leal, Élcio; Cunha, Mariana Sequetin

doi:10.1038/s41598-021-83879-6

Cited by 14 publications

(14 citation statements)

References 64 publications

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“…Some of the most important vector-borne viruses that infect humans and animals are members of the family Flaviviridae . The family is divided into distinct clusters that contain either vertebrate or invertebrate-specific viruses ( de Oliveira Ribeiro et al, 2021 ). A flavivirus that clustered together with invertebrate-specific viruses was identified in C. circumscriptus .…”

Section: Discussionmentioning

confidence: 99%

Dissecting the Species-Specific Virome in Culicoides of Thrace

et al. 2022

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Biting midges (Culicoides) are vectors of arboviruses of both veterinary and medical importance. The surge of emerging and reemerging vector-borne diseases and their expansion in geographical areas affected by climate change has increased the importance of understanding their capacity to contribute to novel and emerging infectious diseases. The study of Culicoides virome is the first step in the assessment of this potential. In this study, we analyzed the RNA virome of 10 Culicoides species within the geographical area of Thrace in the southeastern part of Europe, a crossing point between Asia and Europe and important path of various arboviruses, utilizing the Ion Torrent next-generation sequencing (NGS) platform and a custom bioinformatics pipeline based on TRINITY assembler and alignment algorithms. The analysis of the RNA virome of 10 Culicoides species resulted in the identification of the genomic signatures of 14 novel RNA viruses, including three fully assembled viruses and four segmented viruses with at least one segment fully assembled, most of which were significantly divergent from previously identified related viruses from the Solemoviridae, Phasmaviridae, Phenuiviridae, Reoviridae, Chuviridae, Partitiviridae, Orthomyxoviridae, Rhabdoviridae, and Flaviviridae families. Each Culicoides species carried a species-specific set of viruses, some of which are related to viruses from other insect vectors in the same area, contributing to the idea of a virus-carrier web within the ecosystem. The identified viruses not only expand our current knowledge on the virome of Culicoides but also set the basis of the genetic diversity of such viruses in the area of southeastern Europe. Furthermore, our study highlights that such metagenomic approaches should include as many species as possible of the local virus-carrier web that interact and share the virome of a geographical area.

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Section: Discussionmentioning

confidence: 99%

Dissecting the Species-Specific Virome in Culicoides of Thrace

et al. 2022

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“…Although ISVs cannot grow in mammalian cell lines, a study in Brazil isolated a novel insectspecific virus, Guapiaçu virus (GUAPV), from the plasma sample of a febrile person 9 .…”

Section: Discussionmentioning

confidence: 99%

“…During active yellow fever virus outbreaks, mosquito samples were trapped across nine (9) states in Nigeria between 2017 and 2020 for metagenomic sequencing. Generally, three trapping methods were used in this study; egg, larval and adult collections.…”

Section: Sample Descriptionmentioning

confidence: 99%

“…Additionally, an increasing number of viruses specific to arthropods, classified as insect-specific viruses (ISVs), have been identified in the last two decades in diverse mosquito populations around the world [2][3][4][5][6][7][8] . ISVs and pathogenic arboviruses evolutionary relationship remains uncertain 9 . However, evolution from arthropodspecific viruses has been assumed for the genus Flavivirus.…”

Section: Introductionmentioning

confidence: 99%

“…In the course of various YFV outbreaks in Nigeria between 2017 and 2020, we collected vector samples (mostly Aedes spp) in sites where there were active YFV cases. Next-generation sequencing (NGS) was carried out on 26 pools of 1,300 mosquitoes (50 mosquitoes per pool) across nine (9) states in Nigeria using a metagenomic protocol as previously described 14 . In this paper we present our findings and discuss the implications.…”

Section: Introductionmentioning

confidence: 99%

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Metagenomic Sequencing Characterizes a Wide Diversity of Viruses in Field Mosquito Samples in Nigeria

Oguzie

Nwangwu²,

Oluniyi

et al. 2021

Preprint

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Mosquito vectors are a tremendous public health threat as one in six diseases worldwide is vector-borne transmitted mainly by mosquitoes. In the last couple of years, there have been active Yellow fever virus (YFV) outbreaks in many settings in Nigeria, and nationwide, entomological surveillance has been a significant effort geared towards understanding these outbreaks. In this study, we used a metagenomic sequencing approach to characterize viruses present in vector samples collected during various outbreaks of Yellow fever (YF) in Nigeria between 2017 and 2020. Mosquito samples were grouped into pools of one to fifty mosquitoes, each based on species, sex and location. Twenty-five pools of Aedes spp and one pool of Anopheles spp collected from nine states were sequenced and metagenomic analysis was carried out. We identified a wide diversity of viruses belonging to various families in this sample set. Seven different viruses detected included: Fako virus, Phasi Charoen-like virus, Verdadero virus, Chaq like-virus, Aedes aegypti totivirus, cell fusing agent virus and Tesano Aedes virus. Although there are no reports of these viruses being pathogenic, they are an understudied group in the same families and closely related to known pathogenic arboviruses. Our work shows the revolutionary power of genomics to help detect the presence of viruses in mosquito vectors and unravel their pathogenic potential. This can help develop diagnostics and vaccines to help prevent the diseases caused by these viruses before they cause outbreaks in human populations.

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The microbial RNA metagenome of Aedes albopictus (Diptera: Culicidae) from Germany

et al. 2022

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Aedes albopictus is a highly invasive mosquito species that has become widespread across the globe. In addition, it is an efficient vector of numerous pathogens of medical and veterinary importance, including dengue, chikungunya and Zika viruses. Among others, the vector potential of mosquitoes is influenced by their microbiome. However, this influence is very dynamic and can vary between individuals and life stages. To obtain a rough overview on the microbiome of Ae. albopictus populations in Germany, pooled female and pooled male individuals from seven German locations were investigated by total RNA sequencing. The mosquito specimens had been collected as larvae in the field and processed immediately after adult emergence, i.e. without females having fed on blood. RNA fragments with high degrees of identity to a large number of viruses and microorganisms were identified, including, for example, Wolbachia pipientis and Acinetobacter baumannii, with differences between male and female mosquitoes. Knowledge about the natural occurrence of microorganisms in mosquitoes may be translated into new approaches to vector control, for example W. pipientis can be exploited to manipulate mosquito reproduction and vector competence. The study results show how diverse the microbiome of Ae. albopictus can be, and the more so needs to be adequately analysed and interpreted.

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Guapiaçu virus, a new insect-specific flavivirus isolated from two species of Aedes mosquitoes from Brazil

Cited by 14 publications

References 64 publications

Dissecting the Species-Specific Virome in Culicoides of Thrace

Dissecting the Species-Specific Virome in Culicoides of Thrace

Metagenomic Sequencing Characterizes a Wide Diversity of Viruses in Field Mosquito Samples in Nigeria

The microbial RNA metagenome of Aedes albopictus (Diptera: Culicidae) from Germany

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