Guidance on Statistical Reporting to Help Improve Your Chances of a Favorable Statistical Review

Harhay, Michael O.; Donaldson, Gavin C.

doi:10.1164/rccm.202003-0477ed

Cited by 12 publications

(5 citation statements)

References 19 publications

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“…Methodological details are available in the online data supplement. Study design, hypothesis, methods, analyses, and findings are reported according to the STROBE checklist for cross-sectional studies [16, 17].…”

Section: Methodsmentioning

confidence: 99%

Increased expression of ACE2, the SARS-CoV-2 entry receptor, in alveolar and bronchial epithelium of smokers and COPD subjects

Jacobs

Eeckhoutte

Wijnant

et al. 2020

Preprint

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Rationale: Smokers and patients with chronic obstructive pulmonary disease (COPD) are at increased risk for severe Coronavirus Disease 2019 (COVID-19). Objectives: We investigated the expression of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) entry receptor ACE2 and the protease TMPRSS2 in lung tissue from never smokers and smokers with and without COPD. Methods: In a cross-sectional, observational study we measured mRNA expression of ACE2 and TMPRSS2 by RT-PCR in lung tissue samples from 120 well phenotyped subjects. Next, protein levels of ACE2 were visualized by immunohistochemistry on paraffin sections from 87 subjects and quantified in alveolar and bronchial epithelium. Finally, primary human bronchial epithelial cells (HBECs) were cultured at air liquid interface and exposed to air or cigarette smoke. Results: ACE2 mRNA expression was significantly higher in lung tissue from current smokers and subjects with moderate to very severe COPD and correlated with physiological parameters of airway obstruction and emphysema. Pulmonary expression levels of TMPRSS2 were significantly higher in patients with (very) severe COPD and correlated significantly with ACE2 expression. Importantly, protein levels of ACE2 were elevated in both alveolar and bronchial epithelium of current smokers and subjects with moderate to very severe COPD. Finally, TMPRSS2 mRNA expression increased in in vitro cultured HBECs upon acute exposure to cigarette smoke. Conclusions: We demonstrate increased expression of ACE2 in lungs of smokers and COPD subjects, which might facilitate host cell entry of SARS-CoV-2. These findings help identifying populations at risk for severe COVID-19.

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Section: Methodsmentioning

confidence: 99%

Increased expression of ACE2, the SARS-CoV-2 entry receptor, in alveolar and bronchial epithelium of smokers and COPD subjects

Jacobs

Eeckhoutte

Wijnant

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…4,5 Indeed, epidemiologists have spent decades thinking about causality and developing methodologies to establish causal inference, from the well-known Bradford Hill criteria 6 to the Rubin potential outcomes framework 7 to directed acyclic graphs, 8 a method of visualizing causal pathways so as to help determine the appropriate statistical analysis. The epidemiological literature also includes critiques of methods for causal inference, for instance, urging more cautious application of propensity scores 9 or advising against the use of E values, a statistic purported to give an estimate of confounding. 10 In light of these considerations, we are introducing a set of guidelines for papers published in our urology journals.…”

mentioning

confidence: 99%

Guidelines for Reporting Observational Research in Urology: The Importance of Clear Reference to Causality

et al. 2023

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“…Indeed, epidemiologists have spent decades thinking about causality and developing methodologies to establish causal inference, from the well‐known Bradford Hill criteria [6] to the Rubin potential outcomes framework [7] to directed acyclic graphs [8], a method of visualising causal pathways so as to help determine the appropriate statistical analysis. The epidemiological literature also includes critiques of methods for causal inference, e.g., urging more cautious application of propensity scores [9] or advising against the use of E‐values, a statistic purported to give an estimate of confounding [10].…”

Section: Scenario Language To Use Language To Avoidmentioning

confidence: 99%

Guidelines for reporting observational research in urology: the importance of clear reference to causality

et al. 2023

View full text Add to dashboard Cite

Guidance on Statistical Reporting to Help Improve Your Chances of a Favorable Statistical Review

Cited by 12 publications

References 19 publications

Increased expression of ACE2, the SARS-CoV-2 entry receptor, in alveolar and bronchial epithelium of smokers and COPD subjects

Increased expression of ACE2, the SARS-CoV-2 entry receptor, in alveolar and bronchial epithelium of smokers and COPD subjects

Guidelines for Reporting Observational Research in Urology: The Importance of Clear Reference to Causality

Guidelines for reporting observational research in urology: the importance of clear reference to causality

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