Guided bone regeneration using a hydrophilic membrane made of unsintered hydroxyapatite and poly(L-lactic acid) in a rat bone-defect model

Ikumi, Reo; Miyahara, Takashige; Akino, Norio; Tachikawa, Noriko; Kasugai, Shohei

doi:10.4012/dmj.2017-385

Cited by 17 publications

(22 citation statements)

References 29 publications

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“…The results from the PLLA group were in agreement with those reported by Amano and colleagues [34]; i.e., the bone regeneration only occurred in the space created by the PLLA membrane, which could be explained by the guided bone regeneration (GBR) principle with the PLLA sheet acting as a barrier [34,35]. On the other hand, in the u-HA/PLLA group, the bone regeneration phenomenon may be due to a similar GBR mechanism [36]. Moroi et al published a study in 2013 using either a u-HA/PLLA or titanium mesh as the GBR membrane to reconstruct a rabbit mandibular defect [37].…”

Section: Discussionsupporting

confidence: 89%

Bone Regeneration Potential of Uncalcined and Unsintered Hydroxyapatite/Poly l-lactide Bioactive/Osteoconductive Sheet Used for Maxillofacial Reconstructive Surgery: An In Vivo Study

et al. 2019

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Uncalcined and unsintered hydroxyapatite/poly l-lactide (u-HA/PLLA) material has osteoconductive characteristics and is available for use as a maxillofacial osteosynthetic reconstruction device. However, its bone regeneration ability in the maxillofacial region has not been fully investigated. This study is the first to assess the bone regenerative potential of osteoconductive u-HA/PLLA material when it is used for repairing maxillofacial bone defects. A total of 21 Sprague-Dawley male rats were divided into three groups—the u-HA/PLLA, PLLA, or sham control groups. A critical size defect of 4 mm was created in the mandible of each rat. Then, the defect was covered with either a u-HA/PLLA or PLLA sheet on the buccal side. The rats in each group were sacrificed at 2, 4, or 8 weeks. The rats’ mandibles were sampled for histological analysis with hematoxylin and eosin staining, histomorphometry, and immunohistochemistry with Runx2 and osteocalcin (OCN) antibody. The amount of newly formed bone in the u-HA/PLLA group was significantly higher than that of the PLLA group. The expression of Runx2 and OCN in the u-HA/PLLA group was also significantly higher. These results demonstrate that the u-HA/PLLA material has excellent bone regenerative ability and confirm its applicability as a reconstructive device in maxillofacial surgery.

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Section: Discussionsupporting

confidence: 89%

Bone Regeneration Potential of Uncalcined and Unsintered Hydroxyapatite/Poly l-lactide Bioactive/Osteoconductive Sheet Used for Maxillofacial Reconstructive Surgery: An In Vivo Study

et al. 2019

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“…In addition, Dong et al [ 51 ] demonstrated that the amount of newly formed bone in a rat model was significantly greater in the u-HA/PLLA group than in the PLLA group. The osteoconductive features of u-HA/PLLA were also demonstrated in an in vivo study by Ikumi [ 128 ]. Critical bone defects (8 mm in diameter) were surgically created on the parietal bones of rats and covered by u-HA/PLLA or collagen membranes.…”

Section: U-ha/plla An Outstanding Bioresorbable Compositementioning

confidence: 72%

A Narrative Review of u-HA/PLLA, a Bioactive Resorbable Reconstruction Material: Applications in Oral and Maxillofacial Surgery

et al. 2021

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The advent of bioresorbable materials to overcome limitations and replace traditional bone-reconstruction titanium-plate systems for bone fixation, thus achieving greater efficiency and safety in medical and dental applications, has ushered in a new era in biomaterial development. Because of its bioactive osteoconductive ability and biocompatibility, the forged composite of uncalcined/unsintered hydroxyapatite and poly L-lactic acid (u-HA/PLLA) has attracted considerable interest from researchers in bone tissue engineering, as well as from clinicians, particularly for applications in maxillofacial reconstructive surgery. Thus, various in vitro studies, in vivo studies, and clinical trials have been conducted to investigate the feasibility and weaknesses of this biomaterial in oral and maxillofacial surgery. Various technical improvements have been proposed to optimize its advantages and limit its disadvantages. This narrative review presents an up-to-date, comprehensive review of u-HA/PLLA, a bioactive osteoconductive and bioresorbable bone-reconstruction and -fixation material, in the context of oral and maxillofacial surgery, notably maxillofacial trauma, orthognathic surgery, and maxillofacial reconstruction. It simultaneously introduces new trends in the development of bioresorbable materials that could used in this field. Various studies have shown the superiority of u-HA/PLLA, a third-generation bioresorbable biomaterial with high mechanical strength, biocompatibility, and bioactive osteoconductivity, compared to other bioresorbable materials. Future developments may focus on controlling its bioactivity and biodegradation rate and enhancing its mechanical strength.

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“…When using various replacement materials, the details of bone defect restoration depend both on the material itself and on the experimental model: the type of animal, the localization of the experimental bone defect, etc. [ 21 – 23 ]. As a rule, histological studies of interaction between materials and bone tissue are intended to assess inflammatory reactions, severity of vascularization, formation, and maturation of new bone taking into account the area around the implants [ 24 ].…”

Section: Resultsmentioning

confidence: 99%

Biological Response to a Novel Hybrid Polyoligomer: in vitro and in vivo Models

Bokov¹,

Bulkin²,

Davydenko³

et al. 2020

Sovrem Tehnol Med

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The aim of the study is to evaluate biocompatibility of a novel hybrid polyoligomer in in vitro and in vivo models. Materials and Methods. Cytotoxicity of the material was investigated using the MTT assay with human dermal fibroblasts as test cultures. To study direct interaction of the hybrid polyoligomer with cells, the fibroblasts were cultured on the polymer samples for 96 h, the cultures were assessed every 24 h using fluorescence microscopy. To study the tissue reaction in the area of contact with the donor bed and the morphological features of the implanted sample restructuring, a case-control study was performed using a rabbit model. Samples of hybrid polyoligomer were implanted into the bone defect formed in the left iliac crest in 10 rabbits. In the control group, the prepared allograft samples were transplanted into similar defects in 10 animals. The rabbits were sacrificed 4 and 8 weeks after the operation. The standard morphological methods with hematoxylin and eosin staining and immunohistochemical Ki-67 proliferation marker evaluation were used to assess the state of tissues in the defect area. Results. The results demonstrate that the hybrid polyoligomer is not cytotoxic (cytotoxicity score 0–1), cells adhere well to its surface, retain their viability and typical morphology throughout the entire observation period. No negative impact of material implantation on the health state and behavior of animals was detected. Morphological examination showed the absence of inflammatory changes, formation of thin-walled capillary vessels, and considerable proliferative activity of mesenchymal cells in the defect area, even though it was more intense than in the control group. Conclusion. No inflammation signs were detected by 8 th week of the experiment. It was defined that new bone was beginning to form. The results of analysis support the conclusion that the developed hybrid materials are prospective for further research as potential bone substitute.

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Guided bone regeneration using a hydrophilic membrane made of unsintered hydroxyapatite and poly(L-lactic acid) in a rat bone-defect model

Cited by 17 publications

References 29 publications

Bone Regeneration Potential of Uncalcined and Unsintered Hydroxyapatite/Poly l-lactide Bioactive/Osteoconductive Sheet Used for Maxillofacial Reconstructive Surgery: An In Vivo Study

Bone Regeneration Potential of Uncalcined and Unsintered Hydroxyapatite/Poly l-lactide Bioactive/Osteoconductive Sheet Used for Maxillofacial Reconstructive Surgery: An In Vivo Study

A Narrative Review of u-HA/PLLA, a Bioactive Resorbable Reconstruction Material: Applications in Oral and Maxillofacial Surgery

Biological Response to a Novel Hybrid Polyoligomer: in vitro and in vivo Models

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