1993
DOI: 10.1002/cyto.990140703
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Guideline for flow cytometric immunophenotyping: A report from the national institute of allergy and infectious diseases, division of AIDS

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Cited by 192 publications
(130 citation statements)
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“…The diagnosis of asthma and reactive airways disease reported by parents and recorded by WITS medical personnel in the first 1-2 years life of the study subjects suggested an overuse of the diagnosis of asthma as applied to wheezing infants with respiratory syncytial virus or rhinovirus present with asthma-like symptoms (data not shown). 17,[34][35][36] Many of these young infants infected with respiratory viruses, particularly those with underlying allergies, indeed go on to have persistent asthma, but a majority have their symptoms remit in a few years. The WITS program did not provide a long-term evaluation of the persistence of asthma symptoms with measurement of pulmonary function.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…The diagnosis of asthma and reactive airways disease reported by parents and recorded by WITS medical personnel in the first 1-2 years life of the study subjects suggested an overuse of the diagnosis of asthma as applied to wheezing infants with respiratory syncytial virus or rhinovirus present with asthma-like symptoms (data not shown). 17,[34][35][36] Many of these young infants infected with respiratory viruses, particularly those with underlying allergies, indeed go on to have persistent asthma, but a majority have their symptoms remit in a few years. The WITS program did not provide a long-term evaluation of the persistence of asthma symptoms with measurement of pulmonary function.…”
Section: Discussionmentioning
confidence: 99%
“…16 For flow cytometry, samples were prepared for staining and analysis by means of whole-blood lysis, as previously published. 17 The diagnosis of HIV infection required two positive assays, HIV culture or HIV DNA PCR. Two-to-three negative HIV assays after 1 month of age were required for the assignment of a child to the HIV− group.…”
Section: Laboratory Studiesmentioning
confidence: 99%
“…For dual-color flow cytometric analysis, samples were prepared by whole-blood lysis [31]. The same reagent lots were used at all sites (Becton-Dickinson) and consisted of an isotype control (IgG1 and IgG2a), CD45/CD14 (gating reagent), CD4/ CD8 (compensation reagent), CD3/CD4 (CD4 + T lymphocytes), CD3/CD8 (CD8 + T lymphocytes), CD8/CD38 (activated CD8 + T lymphocytes), CD8/HLA-DR (activated CD8 + T lymphocytes), CD3 Ϫ /CD16 + CD56 + (natural killer cells), and CD3 Ϫ / CD19 (B lymphocytes), conjugated to fluorescein isothiocyanate and phycoerythrin, respectively [32].…”
Section: Methodsmentioning
confidence: 99%
“…CD4+ cell counts were performed using standard flow cytometric methods in laboratories certified by the DAIDS Quality Assurance program [7]. Progesterone levels were measured at each visit using an enzyme-modified immunoassay .…”
Section: Methodsmentioning
confidence: 99%