Guidelines for establishing a 3-D printing biofabrication laboratory

Sanicola, Henry W.; Stewart, Caleb; Mueller, Michael; Ahmadi, Farzad; Wang, Dadong; Powell, Sean K.; Sarkar, Korak; Cutbush, Kenneth; Woodruff, Maria A.; Brafman, David A.

doi:10.1016/j.biotechadv.2020.107652

Cited by 16 publications

(4 citation statements)

References 141 publications

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“…Bioink printability can be semi-quantified based on the perimeter and area of square holes, which are calculated via ImageJ software using microscope images [50]. Satisfactory printability largely depends on intrinsic features of the applied bioink, such as surface tension, mechanical characteristics, viscosity, and cross-linking mechanisms [21,51]. The mechanical characteristics of bioink can be measured by a specific value of Young's modulus or compression modulus.…”

Section: Strategies For Improving Decm Bioink Printabilitymentioning

confidence: 99%

Strategies for improving the 3D printability of decellularized extracellular matrix bioink

Zhang¹,

Wang²,

Zheng³

et al. 2023

Theranostics

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3D bioprinting is a revolutionary technology capable of replicating native tissue and organ microenvironments by precisely placing cells into 3D structures using bioinks. However, acquiring the ideal bioink to manufacture biomimetic constructs is challenging. A natural extracellular matrix (ECM) is an organ-specific material that provides physical, chemical, biological, and mechanical cues that are hard to mimic using a small number of components. Organ-derived decellularized ECM (dECM) bioink is revolutionary and has optimal biomimetic properties. However, dECM is always "non-printable" owing to its poor mechanical properties. Recent studies have focused on strategies to improve the 3D printability of dECM bioink. In this review, we highlight the decellularization methods and procedures used to produce these bioinks, effective methods to improve their printability, and recent advances in tissue regeneration using dECM-based bioinks. Finally, we discuss the challenges associated with manufacturing dECM bioinks and their potential large-scale applications.

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Section: Strategies For Improving Decm Bioink Printabilitymentioning

confidence: 99%

Strategies for improving the 3D printability of decellularized extracellular matrix bioink

Zhang¹,

Wang²,

Zheng³

et al. 2023

Theranostics

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“…Previous results indicate that such electrical stimulation can improve alignment, elongation, and proliferation of cells. 111 The grade of innovation and the complexity, which is characteristic of biofabrication technologies and the resulting novel devices and products generated with them, need to struggle with the natural opposition in some sections of the market. Such classic skepticism underlines the importance of generating effective public engagement protocols to get industry and end-users involved first in understanding the need for such complexity and, then, to help on pushing these technologies toward societal acceptance and use.…”

Section: Future Directions In Standardization and Scalable Productionmentioning

confidence: 99%

“…The grade of innovation and the complexity, which is characteristic of biofabrication technologies and the resulting novel devices and products generated with them, need to struggle with the natural opposition in some sections of the market. Such classic skepticism underlines the importance of generating effective public engagement protocols to get industry and end‐users involved first in understanding the need for such complexity and, then, to help on pushing these technologies toward societal acceptance and use 112 . In this direction, ethical considerations need to focus on ensuring the sustainability of biomaterials, cell sourcing, and biofabrication of tumor models and making sure the benefits are universally available.…”

Section: Future Directions In Standardization and Scalable Productionmentioning

confidence: 99%

Biofabrication approaches and regulatory framework of metastatic tumor‐on‐a‐chip models for precision oncology

Nieto

Jiménez

Moroni

et al. 2022

Medicinal Research Reviews

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The complexity of the tumor microenvironment (TME) together with the development of the metastatic process are the main reasons for the failure of conventional anticancer treatment. In recent years, there is an increasing need to advance toward advanced in vitro models of cancer mimicking TME and simulating metastasis to understand the associated mechanisms that are still unknown, and to be able to develop personalized therapy. In this review, the commonly used alternatives and latest advances in biofabrication of tumor‐on‐chips, which allow the generation of the most sophisticated and optimized models for recapitulating the tumor process, are presented. In addition, the advances that have allowed these new models in the area of metastasis, cancer stem cells, and angiogenesis are summarized, as well as the recent integration of multiorgan‐on‐a‐chip systems to recapitulate natural metastasis and pharmacological screening against it. We also analyze, for the first time in the literature, the normative and regulatory framework in which these models could potentially be found, as well as the requirements and processes that must be fulfilled to be commercially implemented as in vitro study model. Moreover, we are focused on the possible regulatory pathways for their clinical application in precision medicine and decision making through the generation of personalized models with patient samples. In conclusion, this review highlights the synergistic combination of three‐dimensional bioprinting systems with the novel tumor/metastasis/multiorgan‐on‐a‐chip systems to generate models for both basic research and clinical applications to have devices useful for personalized oncology.

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“…Excellent printability refers to the capacity of a bioink to support precise 3D bioprinting of a living-cell-laden construct with pre-designed structures [73]. Achieving satisfactory printability is highly dependent on the properties of applied bioinks, bioprinter configurations, and biofabrication strategies [74,75]. The dECM bioink should possess an appropriate viscosity that enables tissue fabrication using 3D bioprinting technology, as analyzed in Section 2.…”

Section: Printabilitymentioning

confidence: 99%

Tissue-Specific Decellularized Extracellular Matrix Bioinks for Musculoskeletal Tissue Regeneration and Modeling Using 3D Bioprinting Technology

Park

Gao

Cho

2021

IJMS

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The musculoskeletal system is a vital body system that protects internal organs, supports locomotion, and maintains homeostatic function. Unfortunately, musculoskeletal disorders are the leading cause of disability worldwide. Although implant surgeries using autografts, allografts, and xenografts have been conducted, several adverse effects, including donor site morbidity and immunoreaction, exist. To overcome these limitations, various biomedical engineering approaches have been proposed based on an understanding of the complexity of human musculoskeletal tissue. In this review, the leading edge of musculoskeletal tissue engineering using 3D bioprinting technology and musculoskeletal tissue-derived decellularized extracellular matrix bioink is described. In particular, studies on in vivo regeneration and in vitro modeling of musculoskeletal tissue have been focused on. Lastly, the current breakthroughs, limitations, and future perspectives are described.

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Guidelines for establishing a 3-D printing biofabrication laboratory

Cited by 16 publications

References 141 publications

Strategies for improving the 3D printability of decellularized extracellular matrix bioink

Strategies for improving the 3D printability of decellularized extracellular matrix bioink

Biofabrication approaches and regulatory framework of metastatic tumor‐on‐a‐chip models for precision oncology

Tissue-Specific Decellularized Extracellular Matrix Bioinks for Musculoskeletal Tissue Regeneration and Modeling Using 3D Bioprinting Technology

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