Guidelines for Manufacturing and Application of Organoids: Brain

Abstract

This study offers a comprehensive overview of brain organoids for researchers. It combines expert opinions with technical summaries on organoid definitions, characteristics, culture methods, and quality control. This approach aims to enhance the utilization of brain organoids in research. Brain organoids, as three-dimensional human cell models mimicking the nervous system, hold immense promise for studying the human brain. They offer advantages over traditional methods, replicating anatomical structures, physiological features, and complex neuronal networks. Additionally, brain organoids can model nervous system development and interactions between cell types and the microenvironment. By providing a foundation for utilizing the most human-relevant tissue models, this work empowers researchers to overcome limitations of two-dimensional cultures and conduct advanced disease modeling research.

Standards for Organoids

“…This issue of the International Journal of Stem Cells includes special reports based on the OSI guidelines. It features the latest information on standard organ-specific production and explores the future development directions: Ahn et al ( 6 ), introduces cell type-specific culture strategies for organoids and presents quantitative and qualitative testing methods at endpoints; Lee et al ( 7 ), discusses factors critical to organoid viability, including cell and organoid packing and transportation methods; Moon et al ( 8 ), provides up-to-date information on culturing conditions and procedures for hepatotoxicity evaluation organoids, liver-specific functionalities, essential cell compositions, and test methods for quality evaluation criteria; Lee et al ( 9 ), for heart; Kang et al ( 10 ), for kidney; Lim et al ( 11 ), for lung; Kwak et al ( 12 ), for brain; and Lee et al ( 13 ), for skin. In addition to the above guidelines, Son et al ( 14 ) established the standardized manufacturing, maturation, and quality assessment protocols for human intestinal organoids through OSI activites.…”