Guidelines for the laboratory diagnosis of genital herpes in eastern European countries

Domeika, Marius; Bashmakova, М. A.; Savicheva, Alevtina; Kolomiec, N; Sokolovskiy, E. V.; Hallén, A; Unemo, Magnus; Ballard, Ronald C.

doi:10.2807/ese.15.44.19703-en

Cited by 18 publications

(11 citation statements)

References 42 publications

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“…In order to improve the present situation, consensus guidelines aimed at optimizing the laboratory diagnosis of HSV infections in the region have been developed (12) and, it is hoped, will soon be adopted and implemented in the EE SRH countries.…”

Section: Discussionmentioning

confidence: 99%

Survey of Diagnostic Services for Genital Herpes in Fourteen Countries in Eastern Europe

Domeika¹,

Babayan²,

Ismailov³

et al. 2011

Acta Derm Venerol

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This paper reports survey-based data on the diagnosis and management of genital herpes simplex virus (HSV) infection in 14 countries of the Eastern European Network for Sexual and Reproductive Health (EE SRH). Only 43% of the countries could provide the number of genital HSV cases recorded at national level. Eighty-six percent of countries employed syndromic management in cases of genital ulcer disease. Most countries performed type-specific and/or non-type-specific enzyme immunoassays to detect HSV antibodies. Non-type-specific serology for diagnostic purposes should be actively discouraged. Direct detection methods for HSV, such as PCR, antigen detection and culture, are available in the region, but their usage was extremely low. Their use in Eastern European countries should be actively promoted. The availability of laboratory services must be improved, and countries in the region should implement consensus recommendations for the laboratory diagnosis of genital HSV infections in order to improve clinical practice.

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Section: Discussionmentioning

confidence: 99%

Survey of Diagnostic Services for Genital Herpes in Fourteen Countries in Eastern Europe

Domeika¹,

Babayan²,

Ismailov³

et al. 2011

Acta Derm Venerol

Self Cite

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“…With regard to different guidelines for laboratory diagnosis of genital herpes, for patients with symptoms, detection of viral DNA by nucleic acid amplification tests (NAAT) is most appropriate. Virus isolation on cell cultures can also be performed (34).…”

Section: Diagnosismentioning

confidence: 99%

“…For populations at high risk of HSV infection, serological testing is recommended. The best practice is using a combination of type-specific serologic testing and direct virus-detection methods in order to differentiate the type of infecting virus as well as primary and recurrent infections (33,34). Because of their low sensitivity and specificity, cytological examination using conventional staining procedures (Tzanck smears, the Papanicolaou test, or Romanowsky stains) are not recommended for reliable diagnosis of HSV infections (33)(34)(35).…”

Section: Diagnosismentioning

confidence: 99%

Laboratory diagnosis and epidemiology of herpes simplex 1 and 2 genital infections

Biškup

Uršič

Petrovec

2015

Acta Dermatovenerologica Alpina Pannonica Et Adriatica

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Herpes simplex virus types 1 and 2 are the main cause of genital ulcers worldwide. Although herpes simplex virus type 2 is the major cause of genital lesions, herpes simplex virus type 1 accounts for half of new cases in developed countries. Herpes simplex virus type 2 seroprevalence rises with sexual activity from adolescence through adulthood. Slovenian data in a high-risk population shows 16% seroprevalence of HSV-2. HSV-1 and HSV-2 DNA in genital swabs was detected in 19% and 20.7%, respectively. In most cases, genital herpes is asymptomatic. Primary genital infection with herpes simplex virus types 1 and 2 can be manifested by a severe clinical picture, involving the vesicular skin and mucosal changes and ulcerative lesions of the vulva, vagina, and cervix in women and in the genital region in men. Direct methods of viral genome detection are recommended in the acute stage of primary and recurrent infections when manifest ulcers or lesions are evident. Serological testing is recommended as an aid in diagnosing genital herpes in patients with reinfection in atypical or already healed lesions. When herpes lesions are present, all sexual activities should be avoided to prevent transmission of infection. Antiviral drugs can reduce viral shedding and thus reduce the risk of sexual transmission of the virus.

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“…Because of well-developed culture protocols and a lack of FDA-cleared PCR tests, viral culture remains an important method of diagnosis for many laboratories, and maintenance of viral culture capabilities by clinical laboratories is still recommended by some groups (28,29). Additionally, the emergence of resistance to first-line antivirals, such as acyclovir, underscores the necessity to maintain viral culture capabilities.…”

Section: Methods For Detection Of Hsvmentioning

confidence: 99%

Light Microscopy, Culture, Molecular, and Serologic Methods for Detection of Herpes Simplex Virus

2014

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Herpes simplex virus 1 (HSV-1) and 2 (HSV-2) cause a variety of human diseases, ranging from acute to chronic and mild to severe. The absence of curative therapy results in lifelong carriage marked by recurrent outbreaks and allows transmission of the virus to uninfected individuals. Nonspecific lesions, variable presentation, and chronic carriage necessitate the use of different laboratory testing methods appropriate for each presentation. A thorough understanding of the performance characteristics and limitations of available tests is critical for selection of the appropriate test and interpretation of results. Clinical sensitivity, specificity, and selection of the appropriate methodology is paramount to avoid misdiagnosis and guide therapy. In this article we review the different methods for detection and typing of HSV, including light microscopy, culture, serology, and nucleic acid-based tests. We discuss the strengths and weaknesses of each method for diagnosing HSV infection, cite performance characteristics, and review appropriate clinical uses.

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Guidelines for the laboratory diagnosis of genital herpes in eastern European countries

Cited by 18 publications

References 42 publications

Survey of Diagnostic Services for Genital Herpes in Fourteen Countries in Eastern Europe

Survey of Diagnostic Services for Genital Herpes in Fourteen Countries in Eastern Europe

Laboratory diagnosis and epidemiology of herpes simplex 1 and 2 genital infections

Light Microscopy, Culture, Molecular, and Serologic Methods for Detection of Herpes Simplex Virus

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