Guidelines for the Use of Fishes in Research

Connecting post-release mortality to the physiological stress response of large coastal sharks in a commercial longline fishery

“…These float packages were hydrodynamic, approximately 3 x 7 x 12 cm in size, and weighed 125 g in air (70 g positively buoyant in seawater, see [ 54 ]). This amount of positive buoyancy represents less than 0.5% of the body weight of tagged sharks, and is thus well below the 2% of animal body weight typically recommended for tagging studies [ 55 , 56 ]. Float packages were attached to the first dorsal fin of sharks at two points using a tether made from plastic cable ties or monofilament with a built-in galvanic timed release (International Fishing Devices Inc., Northland, New Zealand), which corrodes in seawater after a predetermined number of days ( Fig 1 ), in this case ~1–5.…”

Effect of anticoagulants on farmed giant kokopu, Galaxias argenteus (Gmelin 1789) haematological parameters and erythrocyte fragility

“…Sampling was conducted within the farm under permit number FW141 for commercial operations and practice. Fish were handled and euthanized according to the American Society for Fishes' Guidelines of the Use of Fishes in Research (2014) and reported following the ARRIVE Guidelines for Animal Research (Kilkenny, Browne, Cuthill, Emerson, & Altman, 2010). Eight hundred microlitres of blood were immediately drawn by caudal vein puncture using plain 3 ml syringes attached to a 20 gauge Terumo needle.…”

Fluid‐preserved fishes are one solution for assessing historical change in fish trophic level

“…Our study strictly followed the currently used preservation protocols of natural history collections (e.g., Jenkins et al, 2014), but these practices have evolved over time, and curators have not generally documented their preservation practices. Our work and that of other preservation studies cannot predict the variable effects of these preservation protocol differences.…”

“…The purpose of this study was to test whether CSIA-AA-N can be used to generate reliable trophic-level estimates for fish that have been fixed using the fluid-preservation method most commonly employed in natural history collections (e.g., Jenkins et al, 2014). We sought to understand whether the estimated trophic positions of fluid-preserved specimens differed from pre-fixation samples and to understand how this varied across time as specimens were passed through the preservation process.…”