Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

Abstract

The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples with examples of flow cytometry applications in the context of autoimmune diseases, cancers as well as acute and chronic infectious diseases. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer-reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state-of-the-art handbook for basic and clinical researchers.

Clinical and immunological features of SARS-CoV-2 breakthrough infections in vaccinated individuals requiring hospitalization

“…Samples were acquired on a BD LSR II flow cytometer (BD Biosciences) and analysed with FlowJo v10 software. All flow cytometric analyses were performed following published guidelines (Cossarizza et al, 2021).…”

“…Full gating strategy is reported in Supplemental Figure 7. Flow cytometry experiments were performed using published guidelines (Cossarizza et al, 2021)…”

Plasma Cytokine Atlas Reveals the Importance of TH2 Polarization and Interferons in Predicting COVID-19 Severity and Survival

“…Since measuring an extended number of cytokines represents the best strategy to investigate complex pathologic conditions ( 17 ), the aim of our study was to evaluate the prognostic value of an extensive set of 62 plasma biomarkers measured at hospital admission to predict the risk of mechanical ventilation or death by day 28.…”

Protocol for generating human immune system mice and hydrodynamic injection to analyze human hematopoiesis in vivo

“… Acquire the samples to collect the desired volume of sample using available flow cytometer. Note: For detailed guidelines and description of flow cytometer set-up when performing flow cytometry experiments please refer to ( Cossarizza et al., 2021 ; Prosser et al., 2021 ; Rico et al., 2021 ) Analyze FCS files using flow cytometry analysis software. We routinely use NovoExpress Software or FlowJo for flow cytometer data analysis (a gating strategy is shown in Figure 4 ).…”

“…Note: For detailed guidelines and description of flow cytometer set-up when performing flow cytometry experiments please refer to ( Cossarizza et al., 2021 ; Prosser et al., 2021 ; Rico et al., 2021 )…”