Guiding mesenchymal stem cell differentiation using mesoporous silica nanoparticle-based films

Andrée, Lea; Barata, David; Sutthavas, Pichaporn; Habibović, Pamela; Rijt, Sabine van

doi:10.1016/j.actbio.2019.07.008

Cited by 24 publications

(22 citation statements)

References 41 publications

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“…Of note, the procedure did not alter the chemical properties of the neat and functionalized surfaces; therefore, the NPs deposition differed only for the chemical functionalization. These results agree with the work of Andrée L. and co-authors that used mesoporous NPs with different characteristics to obtain a substrate for evaluating the effect on mesenchymal stem cell differentiation [16]. Hence, our model allows the evaluation of the hBM-MSCs and hASC proliferation, shape, adhesion, and differentiation.…”

Section: Discussionsupporting

confidence: 90%

“…In fact, stem cells are able to collect the chemical-physical cues of biomaterials and transduce them in intracellular signaling that may influence the cell shape and function [7,22,35,36,[49][50][51]86]. In this regard, some research groups have demonstrated that mesoporous NPs may influence cell behavior or stem cell differentiation through their nanoscale structure, functionalization, and delivery system [16,22,87,88].…”

Section: Discussionmentioning

confidence: 99%

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Functionalized Silica Star-Shaped Nanoparticles and Human Mesenchymal Stem Cells: An In Vitro Model

et al. 2021

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The biomedical translational applications of functionalized nanoparticles require comprehensive studies on their effect on human stem cells. Here, we have tested neat star-shaped mesoporous silica nanoparticles (s-MSN) and their chemically functionalized derivates; we examined nanoparticles (NPs) with similar dimensions but different surface chemistry, due to the amino groups grafted on silica nanoparticles (s-MSN-NH2), and gold nanoseeds chemically adsorbed on silica nanoparticles (s-MSN-Au). The different samples were dropped on glass coverslips to obtain a homogeneous deposition differing only for NPs’ chemical functionalization and suitable for long-term culture of human Bone Marrow–Mesenchymal stem cells (hBM-MSCs) and Adipose stem cells (hASCs). Our model allowed us to demonstrate that hBM-MSCs and hASCs have comparable growth curves, viability, and canonical Vinculin Focal adhesion spots on functionalized s-MSN-NH2 and s-MSN-Au as on neat s-MSN and control systems, but also to show morphological changes on all NP types compared to the control counterparts. The new shape was stem-cell-specific and was maintained on all types of NPs. Compared to the other NPs, s-MSN-Au exerted a small genotoxic effect on both stem cell types, which, however, did not affect the stem cell behavior, likely due to a peculiar stem cell metabolic restoration response.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Functionalized Silica Star-Shaped Nanoparticles and Human Mesenchymal Stem Cells: An In Vitro Model

et al. 2021

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“…Cells intend to bind at sharp edges of TiO 2 -NTs walls, 48 and the changed cellular morphology usually indicates the direction of cell differentiation. 49 The SEM images (Figure 2A) and phalloidin/DAPI staining ( Figure 2B) showed that macrophages that adhered to various surfaces exhibited different morphology. Macrophages cultured on the surface of Ti and TiO 2 -NTs formed a more rounded shape, which indicated the differentiation of the M1 phenotype.…”

Section: Cell Morphology and Proliferationmentioning

confidence: 98%

<p>Improved Immunoregulation of Ultra-Low-Dose Silver Nanoparticle-Loaded TiO<sub>2</sub> Nanotubes via M2 Macrophage Polarization by Regulating GLUT1 and Autophagy</p>

Chen¹,

Guan²,

Ren³

et al. 2020

IJN

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Introduction: The bone regeneration of endosseous implanted biomaterials is often impaired by the host immune response, especially macrophage-related inflammation which plays an important role in the bone healing process. Thus, it is a promising strategy to design an osteo-immunomodulatory biomaterial to take advantage of the macrophage-related immune response and improve the osseointegration performance of the implant. Methods: In this study, we developed an antibacterial silver nanoparticle-loaded TiO 2 nanotubes (Ag@TiO 2-NTs) using an electrochemical anodization method to make the surface modification and investigated the influences of Ag@TiO 2-NTs on the macrophage polarization, osteo-immune microenvironment as well as its potential molecular mechanisms in vitro and in vivo. Results: The results showed that Ag@TiO 2-NTs with controlled releasing of ultra-low-dose Ag + ions had the excellent ability to induce the macrophage polarization towards the M2 phenotype and create a suitable osteo-immune microenvironment in vitro, via inhibiting PI3K/Akt, suppressing the downstream effector GLUT1, and activating autophagy. Moreover, Ag@TiO 2-NTs surface could improve bone formation, suppress inflammation, and promote osteo-immune microenvironment compared to the TiO 2-NTs and polished Ti surfaces in vivo. These findings suggested that Ag@TiO 2-NTs with controlled releasing of ultra-low-dose Ag + ions could not only inhibit the inflammation process but also promote the bone healing by inducing healing-associated M2 polarization. Discussion: Using this surface modification strategy to modulate the macrophage-related immune response, rather than prevent the host response, maybe a promising strategy for implant surgeries in the future.

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“…osteogenic lineage. These films have been loaded with dexamethasone, a glucocorticoid known to induce osteogenic differentiation of MSCs in vitro [126]. Temporally controlled dexamethasone delivery led to increased ALP levels and matrix mineralization compared to directly supplementing dexamethasone to the medium.…”

Section: Addition Of Drugs Relevant For Bone Tissue Homeostasismentioning

confidence: 99%

Cutting Edge Endogenous Promoting and Exogenous Driven Strategies for Bone Regeneration

Macías

Alcorta-Sevillano

Infante

et al. 2021

IJMS

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Bone damage leading to bone loss can arise from a wide range of causes, including those intrinsic to individuals such as infections or diseases with metabolic (diabetes), genetic (osteogenesis imperfecta), and/or age-related (osteoporosis) etiology, or extrinsic ones coming from external insults such as trauma or surgery. Although bone tissue has an intrinsic capacity of self-repair, large bone defects often require anabolic treatments targeting bone formation process and/or bone grafts, aiming to restore bone loss. The current bone surrogates used for clinical purposes are autologous, allogeneic, or xenogeneic bone grafts, which although effective imply a number of limitations: the need to remove bone from another location in the case of autologous transplants and the possibility of an immune rejection when using allogeneic or xenogeneic grafts. To overcome these limitations, cutting edge therapies for skeletal regeneration of bone defects are currently under extensive research with promising results; such as those boosting endogenous bone regeneration, by the stimulation of host cells, or the ones driven exogenously with scaffolds, biomolecules, and mesenchymal stem cells as key players of bone healing process.

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Guiding mesenchymal stem cell differentiation using mesoporous silica nanoparticle-based films

Cited by 24 publications

References 41 publications

Functionalized Silica Star-Shaped Nanoparticles and Human Mesenchymal Stem Cells: An In Vitro Model

Functionalized Silica Star-Shaped Nanoparticles and Human Mesenchymal Stem Cells: An In Vitro Model

<p>Improved Immunoregulation of Ultra-Low-Dose Silver Nanoparticle-Loaded TiO<sub>2</sub> Nanotubes via M2 Macrophage Polarization by Regulating GLUT1 and Autophagy</p>

Cutting Edge Endogenous Promoting and Exogenous Driven Strategies for Bone Regeneration

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