Guiding Principles for Human and Animal Research During Pharmaceutical Product Development

Maheshwari, Rahul; Gupta, Aashu; Ganeshpurkar, Aditya; Chourasiya, Yashu; Tekade, Muktika; Tekade, Rakesh K.

doi:10.1016/b978-0-12-814421-3.00018-x

Cited by 6 publications

(3 citation statements)

References 102 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Intermittent noise may also have an impact on how well drugs work and how well animals procreate. [6] Odor While some animal scents are repulsive to humans, others have been shown to have profound effects on the physiology and pharmacology of experimental animals. By keeping things clean and having enough air, you can reduce the amount of odor that comes from microorganisms decomposing excreta in an animal facility.…”

Section: Noisementioning

confidence: 99%

Laboratory animal handling techniques, basic facilities and care: A review

Jadhao¹,

Lagad²,

Ingole³

2022

World J. Bio. Pharm. Health Sci.

View full text Add to dashboard Cite

Animals have been utilized in pharmaceutical scientific research and development for more than 300 years. Pharmaceutical items are a must in order to treat illnesses caused by viruses, bacteria, or homeostatic imbalance, among other causes. Pharmaceuticals must be thoroughly characterized via in vitro, in vivo, and clinical trial investigations in order to be used in humans. It is essential to quantify organism's in vivo using animals whose physiology and genetics are most similar to those of humans. Common animals used for laboratory experimental research are Cats, Rats, Dogs, Mice, Guinea pigs and Monkeys. Handling of animals includes transportation, animal housing, temperature of cages, sanitation, water, and food options etc. Proper animal handling techniques affects the outcome of research study, so it very imperative to study and to practice the basics of animal handling techniques. This review is the small efforts to compile the scientific literature and basic information regarding laboratory animal handling techniques, basic facility and care.

show abstract

Section: Noisementioning

confidence: 99%

Laboratory animal handling techniques, basic facilities and care: A review

Jadhao¹,

Lagad²,

Ingole³

2022

World J. Bio. Pharm. Health Sci.

View full text Add to dashboard Cite

show abstract

“…Multi-Organ-on-a-Chip as a Novel In Vitro Platform for GBA Research Animal models have been mainly used in GBA research [18]. However, they require experienced handling [98] and show poor experimental reproducibility [19], while real-time analysis of their responses is challenging [99]. Furthermore, extrapolation of animal data to humans can be problematic, and detailed studies into the underlying mechanisms can often be difficult due to physiological discrepancies between mammalian species [13,100].…”

Section: Gba-on-a-chip For Research On Evs In Gut Microenvironmentmentioning

confidence: 99%

Organ-on-a-Chip for Studying Gut-Brain Interaction Mediated by Extracellular Vesicles in the Gut Microenvironment

Kim

Noort

Sung

et al. 2021

IJMS

View full text Add to dashboard Cite

Extracellular vesicles (EVs) are a group of membrane vesicles that play important roles in cell-to-cell and interspecies/interkingdom communications by modulating the pathophysiological conditions of recipient cells. Recent evidence has implied their potential roles in the gut–brain axis (GBA), which is a complex bidirectional communication system between the gut environment and brain pathophysiology. Despite the evidence, the roles of EVs in the gut microenvironment in the GBA are less highlighted. Moreover, there are critical challenges in the current GBA models and analyzing techniques for EVs, which may hinder the research. Currently, advances in organ-on-a-chip (OOC) technologies have provided a promising solution. Here, we review the potential effects of EVs occurring in the gut environment on brain physiology and behavior and discuss how to apply OOCs to research the GBA mediated by EVs in the gut microenvironment.

show abstract

“…Due to its complexity, studies on GBA have mainly relied on in vivo animal models (Raimondi et al, 2020). They require experienced animal handling (Maheshwari et al, 2018), feature poor experimental reproducibility (Voelkl et al, 2020), and real-time sensing of responses is difficult (Zhang, Korolj, Lai, & Radisic, 2018). Moreover, extrapolation of animal data to humans could be problematic, leading to an increased need for in vitro experimental model for GBA research.…”

Section: Introductionmentioning

confidence: 99%

A Gut-Brain Axis-on-a-Chip for studying transport across epithelial and endothelial barriers

Kim

Sung

2021

Preprint

View full text Add to dashboard Cite

Recent research on Gut-Brain Axis (GBA) has suggested that the gut luminal environment, including the dietary components and commensal microbiota, could affect behavior, emotion, and cognitive abilities in the brain. The research on GBA has heavily relied on animal models, which makes the research challenging. Recent advances in organ-on-a-chip technology could be a solution for GBA research. In present work, we developed a modular microfluidic chip, where gut epithelial and brain endothelial cells were co-cultured to form the gut epithelial barrier and the Blood-Brain Barrier (BBB). Cell responses to microbial byproducts were examined by TEER measurement for each barrier, and we observed the transport of fluorescently labeled exosome across the gut barrier towards the BBB. Our results suggest this model can be used as a novel in vitro model of GBA for studying the interaction between the gut and the brain.

show abstract

Guiding Principles for Human and Animal Research During Pharmaceutical Product Development

Cited by 6 publications

References 102 publications

Laboratory animal handling techniques, basic facilities and care: A review

Laboratory animal handling techniques, basic facilities and care: A review

Organ-on-a-Chip for Studying Gut-Brain Interaction Mediated by Extracellular Vesicles in the Gut Microenvironment

A Gut-Brain Axis-on-a-Chip for studying transport across epithelial and endothelial barriers

Contact Info

Product

Resources

About