Guiding principles for identification, evaluation and conservation of Vitis vinifera L. subsp. sylvestris

Zdunić, Goran; Maul, Erika; Dias, J. E. E.; Organero, Gregorio Muñoz; Carka, F.; Maletić, Edi; Savvides, Savvas; Jahnke, Gizella; Nagy, Zóra Annamária; Nikolić, D.; Ivanišević, Dragan; Beleski, Klime; Maraš, Vladislav; Mugoša, Milena; Kodžulović, V.; Radić, Tomislav; Hančević, Katarina; Mucalo, Ana; Lukšić, Katarina; Butorac, Lukrecija; Maggioni, L.; Schneider, Anna; Schreiber, Toni; Lacombe, Thierry

doi:10.5073/vitis.2017.56.127-131

Cited by 2 publications

(2 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nevertheless, there are groups that have used custom combinations of SSR markers to support their claims ( Supplementary Table 1 ). The utilization of SSR markers has been expanded to include wild Vitis accessions like V. sylvestris or V. sativa ( Zdunic et al., 2017 ). The first six OIV markers (VVS2, VVMD5, VVMD7, VVMD27, VrZAG62, VrZAG79) were evaluated under the EU Project GENRES081 ( This and Dettweiler, 2003 ) and have been proved suitable for grapevine variety characterization due to their ability to produce high degree of allelic polymorphisms (13-23 alleles per locus) and establishing high discriminatory power ( Jung et al., 2008 ).…”

Section: Most Commonly Used Genetic Markers: Simple Sequence Repeatsmentioning

confidence: 99%

Vitis vinifera genotyping toolbox to highlight diversity and germplasm identification

et al. 2023

View full text Add to dashboard Cite

The contribution of vine cultivation to human welfare as well as the stimulation of basic social and cultural features of civilization has been great. The wide temporal and regional distribution created a wide array of genetic variants that have been used as propagating material to promote cultivation. Information on the origin and relationships among cultivars is of great interest from a phylogenetics and biotechnology perspective. Fingerprinting and exploration of the complicated genetic background of varieties may contribute to future breeding programs. In this review, we present the most frequently used molecular markers, which have been used on Vitis germplasm. We discuss the scientific progress that led to the new strategies being implemented utilizing state-of-the-art next generation sequencing technologies. Additionally, we attempted to delimit the discussion on the algorithms used in phylogenetic analyses and differentiation of grape varieties. Lastly, the contribution of epigenetics is highlighted to tackle future roadmaps for breeding and exploitation of Vitis germplasm. The latter will remain in the top of the edge for future breeding and cultivation and the molecular tools presented herein, will serve as a reference point in the challenging years to come.

show abstract

Section: Most Commonly Used Genetic Markers: Simple Sequence Repeatsmentioning

confidence: 99%

Vitis vinifera genotyping toolbox to highlight diversity and germplasm identification

et al. 2023

View full text Add to dashboard Cite

show abstract

“…sylvestris in the case of Vitis vinifera L. ssp. sativa cultivars [57][58][59][60][61][62][63], and of wild American species, mainly Vitis rupestris, Vitis berlandieri and Vitis riparia in the case of rootstocks [64][65][66][67][68][69]. At the same time, climate change has increased the need to search for wild relatives of different origins carrying resistance genes to mildews and, more recently, black rot [70].…”

Section: The Null Allele Problem In Population Genetic Studies In Vit...mentioning

confidence: 99%

The SSR Null Allele Problem, and Its Consequences in Pedigree Reconstruction and Population Genetic Studies in Viticulture

Jahnke

Smidla

Deák³

et al. 2022

Horticulturae

View full text Add to dashboard Cite

Null alleles are alleles that are recessive to codominant markers without any effect on the phenotype. In SSR assays, there are several reasons for the lack of amplification at a locus: the primer does not bind well, longer fragments do not amplify due to imperfections in the PCR reaction, or the amount of DNA in the sample is insufficient. In microsatellite studies, null alleles are mostly used in pedigree analysis and population genetics calculations such as diversity estimation. Null alleles in pedigree analysis can cause rejection of the true parent; if not recognized while in population genetics they distort the results in underestimating diversity. In this review, the effects caused by null-alleles in viticultural research and its possible solutions were summarized.

show abstract

Guiding principles for identification, evaluation and conservation of Vitis vinifera L. subsp. sylvestris

Cited by 2 publications

References 3 publications

Vitis vinifera genotyping toolbox to highlight diversity and germplasm identification

Vitis vinifera genotyping toolbox to highlight diversity and germplasm identification

The SSR Null Allele Problem, and Its Consequences in Pedigree Reconstruction and Population Genetic Studies in Viticulture

Contact Info

Product

Resources

About