Guizhi Fuling capsule, an ancient Chinese formula, attenuates endometriosis in rats via induction of apoptosis

Hu, Chenyao; Wang, Z.; Pang, Zhonghua; Lü, Wei; Cai, Xueting; Yang, Jianrong; Wang, D.; Cao, Peng

doi:10.3109/13697137.2013.876618

Cited by 37 publications

(24 citation statements)

References 18 publications

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“…Metabonomic technology has been considered as an efficient tool for diagnosis and evaluation of TCM therapy, because its systemic features are consistent with the comprehensive therapeutic efficacies and mechanisms of TCM action (Su et al, ; Tan et al, ; Tan et al, ). GFC is a historically documented formula for prevention of PD with no adverse effects (Hu et al, ; Lan et al, ). In our study, an RP‐UPLC‐MS coupled with HILIC‐UPLC‐MS‐based metabonomics approach was used to analyze metabolite profiling before and after treatment by GFC in PD rats, as well as the possible intervention mechanism of GFC.…”

Section: Discussionmentioning

confidence: 99%

“…Wolf (Poria), Paeonia lactiflora Pall (Herbaceous peony), Paeonia suffruticosa andrews (Moutan Cortex) and Amygdalus persica L. (Persicae Semen) (Liu et al, ). GFC has been widely applied in the clinical prevention and treatment of dysmenorrheal, uterine fibroids, endometriosis, ovarian cysts and pelvic inflammatory disease, which are considered to be caused by the disharmony patterns of blood stasis in TCM (Hu et al, ; Li et al, ; Yao & Shulan, ). Our previous studies have shown that GFC could significantly inhibit the expression of cyclooxygenase‐2 and oxytocin receptor, and reduce the level of prostaglandin F 2a (PGF 2a ), intracellular Ca 2+ and NO in uterine tissue of oxytocin‐induced rats (Lan et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Intergrated metabonomic study of the effects of Guizhi Fuling capsule intervention on primary dysmenorrheal using RP‐UPLC–MS complementary with HILIC‐UPLC–MS technique

Lang

Meng

Sun

et al. 2017

Biomedical Chromatography

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Guizhi Fuling capsule (GFC), developed from the traditional Chinese prescription of Guizhi Fuling Wan, has been commonly used for the treatment of primary dysmenorrhea (PD). However, the intervention effective mechanism in vivo has not been well elucidated. In this study, an integrated plasma metabonomic strategy based on RP-UPLC-MS coupled with HILIC-UPLC-MS technique has been developed to investigate the global therapeutic effects and intervention mechanisms of GFC on dysmenorrhea rats induced by oxytocin. The 20 potential biomarkers were identified and primarily related to sphingolipid metabolism, steroid hormone biosynthesis, glycerophospholipid metabolism, amino acid metabolism, lipid metabolism and energy metabolism. The results showed that the GFC has therapeutic effects on rats with dysmenorrhea via the regulation of multiple metabolic pathways. Some new potential biomarkers associated with primary dysmenorrhea such as phenylalanine, tryptophan, taurine, carnitine, betaine, creatine and creatinine have been discovered in this study for the first time. This study provides a metabonomic platform based on RP-UPLC-MS complementary to HILIC-UPLC-MS technique to investigate both nonpolar and polar compounds, so as to get a more comprehensive metabolite information to yield insight into the pathophysiology of PD and assessing the efficacy of GFC on PD rats.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Intergrated metabonomic study of the effects of Guizhi Fuling capsule intervention on primary dysmenorrheal using RP‐UPLC–MS complementary with HILIC‐UPLC–MS technique

Lang

Meng

Sun

et al. 2017

Biomedical Chromatography

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“…It consists of five herbs (Cinnamomi ramulus, W. extensa, P. suffruticosa Andrews, P. persica, and P. lactiflora) [17,18]. GBH has been used mainly for treating primary dysmenorrhoea, uterine myoma [19], and climacteric symptoms such as hot flashes [18]. A phase II trial to assess the efficacy of GBH (TU-025) Tsumura & Co (Tokyo, Japan) for hot flash management was conducted in the United States; however, the results did not show significant clinical effectiveness.…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of Gyejibongnyeong-Hwan for shoulder pain: study protocol for a randomised, wait-list controlled pilot trial

Jang

Sung

et al. 2020

Trials

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Background: Shoulder pain is an uncomfortable feeling in the muscle around the shoulder. The cause of myalgia is the accumulation of lactic acid in muscles and impaired blood circulation, which is called blood stasis in traditional East Asian medicine. This study aimed to explore the therapeutic effect of Gyejibongnyeong-Hwan (GBH) for shoulder discomfort related to blood stasis before and after treatment.Methods/design: This study will be a double-centre, randomised, wait-list controlled pilot trial. Participants with shoulder pain and with a visual analogue scale score of 4 or higher out of 10, blood stasis score of 9 or higher, and triglyceride level of ≥150 mg/dl or total cholesterol level of ≥200 mg/dl will be recruited from two university hospitals. A total of 40 participants will be assigned to the immediate and waiting treatment groups. The immediate treatment group will receive GBH for 8 weeks on enrolment while the waiting treatment group will receive GBH for 8-16 weeks after 8 weeks of controlled waiting. The primary outcome is shoulder pain, and the secondary outcomes are the blood stasis score, blood pressure, ankle-brachial pressure index, brachial-ankle pulse wave velocity, body mass index, waist circumference, indexes of oximetry, and levels of blood lipid, blood sugar, resistin, C-reactive protein, serum amyloid P, and D-dimer. Discussion:The results of this pilot trial will be the bases for a full-scale clinical trial of GBH.

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“…Hu et al reported that GZFLC attenuated endometriosis in rats via induction of apoptosis and inhibition of cell proliferation and metastasis [19]. In terms of its anticancer effect, this phenomenon was also found in human hepatocellular carcinoma [20, 21], bladder cancer [22], and cervical cancer [23].…”

Section: Discussionmentioning

confidence: 99%

The Effects of Guizhi Fuling Capsule Drug Serum on Uterine Leiomyoma Cells and Its Mechanism

Shen

et al. 2016

Evidence-Based Complementary and Alternative Medicine

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Aims. To observe the effects of Guizhi Fuling Capsule (GZFLC) drug serum on uterine leiomyoma cells and explore its mechanism. Main Methods. Sixty Sprague Dawley rats were randomly divided into two groups (normal saline lavage group and GZFLC lavage group), then, respectively, blank serum and GZFLC drug serum were collected, and finally human uterine leiomyoma cells were treated. Human leiomyoma tissues were collected from 20 patients who underwent uterine leiomyomas operations, and leiomyoma cells were primary cultured. The leiomyoma cells were treated by GZFLC drug serum in different concentrations (10%, 20%, and 30%) and variable treatment time (12 h, 24 h, 36 h, 48 h, and 72 h). Cell proliferation was observed using CCK8 assay. Flow cytometry and Annexin V/PI were used to assay the effects of GZFLC drug serum on cell apoptosis. Western blot analysis was used to assay the effects of GZFLC drug serum on TSC2, FOXO, and 14-3-3γ expression in uterine leiomyoma cells. Key Findings. In the concentrations of 10%～30%, GZFLC drug serum could inhibit proliferation of leiomyoma cells in dose-dependent manner; at the time of 36 h, cell inhibition rate was at the peak; GZFLC drug serum could induce apoptosis of leiomyoma also in a dose-dependent manner, and apoptosis rate quickly achieved maximum at 12 h time points, and then second apoptosis peak appeared at 36 h. Compared to nontreatment group, TSC2, FOXO, and 14-3-3γ expressions in drug serum group were significantly changed after 12 h treatment. Significance. GZFLC drug serum can efficiently inhibit the proliferation and induce apoptosis of leiomyoma cells, which is related to the 14-3-3γ pathway.

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Guizhi Fuling capsule, an ancient Chinese formula, attenuates endometriosis in rats via induction of apoptosis

Abstract: These results indicate that the GZFL capsule can induce apoptosis of endometriotic cells and inhibit cell proliferation and metastasis of endometriotic cells through the mitochondrial apoptotic pathway.

Cited by 37 publications

References 18 publications

Intergrated metabonomic study of the effects of Guizhi Fuling capsule intervention on primary dysmenorrheal using RP‐UPLC–MS complementary with HILIC‐UPLC–MS technique

Intergrated metabonomic study of the effects of Guizhi Fuling capsule intervention on primary dysmenorrheal using RP‐UPLC–MS complementary with HILIC‐UPLC–MS technique

Effectiveness of Gyejibongnyeong-Hwan for shoulder pain: study protocol for a randomised, wait-list controlled pilot trial

The Effects of Guizhi Fuling Capsule Drug Serum on Uterine Leiomyoma Cells and Its Mechanism

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