GUMBOS matrices of variable hydrophobicity for matrix‐assisted laser desorption/ionization mass spectrometry

Ghafly, Hashim Al; Siraj, Noureen; Das, Susmita; Regmi, Bishnu P.; Magut, Paul K. S.; Galpothdeniya, Waduge Indika S.; Murray, Kermit K.; Warner, Isiah M.

doi:10.1002/rcm.7027

Cited by 16 publications

(11 citation statements)

References 44 publications

(106 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To overcome this drawback, H.A. Ghafly et al [61] synthesized hydrophobic and crystalline matrix i. e. aminopyrene (AP)-based GUMBOS ( Figure 3) with different counter-anions such as Cl À , NTf 2 À and ascorbate (Asc À ). Firstly, GUMBOS [AP][Cl] was synthesized by treating AP and HCl in dichloromethane under stirring conditions for three hours.…”

Section: Synthesis and Applications Of Gumbosmentioning

confidence: 99%

See 1 more Smart Citation

Recent Progress in Synthesis and Applications of Tunable Materials and Nanomaterials Based on Organic Salts

Kaur

Chopra

2020

ChemistrySelect

View full text Add to dashboard Cite

GUMBOS (group of uniform materials based on organic salts) are a new class of solid organic ionic materials that shared similar properties as that of ionic liquids, but have a wider range of melting points from 25°C to 250°C. A large number of combinations have been made to design and synthesize multifunctional GUMBOS with tuned properties that are encouraged to explore these organic materials for various applications. Further, nanomaterials can be fabricated from GUMBOS through various reliable synthetic approaches. These nanomaterials have known as ‚nanoGUMBOS' that possessed advantageous properties at the nanoscale and can be used for target applications. Besides, the size, shape, and uniformity of nanomaterials can be tuned by varying different experimental parameters, and synthetic routes. This review is divided into two parts and presents all the published data regarding GUMBOS and nanoGUMBOS. The first part enclosed the synthesis of GUMBOS as well as their potential applications in the field of analytical, optoelectronics, biology, and sensor technology. In the second part, the synthesis of nanoGUMBOS using different methods and their applications in the field of cellular imaging, optoelectronics, catalysis, enantiorecognition, and sensor technology will be highlighted.

show abstract

Section: Synthesis and Applications Of Gumbosmentioning

confidence: 99%

“…To overcome this drawback, H.A. Ghafly et al [61] . synthesized hydrophobic and crystalline matrix i. e. aminopyrene (AP)‐based GUMBOS (Figure 3) with different counter‐anions such as Cl − , NTf 2 − and ascorbate (Asc − ).…”

Section: Synthesis and Applications Of Gumbosmentioning

confidence: 99%

Recent Progress in Synthesis and Applications of Tunable Materials and Nanomaterials Based on Organic Salts

Kaur

Chopra

2020

ChemistrySelect

View full text Add to dashboard Cite

show abstract

“…However, since the development of MALDI‐MS, only few matrices such as 2,5‐dihydroxybenzoic acid (DHB) or p ‐hydroxy‐α‐cyanocinnamic acid (HCCA) have found broad use for various analyte series . Therefore, research for new MALDI matrices has gained considerable interest during the last decade . Despite these notable efforts, it is still quite difficult to predict whether a compound will exhibit matrical properties or not, so the discovery of new matrices remains mostly empirical .…”

Section: Introductionmentioning

confidence: 99%

“…8 Therefore, research for new MALDI matrices has gained considerable interest during the last decade. [9][10][11] Despite these notable efforts, it is still quite difficult to predict whether a compound will exhibit matrical properties or not, so the discovery of new matrices remains mostly empirical. 12 In this context, lichen metabolites may represent attractive candidates in the search for new matrix molecules.…”

Section: Introductionmentioning

confidence: 99%

The inherent matrix properties of lichen metabolites in matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry

Pogam

Boustié

Richomme

et al. 2017

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

Lichen metabolites exhibit interesting MALDI matrix properties, especially for medium and low molecular weight analytes. For many of the tested molecules, matrix ion formation was very limited. This proof-of-concept study paves the way for follow-up investigations to assess the matrix properties of lichen metabolites against a wider array of analytes as well as adapting experimental settings to individually optimize the performance of successfully tested candidates.

show abstract

“…In these cases, CHCA-C3 was proposed to have high affinity with protein, so that the protein analyte could be tightly embedded in the matrix crystal and subjected to effective ionization without the interference of salt or wash process. Additionally, it has been reported that the increased affinity between matrix molecules and analyte could dramatically improve the detection sensitivity in MALDI-MS [49][50][51]. Therefore, though the detailed mechanism is not yet clear, the improved MALDI-MS performance for analysis of intact protein using CHCA-C3 matrix should be generally ascribed to high laser-induced ablation ability, even-distributed matrixanalyte co-crystal, and increased affinity for protein.…”

Section: Mechanism Analysismentioning

confidence: 99%

(E)-Propyl α-Cyano-4-Hydroxyl Cinnamylate: A High Sensitive and Salt Tolerant Matrix for Intact Protein Profiling by MALDI Mass Spectrometry

Wang

Xiao

Wang

et al. 2016

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

Abstract. Low-abundance samples and salt interference are always of great challenges for the practical protein profiling by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Herein, a series of carboxyl-esterified derivatives of α-cyano-4-hydroxycinnamic acid (CHCA) were synthesized and evaluated as matrices for MALDI-MS analysis of protein. Among them, (E)-propyl α-cyano-4-hydroxyl cinnamylate (CHCA-C3) was found to exhibit excellent assay performance for intact proteins by improving the detection sensitivity 10 folds compared with the traditional matrices [i.e., super2,5-dihydroxybenzoic acid (superDHB), sinapic acid (SA), and CHCA]. In addition, CHCA-C3 was shown to have high tolerance to salts, the ion signal of myoglobin was readily detected even in the presence of urea (8 M), NH 4 HCO 3 (2 M), and KH 2 PO 4 (500 mM), meanwhile sample washability was robust. These achievements were mainly attributed to improved ablation ability and increased hydrophobicity or affinity of CHCA-C3 to proteins in comparison with hydrophilic matrixes, leading to more efficient ionization of analyte. Furthermore, direct analysis of proteins from crude egg white demonstrated that CHCA-C3 was a highly efficient matrix for the analysis of lowabundance proteins in complex biological samples. These outstanding performances indicate the tremendous potential use of CHCA-C3 in protein profiling by MALDI-MS.

show abstract

GUMBOS matrices of variable hydrophobicity for matrix‐assisted laser desorption/ionization mass spectrometry

Cited by 16 publications

References 44 publications

Recent Progress in Synthesis and Applications of Tunable Materials and Nanomaterials Based on Organic Salts

Recent Progress in Synthesis and Applications of Tunable Materials and Nanomaterials Based on Organic Salts

The inherent matrix properties of lichen metabolites in matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry

(E)-Propyl α-Cyano-4-Hydroxyl Cinnamylate: A High Sensitive and Salt Tolerant Matrix for Intact Protein Profiling by MALDI Mass Spectrometry

Contact Info

Product

Resources

About