Gut and Vagina Microbiota Associated With Estrus Return of Weaning Sows and Its Correlation With the Changes in Serum Metabolites

Zhong, Jia; Liu, Min; Ke, Shanlin; Huang, Xiaowan; Fang, Shouguo; He, Maozhang; Fu, Hao; Chen, Congying; Huang, Lusheng

doi:10.3389/fmicb.2021.690091

Cited by 15 publications

(17 citation statements)

References 68 publications

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“…A notable finding in our study was the dominance of Bacteroidetes over the Firmicutes. This was in contrast to findings from studies among other breeds of pigs [39][40][41] (Table S13). The glaring dominance of the Bacteroidetes were a peculiar feature in the JBP vaginal microbiota.…”

Section: Microbial Composition In the Vaginal Canalcontrasting

confidence: 99%

Oropharyngeal, proximal colonic, and vaginal microbiomes of healthy Korean native black pig gilts

et al. 2023

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Background Exploring the microbiome in multiple body sites of a livestock species informs approaches to promote its health and performance through efficient and sustainable modulation of these microbial ecosystems. Here, we employed 16S rRNA gene sequencing to describe the microbiome in the oropharyngeal cavity, proximal colon, and vaginal tract of Jeju Black pigs (JBP), which are native to the Korean peninsula. Results We sampled nine 7-month-old JBP gilts raised under controlled conditions. The most abundant phyla that we found within the oropharyngeal microbiota were Proteobacteria, Bacteroidetes, Fusobacteria and Firmicutes, collectively providing core features from twenty-five of their genera. We also found a proximal colonic microbial core composed of features from twenty of the genera of the two predominant phyla, Firmicutes, and Bacteroidetes. Remarkably, within the JBP vaginal microbiota, Bacteroidetes dominated at phylum level, contrary to previous reports regarding other pig breeds. Features of the JBP core vaginal microbiota, came from seventeen genera of the major phyla Bacteroidetes, Firmicutes, Proteobacteria, and Fusobacteria. Although these communities were distinct, we found some commonalities amongst them. Features from the genera Streptococcus, Prevotella, Bacillus and an unclassified genus of the family Ruminococcaceae were ubiquitous across the three body sites. Comparing oropharyngeal and proximal colonic communities, we found additional shared features from the genus Anaerorhabdus. Between oropharyngeal and vaginal ecosystems, we found other shared features from the genus Campylobacter, as well as unclassified genera from the families Fusobacteriaceae and Flavobacteriaceae. Proximal colonic and vaginal microbiota also shared features from the genera Clostridium, Lactobacillus, and an unclassified genus of Clostridiales. Conclusions Our results delineate unique and ubiquitous features within and across the oropharyngeal, proximal colonic and vaginal microbial communities in this Korean native breed of pigs. These findings provide a reference for future microbiome-focused studies and suggest a potential for modulating these communities, utilizing ubiquitous features, to enhance health and performance of the JBP.

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Section: Microbial Composition In the Vaginal Canalcontrasting

confidence: 99%

Oropharyngeal, proximal colonic, and vaginal microbiomes of healthy Korean native black pig gilts

et al. 2023

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“…Generally, the high levels of catabolism for lactating sows may lead to the greater loss of BF thickness and weight during lactation, 27 thus, carbohydrates and lipids are needed to compensate for the energy loss during sows' lactation. 28 Blood metabolites could reflect the health and metabolic status of the sows. In this study, a total of 48 differentially abundant biomarkers in the serum of sows between two groups were identified, which were mainly involved in metabolic pathways of amino acid and lipid metabolism.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Impact of dietary supplementation with N‐carbamoyl‐aspartic acid on serum metabolites and intestinal microflora of sows

et al. 2022

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Background N‐Carbamoyl‐aspartic acid (NCA) is a critical precursor for de novo biosynthesis of pyrimidine nucleotides. To investigate the cumulative effects of maternal supplementation with NCA on the productive performance, serum metabolites and intestinal microbiota of sows, 40 pregnant sows (∼day 80) were assigned into two groups: (1) the control (CON) and (2) treatment (NCA, 50 g t−1 NCA). Results Results showed that piglets from the NCA group had heavier birth weight than those in the CON group (P < 0.05). In addition, maternal supplementation with NCA decreased the backfat loss of sows during lactation (P < 0.05). Furthermore,16S‐rRNA sequencing results revealed that maternal NCA supplementation decreased the abundance of Cellulosilyticum, Fournierella, Anaerovibrio, and Oribacterium genera of sows during late pregnancy (P < 0.05). Similarly, on the 14th day of lactation, maternal supplementation with NCA reduced the diversity of fecal microbes of sows as evidenced by significantly lower observed species, Chao1, and Ace indexes, and decreased the abundance of Lachnospire, Faecalibacterium, and Anaerovorax genera, while enriched the abundance of Catenisphaera (P < 0.05). Untargeted metabolomics showed that a total of 48 differentially abundant biomarkers were identified, which were mainly involved in metabolic pathways of arginine/proline metabolism, phenylalanine/tyrosine metabolism, and fatty acid biosynthesis, etc. Conclusion Overall, the results indicated that NCA supplementation regulated intestinal microbial composition of sows and serum differential metabolites related to arginine, proline, phenylalanine, tyrosine, and fatty acids metabolism that may contribute to regulating the backfat loss of sows, and the birth weight and diarrhea rate of piglets. © 2022 Society of Chemical Industry.

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“…Interestingly, we also did find a few unique taxa that were not derived from the reproductive tract on the surface of the ovary. Some previous studies reported that intestinal microbes could enter the pelvic cavity under certain conditions (intestinal permeability change) and then colonize the ovarian tissue [ 27 , 33 ]. The intestinal microbiota could also colonize the lower reproductive tract via the anus and vagina and subsequently affect the upper reproductive tract [ 34 ].…”

Section: Origination and Association Of Ovarian Microbiota: The Footp...mentioning

confidence: 99%

Metagenomic analysis of the microbiome of the upper reproductive tract: combating ovarian cancer through predictive, preventive, and personalized medicine

Zhou

Wang

et al. 2022

EPMA Journal

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Purpose We investigated whether ovarian cancer could alter the genital microbiota in a specific way with clinical values. Furthermore, we proposed how such changes could be envisioned in a paradigm of predictive, preventive, and personalized medicine (PPPM). Methods The samples were collected using cotton swabs from the cervical, uterine cavity, fallopian tubes, and ovaries of patients subjected to the surgical procedures for the malignant/benign lesions. All samples were then analyzed by metagenomic shotgun sequencing. The distribution patterns and characteristics of the microbiota in the reproductive tract of subjects were analyzed and were interpreted in relation to the clinical outcomes of the subjects. Results While the ovarian cancer was able to alter the genital microbiota, the bacteria were the dominant microorganisms in all samples across all cohorts in the study (median 99%). The microbiota of the upper female reproductive tract were mainly from the cervical, identified by low bacterial biomass and high bacterial diversity. Ovarian cancer had a distinct microbiota signature. The tubal ligation affects its microbial distribution. There were no different species on the surface of platinum-sensitive ovarian tissues compared to samples from platinum-resistant patients. Conclusion The ovarian cancer–induced changes in microbiota magnify the potential of microbiota as a biotherapeutic modality in the treatment of ovarian cancer in this study and very likely for several malignancies and other conditions. Our findings demonstrated, for the first time, that microbiota could be dissected and applied in more specific fashion based on a predictive, preventive, and personalized medicine (PPPM) model in the treatment of ovarian cancer. Utilizing microbiota portfolio in a PPPM system in ovarian cancer would provide a unique opportunity to a clinically intelligent and novel approach in the treatment of ovarian cancer as well as several other conditions and malignancies.

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Gut and Vagina Microbiota Associated With Estrus Return of Weaning Sows and Its Correlation With the Changes in Serum Metabolites

Cited by 15 publications

References 68 publications

Oropharyngeal, proximal colonic, and vaginal microbiomes of healthy Korean native black pig gilts

Oropharyngeal, proximal colonic, and vaginal microbiomes of healthy Korean native black pig gilts

Impact of dietary supplementation with N‐carbamoyl‐aspartic acid on serum metabolites and intestinal microflora of sows

Metagenomic analysis of the microbiome of the upper reproductive tract: combating ovarian cancer through predictive, preventive, and personalized medicine

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