Gut butyrate-producing organisms correlate to Placenta Specific 8 protein: Importance to colorectal cancer progression

Huang, Chi Cheng; Shen, Ming Hung; Chen, Shao Kuan; Yang, Shung Haur; Liu, Chih Yi; Guo, Jiun‐Wen; Chang, Kang-Wei; Huang, Chin Chou

doi:10.1016/j.jare.2019.11.005

Cited by 28 publications

(32 citation statements)

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“…In the cultures of SW480 and SW620 cells with 10% supernatant from B. pullicaecorum , SW620 cell growth was reduced to 50.7% (from 359,000 cells to 182,000 cells) and SW480 cells to 77.4% (from 159,000 cells to 123,000 cells). This inhibitory effect on the CRC cell growth following butyrate treatment was also reported in our previous study ( 21 ). SLC5A8 and GPR43 expressions in butyrate-treated CRC cells were quantified ( Fig.…”

Section: Resultssupporting

confidence: 90%

“…The high signal intensities of SLC5A8 and GPR43 staining observed in the current study indicated that B. pullicaecorum administration may be associated with CRC prognosis. For instance, the present and other previous studies have observed that butyrate reduced histone deacetylase (HDAC) activity and acted as an HDAC inhibitor (21,54). SLC5A8-dependent inhibition of HDAC activity can be caused by the production of butyrate (55).…”

Section: Discussionmentioning

confidence: 58%

“…In conclusion, the present study demonstrated that the administration of B. pullicaecorum or its metabolite(s) improved the clinical outcome of CRC in a mouse model. These results indicated that B. pullicaecorum was a probiotic with anti-CRC potential (21,76).…”

Section: Discussionmentioning

confidence: 96%

“…Reverse transcription PCR. Total RNA from SW480 and SW620 cells, which were untreated or treated with 5 mM sodium butyrate (NaB) for 48 h as reported in our previous study (21) was independently extracted using a RNeasy Mini kit (Qiagen, Inc.), according to the manufacturer's protocol. Total RNA (1 µM) was reverse-transcribed to single-stranded cDNA using a high-capacity cDNA Reverse Transcription kit (cat.…”

Section: Methodsmentioning

confidence: 99%

“…Certain reports have indicated that decreased SCFA production is associated with increased CRC risk and may have applications in CRC therapy ( 20 ). Butyrate is a type of SCFA with various molecular effects on intestinal cells, including anticancer formation and cell immunity ( 21 – 23 ). SCFA transporters and receptors act as molecular links between the gut and microbes ( 24 ).…”

Section: Introductionmentioning

confidence: 99%

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A gut butyrate‑producing bacterium <em>Butyricicoccus pullicaecorum</em> regulates short‑chain fatty acid transporter and receptor to reduce the progression of 1,2‑dimethylhydrazine‑associated colorectal cancer

et al. 2020

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Gut microbes influence tumor development and progression in the intestines and may provide a novel paradigm for the treatment of colorectal cancer (CRC). Gut dysbiosis may be associated with the development and progression of CRC. Identifying the interactions between the colonic tract and gut microbiota may provide novel information relevant to CRC prevention. The present study examined the effects of butyrate-producing Butyricicoccus pullicaecorum (B. pullicaecorum) on mice with 1,2-dimethylhydrazine (DMH)-induced CRC and the microbial metabolite of B. pullicaecorum on CRC cells. Immunohistochemical staining of the mouse colon tissues and reverse transcription PCR of CRC cells were used to determine the protein and mRNA expression levels of the short-chain fatty acid (SCFA) transporter solute carrier family 5 member 8 (SLC5A8) and G-protein-coupled receptor 43 (GPR43). In CRC-bearing mice fed B. pullicaecorum, DMH-induced CRC regressed, body weight increased and serum carcinoembryonic antigen levels decreased. Notably, SLC5A8 and GPR43 were diffusely and moderately to strongly expressed in the neoplastic epithelial cells and underlying muscularis propria in the colons of the mice. In conclusion, administration of B. pullicaecorum or its metabolites improved the clinical outcome of CRC by activating the SCFA transporter and/or receptor. These results indicated that B. pullicaecorum was a probiotic with anti-CRC potential.

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Section: Resultssupporting

confidence: 90%

Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 96%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A gut butyrate‑producing bacterium <em>Butyricicoccus pullicaecorum</em> regulates short‑chain fatty acid transporter and receptor to reduce the progression of 1,2‑dimethylhydrazine‑associated colorectal cancer

et al. 2020

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Alterations in the oral and gut microbiome of colorectal cancer patients and association with host clinical factors

Wang

Zhang

Qian

et al. 2021

Intl Journal of Cancer

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Previous studies have suggested that gut microbiota plays a critical role in colorectal cancer (CRC). Although preliminary comparisons of the oral and gut microbiota between CRC and healthy control (HC) patients have been made, the association between microbiome abundance and host clinical factors has not been fully illustrated, especially oral health conditions. Matching samples of unstimulated saliva, cancer tissues or biopsies and stools were collected from 30 CRC and 30 HC patients from Shanghai Jiao Tong University affiliated Renji Hospital for 16S rRNA sequencing analysis. The diversity in salivary and mucosal microbiome, but not stool microbiome of CRC group, was significantly different from that of HC, as demonstrated by the Principal Component Analysis. Logistic regression analysis revealed that older age and higher oral hygiene index (OHI) were independent risk factors for CRC, with odds ratios and 95% confidence intervals of 1.159 (1.045‐1.284) and 4.398 (1.328‐14.567), respectively. Salivary Firmicutes to Bacteroides ratio in CRC was significantly higher than that in the HC group (P < .001), while the mucosal ratio was slightly decreased in CRC (P < .05). Salivary Rothia and Streptococcus levels were positively correlated with OHI, while Alloprevotella, Fusobacterium, Peptostreptoccus and Prevotella genera levels were negatively associated with OHI. NetShift analysis revealed that salivary Peptococcus, Centipeda and mucosal Subdoligranulum genus might act as key drivers during the process of carcinogenesis. In conclusion, the current study provides insights into the potential influence of host clinical factors on oral and gut microbiome composition and can be a guide for future studies.

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Metabiotics in Colorectal Cancer: Crosstalk Between Gut Microbiota and Host Pathology

Gulati

Singh

Kumar

et al. 2020

Probiotic Research in Therapeutics

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Gut butyrate-producing organisms correlate to Placenta Specific 8 protein: Importance to colorectal cancer progression

Cited by 28 publications

References 60 publications

A gut butyrate‑producing bacterium <em>Butyricicoccus pullicaecorum</em> regulates short‑chain fatty acid transporter and receptor to reduce the progression of 1,2‑dimethylhydrazine‑associated colorectal cancer

A gut butyrate‑producing bacterium <em>Butyricicoccus pullicaecorum</em> regulates short‑chain fatty acid transporter and receptor to reduce the progression of 1,2‑dimethylhydrazine‑associated colorectal cancer

Alterations in the oral and gut microbiome of colorectal cancer patients and association with host clinical factors

Metabiotics in Colorectal Cancer: Crosstalk Between Gut Microbiota and Host Pathology

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Gut butyrate-producing organisms correlate to Placenta Specific 8 protein: Importance to colorectal cancer progression

Cited by 28 publications

References 60 publications

A gut butyrate‑producing bacterium <em>Butyricicoccus&nbsp;pullicaecorum</em> regulates short‑chain fatty acid transporter and receptor to reduce the progression of 1,2‑dimethylhydrazine‑associated colorectal cancer

A gut butyrate‑producing bacterium <em>Butyricicoccus&nbsp;pullicaecorum</em> regulates short‑chain fatty acid transporter and receptor to reduce the progression of 1,2‑dimethylhydrazine‑associated colorectal cancer

Alterations in the oral and gut microbiome of colorectal cancer patients and association with host clinical factors

Metabiotics in Colorectal Cancer: Crosstalk Between Gut Microbiota and Host Pathology

Contact Info

Product

Resources

About

A gut butyrate‑producing bacterium <em>Butyricicoccus pullicaecorum</em> regulates short‑chain fatty acid transporter and receptor to reduce the progression of 1,2‑dimethylhydrazine‑associated colorectal cancer

A gut butyrate‑producing bacterium <em>Butyricicoccus pullicaecorum</em> regulates short‑chain fatty acid transporter and receptor to reduce the progression of 1,2‑dimethylhydrazine‑associated colorectal cancer