Gut commensals suppress interleukin-2 production through microRNA-200/BCL11B and microRNA-200/ETS-1 axes in lamina propria leukocytes of murine large intestine

Ohsaka, Fumina; Karatsu, Yugo; Kadota, Yasuhiro; Tochio, Takumi; Takemura, Nobuyuki; Sonoyama, Kei

doi:10.1016/j.bbrc.2020.10.103

Cited by 9 publications

(4 citation statements)

References 24 publications

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“…The bromodomain genes adjacent to the zinc nger domain, such as 2 B, centrosomal protein 250 kDa (CEP250), integrator complex subunit 1, transmembrane protein 199, microtubule-associated protein, RP/EB family, member 2, adaptor-related protein complex 2 mu 1 subunit, succinate dehydrogenase complex subunit C integral membrane protein 15 kDa, and tyrosyl-DNA phosphodiesterase 2 (TDP2), are predicted to be the target genes of the FXR TF 60 . Moreover, the associations of Midasin AAA ATPase 1, phosphatidylinositol transfer protein membrane associated 2 (PITPNM2), and BAF chromatin remodelling complex subunit 11b (BCL11b) with the microbiome have also been reported 61,62 . The age-dependent changes in PITPNM2, BCL11b, TDP2, and CEP250 were observed.…”

Section: Resultsmentioning

confidence: 99%

A lipidome landscape of aging in mice

Tsugawa,

Ishihara,

Ogasa

et al. 2024

Nat Aging

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Understanding the molecular mechanisms associated with aging is important to improve the longevity of healthy individuals. Herein, we performed untargeted lipidomics to elucidate the relationship between aging and lipid metabolism in 13 mouse tissues at various life stages (2, 12, 19, and 24 months), in which sex and microbiome dependencies (speci c pathogen free/germ free) were investigated. By analyzing 2,817 unique molecular pro le data of 121 lipid subclasses, we characterized the common and tissue-speci c lipidome changes associated with aging. For example, bis(monoacylglycerol)phosphate containing polyunsaturated fatty acids was enriched in various organs during aging, while other phospholipids containing saturated and monounsaturated fatty acids were decreased. In addition, we discovered an age-dependent increase of sulfonolipid (SL), which is biosynthesized in Alistipes and Odoribacter genera. SL molecules were absent in germ-free mice. Furthermore, the molecules appeared to be translocated from the intestinal lumen to various tissues such as the liver, kidney, and spleen. In the kidney, the associations of glycolipids such as galactosyl ceramides (GalCer), galabinosyl ceramides (Gal2Cer), trihexosyl ceramides (Hex3Cer), and mono and digalactosyl diacylglycerols were found in male mice, in which two lipid classes Gal2Cer and Hex3Cer were signi cantly enriched in aged mice. Integrated analysis of the kidney transcriptome revealed uridine diphosphate galactosyltransferase UGT8a, alkylglycerone phosphate synthase, and fatty acyl-CoA reductase 1 as potential enzymes responsible for male-speci c glycolipid biosynthesis in vivo, which would be relevant to sex dependency in kidney diseases. Our lipidome results will become an important resource, led to the understanding of the molecular mechanisms underlying cellular senescence and age-related diseases.

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Section: Resultsmentioning

confidence: 99%

A lipidome landscape of aging in mice

Tsugawa,

Ishihara,

Ogasa

et al. 2024

Nat Aging

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“…Recently, we compared the expression profiles of miRNAs and mRNAs in lamina propria leukocytes (LPLs) isolated from the large intestines of GF and SPF mice [ 52 ]. We employed microarray analyses with mouse miRNA and mRNA oligo chips containing 1,900 and 23,474 probes, respectively.…”

Section: Role Of Mirnas In the Modulation Of The Intestinal Immune Sy...mentioning

confidence: 99%

“…As described, comparisons between GF mice and CV or SPF mice suggested that the existence of the gut commensal microbiota influences intestinal miRNA expression [ 42 , 43 , 44 , 45 , 52 ]. We next examined whether a change in the composition of the gut commensal microbiota alters the expression of miRNAs [ 56 ].…”

Section: Role Of Mirnas In the Modulation Of The Intestinal Immune Sy...mentioning

confidence: 99%

“…Regarding our findings suggesting that gut commensal microbes promote the expression of miR-200 family members in large intestinal LPLs [ 52 ], our initial prediction was that gut microbial metabolites and/or cellular constituents might directly activate these miRNAs. However, our preliminary experiments showed that supplementation with short-chain fatty acids (SCFAs), gut microbial fermentation products of indigestible carbohydrates, failed to alter the levels of miR-200 family members in cultured LPLs isolated from the large intestines of SPF mice [ 52 ]. In addition, LPLs isolated from the large intestines of GF mice were cultured with fecal extracts prepared from the cecal contents of GF and SPF mice.…”

Section: Role Of Mirnas In the Modulation Of The Intestinal Immune Sy...mentioning

confidence: 99%

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Role of microRNAs in the crosstalk between the gut microbiota and intestinal immune system

Sonoyama

Ohsaka

2023

Bioscience of Microbiota, Food and Health

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MicroRNAs (miRNAs) are small non-coding RNA species involved in diverse physiological processes, including immunity. Accumulating evidence suggests that miRNA-induced gene silencing plays a significant role in the regulation of the intestinal immune system by the gut commensal microbiota. This review aims to provide an overview of the intestinal miRNA-mediated crosstalk between the gut microbiota and the host intestinal immune system. First, we describe the role of miRNAs in regulating the intestinal immune system. Then we describe the effect of the gut microbiota on intestinal miRNA expression. Subsequently, we describe the role of miRNAs in the modulation of the intestinal immune system by the gut microbiota. Finally, we describe the effect of host miRNAs on the gut microbiota. Although the entire picture of this complex crosstalk remains unclear, efforts to unravel it will contribute significantly to developing new strategies for preventing and treating intestinal immune disorders such as inflammatory bowel disease.

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