Gut-derived CD8<sup>+</sup> tissue-resident memory T cells are expanded in the peripheral blood and synovia of SpA patients

Guggino, Giuliana; Rizzo, Aroldo; Mauro, Daniele; Macaluso, Federica; Ciccia, Francesco

doi:10.1136/annrheumdis-2019-216456

Cited by 28 publications

(14 citation statements)

References 5 publications

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“…We were pleased to receive the correspondence of Guggino et al 1 commenting on our recent publication 2. Their studies confirm our findings of an expansion of cells with an expression profile consistent with tissue-resident memory cells (TRMs) in synovial fluid of spondyloarthritis (SpA).…”

supporting

confidence: 77%

Response to: ‘Gut-derived CD8+ tissue-resident memory T cells are expanded in the peripheral blood and synovia of SpA patients’ by Guggino et al

Qaiyum

Gracey²,

Yao³

et al. 2019

Ann Rheum Dis

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supporting

confidence: 77%

Response to: ‘Gut-derived CD8+ tissue-resident memory T cells are expanded in the peripheral blood and synovia of SpA patients’ by Guggino et al

Qaiyum

Gracey²,

Yao³

et al. 2019

Ann Rheum Dis

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“…Although TRMs do not circulate in the blood, they can sometimes be activated so that they travel within the lymphatic system [23,44,45]. A study by Guggino et al [45], assessing TRM cells in peripheral blood, gut and synovium in patients with SpA, showed that the expression of α4β7 may support the recirculation of these cells from the gut and peripheral blood to foci of inflammation in the joints. Although this study applies to the entire group of patients with ankylosing spondylitis, probably patients with PSA were also included.…”

Section: Tissue Resident Memory Cells In Psoriatic Arthritis (Psa)mentioning

confidence: 99%

Immunological Memory of Psoriatic Lesions

Owczarczyk‐Saczonek

Krajewska–Włodarczyk

Kasprowicz-Furmańczyk

et al. 2020

IJMS

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The natural course of psoriasis is the appearance of new lesions in the place of previous ones, which disappeared after a successful therapy. Recent studies of psoriasis etiopathogenesis showed that after psoriatic plaques have disappeared, in healthy skin we can still find a trace of inflammation in the form of tissue resident memory cells (TRM). They are originally responsible for protection against viral and bacterial infections in non-lymphatic tissues. In psoriatic inflammation, they are characterized by heterogeneity depending on their origin. CD8+ T cells TRM are abundantly present in psoriatic epidermis, while CD4+ TRM preferentially populate the dermis. In psoriasis, epidermal CD8+ TRM cells express CLA, CCR6, CD103 and IL-23R antigen and produce IL-17A during ex vivo stimulation. However, CD4+ CD103+ TRM can also colonize the epidermis and produce IL-22 during stimulation. Besides T cells, Th22 and epidermal DCs proved that epidermal cells in healed skin were still present and functioning after several years of disease remission. It explains the clinical phenomenon of the tendency of psoriatic lesions to relapse in the same location and it allows to develop new therapeutic strategies in the future.

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“…11 Interestingly these cells are phenotypically similar to the integrin-expressing (InEx) cells recently described in the joints in related SpA ankylosing spondylitis. 45 46 Although PsA SF T cells have the capability to produce cytokines such as IFNγ, IL-17 and GM-CSF upon in vitro stimulation, 47 we did not see any significant T cell cytokine production in our ex vivo unstimulated CyTOF assay. The ability to capture the exact moment when these cells are stimulated in vivo and exit dormancy may require a longer period of incubation or may be beyond current detection capabilities.…”

Section: Discussionmentioning

confidence: 74%

Ex vivomass cytometry analysis reveals a profound myeloid proinflammatory signature in psoriatic arthritis synovial fluid

Yager

Cole

Lara

et al. 2021

Preprint

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Objectives: A number of immune populations have been implicated in psoriatic arthritis (PsA) pathogenesis. This study used mass cytometry (CyTOF) combined with transcriptomic analysis to generate a high-dimensional dataset of matched PsA synovial fluid (SF) and blood leucocytes, with the aim of identifying cytokine production ex vivo in unstimulated lymphoid and myeloid cells. Methods: Fresh SF and paired blood were either fixed or incubated with protein transport inhibitors for 6 h. Samples were stained with two CyTOF panels: a phenotyping panel and an intracellular panel, including antibodies to both T cell and myeloid cell secreted proteins. Transcriptomic analysis by gene array of key expanded cell populations and single-cell RNA-seq, and ELISA and LEGENDplex analysis of PsA SF were also performed. Results: We observed marked changes in the myeloid compartment of PsA SF relative to blood, with expansion of intermediate monocytes, macrophages and dendritic cell populations. Classical monocytes, intermediate monocytes and macrophages spontaneously produced significant levels of the proinflammatory mediators osteopontin and CCL2 in the absence of any in vitro stimulation. By contrast minimal spontaneous cytokine production by T cells was detected. Gene expression analysis showed the genes for osteopontin and CCL2 to be amongst those most highly upregulated by PsA monocytes/macrophages; and both proteins were elevated in PsA SF. Conclusions: Using multiomic analyses we have generated a comprehensive cellular map of PsA SF and blood to reveal key expanded myeloid proinflammatory modules in PsA of potential pathogenic and therapeutic importance.

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Gut-derived CD8⁺ tissue-resident memory T cells are expanded in the peripheral blood and synovia of SpA patients

Cited by 28 publications

References 5 publications

Response to: ‘Gut-derived CD8+ tissue-resident memory T cells are expanded in the peripheral blood and synovia of SpA patients’ by Guggino et al

Response to: ‘Gut-derived CD8+ tissue-resident memory T cells are expanded in the peripheral blood and synovia of SpA patients’ by Guggino et al

Immunological Memory of Psoriatic Lesions

Ex vivomass cytometry analysis reveals a profound myeloid proinflammatory signature in psoriatic arthritis synovial fluid

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