Gut-immunity modulation in Lepidocephalichthys guntea during Aeromonas hydrophila-infection and recovery assessed with transcriptome data

Protocol to establish a disease model in Lepidocephalichthys guntea using Aeromonas hydrophila

“… In one of the tanks, expose the required number of fish for 24 h of fasting to empty the intestinal bulb. 1 Make sure you reserve a sufficient amount of water, taken straight from the master tank, for icing. To hold the fish during injection, cut a rectangular shape in the center of the soft sponge, depending on the size of the fish.…”

“…In one of the tanks, expose the required number of fish for 24 h of fasting to empty the intestinal bulb. 1 …”

“…For complete details on the use and execution of this protocol, please refer to Basak and Chakraborty. 1 …”