Gut Ischemia, Oxidative Stress, and Bacterial Translocation in Elevated Abdominal Pressure in Rats

Eleftheriadis, E; Kotzampassi, Katerina; Papanotas, K; Heliadis, N; Sarris, K.

doi:10.1007/s002689900002

Cited by 263 publications

(215 citation statements)

References 33 publications

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“…Data show that warm ischemia induces ischemiareperfusion (IR) injury and oxidative stress response of the graft. Indeed, induction of oxidative stress in association with PNP in the bowel was described [16].…”

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confidence: 99%

Renal oxidative stress following CO2 pneumoperitoneum-like conditions

et al. 2008

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Background Physiologic renal changes associated with pneumoperitoneum (PNP) have been described and various underlying mechanisms have been suggested. We investigated the possibility that PNP-associated renal damage is pressure dependent, and that oxidative stress is thereby involved. Materials and methods Seventy Wistar rat kidneys (n = 10 per group) were isolated. They were perfused with oxygenated, warm, Krebs-Henseleit solution containing 5% albumin within an isolated environment and subjected to various CO 2 pressures (0 [control], 3,5,8,12,15, and 18 mmHg) for 60 min. Half of each group was additionally perfused for 30 min at 0 mmHg pressure.Results Renal flow decreased proportionately to the applied pressure as did urine output: both decreased (P \ 0.05) after 60 and 90 min when pressure C8 mmHg was applied. Oxygen extraction decreased (P \ 0.05) during PNP in all pressurized groups. Xanthine oxidase (XO) activity and reduced glutathione in the tissues increased (P \ 0.05) proportionately to pressures C8 mmHg. All parameters slightly reversed toward baseline values, upon the release of the intra-chamber pressure, except for the 18 mmHg group's values. Conclusions CO 2 -PNP pressure induces kidney injury, possibly reversible immediately after pressure is annulled. Pressure is associated with oxidative stress, which interferes with cellular metabolism and function, possibly via an ischemic-reperfusion-like mechanism.

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confidence: 99%

Renal oxidative stress following CO2 pneumoperitoneum-like conditions

et al. 2008

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“…21 In contrast, Gong et al have measured micromolar concentrations in rat ileum after 4 h of intra abdominal hypertension at 20 mmHg and 4 h of reperfusion period. 22 Attack on proteins by free radicals leads to modifi cations in protein structure seen as an increase in protein carbonyl content.…”

Section: Discussionmentioning

confidence: 99%

Biochemical Changes in Experimental Rat Model of Abdominal Compartment Syndrome

et al. 2017

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Background: Increased intra-abdominal pressure (IAP) causes tissue ischemia, subsequent hypoxia, and impairment of normal tissue metabolism. Elevation of IAP above 20 mmHg leads to progression of abdominal compartment syndrome (ACS) that is associated with organ dysfunction or failure not previously manifested. Aim: To evaluate the eff ects of diff erent grades and time of exposure to IAP on biochemical parameters and oxidative stress in organs aff ected by ischemia using previously developed rat model. Results: Three experimental groups exposed to diff erent IAP and time frames were tested for liver, kidney, and pancreas injury by measuring the activities of tissue specifi c enzymes in blood serum. Elevated activities of aspartate aminotransferase, pancreatic amylase, lipase, and higher concentrations of D-lactate, urea, and creatinine were found in some of the experimental groups compared to a control group of animals not subjected to increased IAP. Increased levels of biomarkers of oxidative stress as well as decrease in concentration of the major cellular antioxidant glutathione indicated the presence of oxidative injury as a result of elevated IAP. Conclusions: The developed rat model is appropriate to study the mechanism and manifestation of tissue injury during diff erent grades of elevated IAP but also to test approaches aimed to attenuate the detrimental eff ects of ACS. This study also underlines the necessity of using not a single but a set of biochemical parameters in order to assess the severity of tissue injury during elevated IAP and progression to ACS. BACKGROUND

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“…Eleftheriadis et al [22] defined PN-related hepatic oxidative stress for the first time in literature [23,24], while Yılmaz et al [25] firstly demostrated PN-related renal oxidative stress in rat models.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Different Intraabdominal Pressure Protocols in Laparoscopic Procedures on Oxidative Stress Markers and Morphology in Rat Ovaries

Biler¹,

Yücebilgin²,

Şendağ³

et al. 2014

Adv Clin Exp Med

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Background. To determine the effects of different intraabdominal pressure (IAP) on the ovaries in a laparoscopic rat model. Objectives. The aim of the study was to determine the effects on the ovaries of different intraabdominal pressures (IAP) in laparoscopic surgery in a rat model. Material and Methods. Thirty-two post-pubertal nonpregnant Sprague-Dawley rats were divided randomly into four groups. In the control group, no intraabdominal pressure (IAP) was applied. In Group Pp10 and Group Pp15, an IAP of 10 and 15 mm Hg, respectively, were applied by carbon dioxide insufflation for 60 min, and a 30-min desufflation was carried out. In Group IPp15, a 15 mm Hg IAP was applied for 10 min, and then CO 2 was desufflated for 10 min. After this ischemic preconditioning, IAP was established at 15 mm Hg for 60 min, after which CO 2 was desufflated for 30 min. Erythrocyte and ovarian tissue malondialdehyde (MDA) and histopathologic damage scores were evaluated. Results. In Groups Pp10 and Pp15, ovarian tissue MDA values were significantly increased compared to the control group. In Groups Pp10 and Pp15, erythrocyte MDA values were significantly increased when compared to Group IPp15 and the control group. Ovarian histopatological assesment scores were significantly higher in Group Pp15 than in Groups Pp10 and IPp15. Conclusions. Pneumoperitoneum causes injuries to abdominal organ such as the ovaries. The ischemic preconditioning method is more effective in reducing oxidative stress due to laparoscopic pneumoperitoneum than lowpressure pneumoperitoneum methods (Adv Clin Exp Med 2014, 23, 6, 885-892).

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Gut Ischemia, Oxidative Stress, and Bacterial Translocation in Elevated Abdominal Pressure in Rats

Cited by 263 publications

References 33 publications

Renal oxidative stress following CO2 pneumoperitoneum-like conditions

Renal oxidative stress following CO2 pneumoperitoneum-like conditions

Biochemical Changes in Experimental Rat Model of Abdominal Compartment Syndrome

The Effects of Different Intraabdominal Pressure Protocols in Laparoscopic Procedures on Oxidative Stress Markers and Morphology in Rat Ovaries

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