Gut microbial profile analysis by MiSeq sequencing of pancreatic carcinoma patients in China

Ren, Zhigang; Jiang, Jianwen; Xie, Haiyang; Li, Ang; Lü, Haifeng; Xu, Shaoyan; Zhou, Lin; Zhang, Hua; Cui, Guangying; Chen, Xinhua; Liu, Yuanxing; Wu, Liming; Qin, Nan; Sun, Ranran; Wang, Wei; Wang, Weilin; Zheng, Shusen

doi:10.18632/oncotarget.18820

Cited by 169 publications

(221 citation statements)

References 54 publications

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“…Bacteroidetes, Firmicutes, Proteobacteria, and Verrucomicrobia were the top dominant populations in the three groups, together accounting for up to 95% of sequences, in which the abundance and relationship between Bacteroidetes and Firmicutes significantly contributed to gut microbial composition and changes [45,46]. Notably, we found that phylum Bacteroidetes were decreased, while phyla Proteobacteria and Verrucomicrobia Cellular Physiology and Biochemistry…”

Section: Discussionmentioning

confidence: 69%

Coreopsis Tinctoria Modulates Lipid Metabolism by Decreasing Low-Density Lipoprotein and Improving Gut Microbiota

Ren

Liu

et al. 2018

Cell Physiol Biochem

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Background/Aims: The prevalence of hyperlipidemia is increasing rapidly. The role of Coreopsis tinctoria (CT) in amending lipid metabolism in hyperlipidemia patients has not been reported. This study aims to evaluate the role of CT in altering lipid metabolism in hyperlipidemia patients and to explore the possible mechanisms mediated by gut microbiota in hyperlipidemia mice models. Methods: A retrospective analysis in 40 hyperlipidemia patients was conducted, in which 20 patients took fenofibrate and another 20 patients normatively drank water with CT. Hyperlipidemia mice models were also established. Blood biochemical tests were performed using an automatic biochemical analyzer. Liver histopathology was observed by hematoxylin and eosin staining. Ileocecal samples were collected from mice, and bacterial DNA was extracted and sequenced by MiSeq sequencing. Bacterial composition and differences were analyzed. Results: In hyperlipidemia patients, CT was associated with decreased triglyceride and low-density lipoprotein (LDL) levels without liver injury. The experimental hyperlipidemia model also verified a similar result. Gut microbial richness and diversity were significantly decreased in hyperlipidemic mice, but increased after CT treatment. Bacterial communities were significantly differentiated between normal controls and hyperlipidemic mice. CT administration improved gut microbiota composition to an approximately normal status. Meanwhile, CT administration attenuated bacterial alterations at the class, order, family, and genus levels in hyperlipidemic mice. Importantly, the genera Barnesiella, Lactobacillus, and Helicobacter achieved high discriminatory power in hyperlipidemic mice relative to normal controls. Conclusions: CT can modulate blood lipid metabolism with improvement of liver function by decreasing LDL and improving gut microbiota compositions. These findings may provide novel therapeutic strategies for patients with hyperlipidemia.

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Section: Discussionmentioning

confidence: 69%

Coreopsis Tinctoria Modulates Lipid Metabolism by Decreasing Low-Density Lipoprotein and Improving Gut Microbiota

Ren

Liu

et al. 2018

Cell Physiol Biochem

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“…Similarly, in liver cirrhosis, bacterial families like Enterobacteriaceae, Veillonellaceae and Streptococcaceae were found to be prevalent, with a decrease in beneficial populations such as Lachnospiraceae [53]. In a recent study by Ren et al, a miSeq analysis showed that some potential pathogens like Veillonella, Klebsiella, and Selenomonas and LPS-producing bacteria including Prevotella, Hallella, and Enterobacter were enriched in pancreatic adenocarcinoma, whereas probiotics including Bifidobacterium and some butyrateproducing bacteria, such as Coprococcus, Clostridium IV, Blautia, Flavonifractor and Anaerostipes were reduced [54].…”

Section: Discussionmentioning

confidence: 99%

Microbial Dysbiosis and polyamine metabolism as predictive markers for early detection of pancreatic cancer

Méndez

Kesh

Arora

et al. 2018

Preprint

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Synopsis:Gut microbiota changes during early stages of pancreatic ductal adenocarcinoma (PDAC) progression and contributes towards host polyamine pool. Both changes can be used as an early predictive marker for PDAC. Short title: Whole genome sequencing of PDAC microbiomeWord count: 4,735 Translational RelevancePancreatic carcinogenesis progresses through pre-cancerous PanIN lesions to invasive cancer. Even though these morphological changes are histologically distinct, imaging techniques are not able to distinguish the pre-invasive PanINs from normal pancreas, making detection of a tumor at a precancerous stage impossible . Thus, majority of cases (85-90%) present with advanced pancreatic cancer at the time of diagnosis. This contributes to the dismal survival rate in this disease. Our study of gut microbiome analysis on KPC mice during tumor progression followed by metabolic reconstruction and experimental validation in human samples indicate that gut-microbiome analysis along with an analysis of the microbial metabolites can be developed as potential biomarkers for detection of PDAC at early stages when histological changes are not yet grossly apparent. AbstractPurpose: The lack of tools for early detection of pancreatic ductal adenocarcinoma (PDAC) is directly correlated to the abysmal survival rate in patients. In addition to several potential detection tools under active investigation, we tested the gut microbiome and its metabolic complement as one of the earliest detection tools that could be useful in patients at high-risk for PDAC. Experimental Design:A combination of 16s pyrosequencing and whole-genome sequencing of gut microbiota was used in a spontaneous genetically engineered PDAC murine model (KRAS G12D TP53 R172H Pdx Cre or KPC). Metabolic reconstruction of microbiome was done using the HUmanN2 pipeline. Serum polyamine levels were measured from murine and patient samples using standard methods. Results:Results showed a progressive Proteobacterial and Firmicutes dominance in gut microbiota in early stages of PDAC development. Upon in silico reconstruction of active metabolic pathways within the altered microbial flora, polyamine and nucleotide biosynthetic pathways were significantly elevated.These metabolic products are known to be actively assimilated by the host and eventually utilized by rapidly dividing cells for proliferation validating their importance in the context of tumorigenesis. In KPC mice, as well as PDAC patients, we show significantly elevated serum polyamine concentration. Therefore, at the early stages of tumorigenesis, the gut microbial composition changes in a way to release metabolites that foster host tumorigenesis, thereby fulfilling the 'vicious cycle hypothesis' of the role of the microbiome in health and disease states. Conclusions:Our results provide a potential, precise, non-invasive tool for early detection of PDAC, which will result in improved outcomes.

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“…Pötgens et al demonstrated on a murine model that Klebsiella oxytoca behaves as a gut pathobiont, that is, it increases gut permeability, and thus influences cancer progression [78]. Ren et al demonstrated a significant decrease in commensal Bifidobacterium and butyrate-producing bacteria such as Coprococcus, Clostridium IV, Blautia, Flavonifractor, and Anaerostipes in PDAC patients [79]. These data suggest that enrichment in Gram-negative bacteria with a decrease in butyrate producers is positively correlated with pancreatic cancer development.…”

Section: Microflora In Pancreatic Adenocarcinomamentioning

confidence: 99%

Microbiota Alterations in Gastrointestinal Cancers

2020

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Commensal microbiota plays a critical role in the maintenance of human health. Microbes influence energy metabolism and nutrient absorption and help defend the host organism against pathogens. The composition of the gut microbiota is delicately balanced, and any alterations may lead to proinflammatory immune responses and initiation of disease processes, including cancer. Experimental evidence indicates that the human intestinal microbiota can influence tumour development and progression in the gastrointestinal tract by damaging DNA, activation of oncogenic signaling pathways, production of tumour-promoting metabolites, and suppression of the anti-tumour immune response. The aim of this article was to outline differences in human microbiota between healthy subjects and patients with gastrointestinal malignancies such as esophageal, stomach, liver, biliary tract, pancreas and colon inflammations, and cancers. A better understanding of microbiota changes in various gastrointestinal malignancies will enable a greater insight into the relationship between human microbiota composition and cancer development.

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Gut microbial profile analysis by MiSeq sequencing of pancreatic carcinoma patients in China

Cited by 169 publications

References 54 publications

Coreopsis Tinctoria Modulates Lipid Metabolism by Decreasing Low-Density Lipoprotein and Improving Gut Microbiota

Coreopsis Tinctoria Modulates Lipid Metabolism by Decreasing Low-Density Lipoprotein and Improving Gut Microbiota

Microbial Dysbiosis and polyamine metabolism as predictive markers for early detection of pancreatic cancer

Microbiota Alterations in Gastrointestinal Cancers

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