Gut microbiome alterations in high-fat-diet-fed mice are associated with antibiotic tolerance

Lv, Yuan; Yang, Kangni; Jia, Yuqian; Shi, Jingru; Tong, Ziwen; Fang, Dan; Yang, Bingqing; Su, Chengrui; Li, Ruichao; Xiao, Xia; Wang, Zhiqiang

doi:10.1038/s41564-021-00912-0

Cited by 123 publications

(93 citation statements)

References 58 publications

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“…In a separate publication, Bifidobacterium species found in breast milk were shown to produce aromatic lactic acids 15 , which may impact immune function in early life. On the other hand, high fat dietinduced alterations to gut microbiota were found to reduce antibiotic efficacy 16 in a mouse model. This was associated with a decrease in the tryptophan metabolite indole-3-acetic acid and a consequent inhibition of persister formation in vitro, highlighting the detrimental effects diet and the microbiota can have on our health.…”

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confidence: 99%

2021 in review

2021

Nat Microbiol

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confidence: 99%

2021 in review

2021

Nat Microbiol

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“…The fecal microbiota revealed that the bacterial composition in mice fed a standard diet was dominated by the Bacteroidaceae and Akkermansiaceae families but was devoid of Lachnospiraceae and Muribaculaceae. In contrast, HFD increased the numbers of bacteria belonging to the Lachnospiraceae and Muribaculaceae families, while reducing both Bacteroidaceae and Akkermansiaceae [ 38 ]. Akkermansiaceae have been found to be related to improvements in NAFLD symptoms [ 19 ], while Lachnospiraceae are related to the induction of NAFLD [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

Hepatoprotective Effects of Ixeris chinensis on Nonalcoholic Fatty Liver Disease Induced by High-Fat Diet in Mice: An Integrated Gut Microbiota and Metabolomic Analysis

Jin¹,

Cho²,

Laxi³

et al. 2022

Molecules

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Ixeris chinensis (Thunb.) Nakai (IC) is a folk medicinal herb used in Mongolian medical clinics for the treatment of hepatitis and fatty liver diseases even though its pharmacological mechanism has not been well characterized. This study investigated the hepatoprotective mechanism of IC on mice with nonalcoholic fatty liver disease (NAFLD) by integrating gut microbiota and metabolomic analysis. A high-fat diet (HFD) was used to develop nonalcoholic fatty liver disease, after which the mice were treated with oral IC (0.5, 1.5 and 3.0 g/kg) for 10 weeks. HFD induced NAFLD and the therapeutic effects were characterized by pathological and histological evaluations, and the serum indicators were analyzed by ELISA. The gut microbial and metabolite profiles were studied by 16S rRNA sequencing and untargeted metabolomic analysis, respectively. The results showed that the administration of IC resulted in significant decreases in body weight; liver index; serum biomarkers such as ALT, TG, and LDL-C; and the liver inflammatory factors IL-1β, IL-6, and TNF-α. The 16S rRNA sequencing results showed that administration of IC extract altered both the composition and abundance of the gut microbiota. Untargeted metabolomic analysis of liver samples detected a total of 212 metabolites, of which 128 were differentially expressed between the HFD and IC group. IC was found to significantly alter the levels of metabolites such as L-glutamic acid, pyridoxal, ornithine, L-aspartic acid, D-proline, and N4-acetylaminobutanal , which are involved in the regulation of glutamine and glutamate, Vitamin B6 metabolism, and arginine and proline metabolic pathways. Correlation analysis indicated that the effects of the IC extract on metabolites were associated with alterations in the abundance of Akkermansiaceae, Lachnospiraceae, and Muribaculaceae. Our study revealed that IC has a potential hepatoprotective effect in NAFLD and that its function might be linked to improvements in the composition of gut microbiota and their metabolites.

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“…Emerging evidence has implicated that HFD enhances intestinal permeability, inflammation, and disease risk ( Murphy et al, 2015 ). In mouse models, HFD induced alteration of the microbiota composition and reduced microbial diversity ( Liu et al, 2021 ). Interestingly, clinical trials associated diet-induced obesity with decrease of microbial gene richness as well as increase of low-grade inflammation ( Cotillard et al, 2013 ).…”

Section: Scfas and Bas Impact On Liver Function Inflammation And Carcinogenesismentioning

confidence: 99%

The Role of Short-Chain Fatty Acids and Bile Acids in Intestinal and Liver Function, Inflammation, and Carcinogenesis

Visekruna

Luu

2021

Front. Cell Dev. Biol.

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During the past decade, researchers have investigated the role of microbiota in health and disease. Recent findings support the hypothesis that commensal bacteria and in particular microbiota-derived metabolites have an impact on development of inflammation and carcinogenesis. Major classes of microbial-derived molecules such as short-chain fatty acids (SCFA) and secondary bile acids (BAs) were shown to have immunomodulatory potential in various autoimmune, inflammatory as well as cancerous disease models and are dependent on diet-derived substrates. The versatile mechanisms underlying both beneficial and detrimental effects of bacterial metabolites comprise diverse regulatory pathways in lymphocytes and non-immune cells including changes in the signaling, metabolic and epigenetic status of these. Consequently, SCFAs as strong modulators of immunometabolism and histone deacetylase (HDAC) inhibitors have been investigated as therapeutic agents attenuating inflammatory and autoimmune disorders. Moreover, BAs were shown to modulate the microbial composition, adaptive and innate immune response. In this review, we will discuss the recent findings in the field of microbiota-derived metabolites, especially with respect to the molecular and cellular mechanisms of SCFA and BA biology in the context of intestinal and liver diseases.

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Gut microbiome alterations in high-fat-diet-fed mice are associated with antibiotic tolerance

Cited by 123 publications

References 58 publications

2021 in review

2021 in review

Hepatoprotective Effects of Ixeris chinensis on Nonalcoholic Fatty Liver Disease Induced by High-Fat Diet in Mice: An Integrated Gut Microbiota and Metabolomic Analysis

The Role of Short-Chain Fatty Acids and Bile Acids in Intestinal and Liver Function, Inflammation, and Carcinogenesis

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