Gut Microbiome Composition and Dynamics in Hospitalized COVID-19 Patients and Patients with Post-Acute COVID-19 Syndrome

Brīvība, Monta; Silamiķele, Laila; Birzniece, Līga; Ansone, Laura; Megnis, Kaspars; Silamiķelis, Ivars; Pelcmane, Līva; Borisova, Daniella; Rozenberga, Maija; Jagare, Lauma; Elbere, Ilze; Kloviņš, Jānis

doi:10.3390/ijms25010567

Cited by 3 publications

(2 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Distinct microbial signatures were identified, with the prevalence of specific OTUs in COVID-19 patients and a particular abundance of Prevotella in severe cases. This was similar to findings from a recent study [30] which highlighted a protective role of the Prevotella genus in the long-term recovery process while other investigations [31,32] reported varied results. The variation could be attributed to various factors like sample collection methods, patient demographics, or even geographic variations in microbial populations.…”

Section: Discussionsupporting

confidence: 91%

Gut microbial profiling of COVID-19 patients in Uganda

Kateete,

Lubega,

Galiwango

et al. 2024

Preprint

View full text Add to dashboard Cite

Background:While COVID-19 spread globally, the role of the gut microbiota in patient outcomes has remained an area of exploration especially in resource limited settings. This study aimed to comprehensively profile the gut microbiome among Ugandan COVID-19 patients and infer potential implications.Methods:Nasopharyngeal swabs, stool, clinical and demographic data were collected from COVID-19 confirmed cases at the COVID-19 isolation and treatment centers in Kampala and Entebbe, Uganda, during the first and second waves of the pandemic in Uganda (i.e., 2020 and 2021, respectively). SARS-CoV-2 presence in the swab samples was confirmed by quantitative real-time RT-PCR assays. 16S rRNA metagenomic next-generation sequencing was performed on the DNA extracted from the stool samples, followed by bioinformatics analysis. Machine learning was used to determine microbes that were associated with disease severity.Results:We observed varied gut microbial composition between COVID-19 patients and healthy controls. Potentially pathogenic bacteria such as Klebsiella oxytoca, Salmonella enterica and Serratia marcescens had an increased presence in COVID-19 disease states, especially severe cases. Enrichment of opportunistic pathogens, such as Enterococcus species, and depletion of beneficial microbes, like Alphaproteobacteria, was observed between mild and severe cases. Machine learning identified age and microbes such as Ruminococcaceae, Bacilli, Enterobacteriales, porphyromonadaceae, and Prevotella copri as predictive of severity.Conclusion:These findings suggest that the microbiome plays a role in the dynamics of SARS-CoV-2 infection in African patients. The shift in abundance of specific microbes can moderately predict severity of COVID-19 in this population. Their direct or indirect roles in determining severity should be investigated further for potential therapeutic interventions.

show abstract

Section: Discussionsupporting

confidence: 91%

Gut microbial profiling of COVID-19 patients in Uganda

Kateete,

Lubega,

Galiwango

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…Nevertheless, it has been reported that there was no significant difference in the expression of bifidobacterial abundance in patients with COVID-19, with the exception of those in the ICU ( 43 ). Furthermore, no differences in probiotic abundance were found at any stage of the viral infection ( 44 ). A study conducted on a European population also demonstrated an increased expression of bifidobacterial abundance in individuals infected with SARS-CoV-2 ( 45 ).…”

Section: Discussionmentioning

confidence: 98%

Gut microbiota, inflammatory proteins and COVID-19: a Mendelian randomisation study

Chen,

Chen

2024

Front. Immunol.

View full text Add to dashboard Cite

BackgroundThe human gut microbiota has been identified as a potentially important factor influencing the development of COVID-19. It is believed that the disease primarily affects the organism through inflammatory pathways. With the aim of improving early diagnosis and targeted therapy, it is crucial to identify the specific gut microbiota associated with COVID-19 and to gain a deeper understanding of the underlying processes. The present study sought to investigate the potential causal relationship between the gut microbiota and COVID-19, and to determine the extent to which inflammatory proteins act as mediators in this relationship.MethodsBidirectional mendelian randomization (MR) and Two-step mediated MR analyses were applied to examine causative associations among 196 gut microbiota, 91 inflammatory proteins and COVID-19. The main analytical method used in the MR was the random effects inverse variance weighted (IVW) method. This was complemented by the Bayesian weighted Mendelian randomization (BWMR) method, which was utilized to test the hypothesis of MR. In order for the results to be deemed reliable, statistical significance was required for both methods. Validation was then carried out using an external dataset, and further meta-analyses were conducted to authenticate that the association was reliable.ResultsResults of our research indicated that seven gut microbiota were actively associated to the COVID-19 risk. Five inflammatory proteins were associated with COVID-19 risk, of which three were positively and two were negatively identified with COVID-19. Further validation was carried out using sensitivity analyses. Mediated MR results revealed that CCL2 was a possible mediator of causality of family Bifidobacteriaceae and order Bifidobacteriales with COVID-19, mediating at a ratio of 12.73%.ConclusionSuggesting a genetic causation between specific gut microbiota and COVID-19, our present research emphasizes the underlying mediating role of CCL2, an inflammatory factor, and contributes to a deeper understanding of the mechanism of action underlying COVID-19.

show abstract

Alterations in microbiota of patients with COVID‐19: implications for therapeutic interventions

Qiu,

Mo,

Chen

et al. 2024

MedComm

View full text Add to dashboard Cite

Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) recently caused a global pandemic, resulting in more than 702 million people being infected and over 6.9 million deaths. Patients with coronavirus disease (COVID‐19) may suffer from diarrhea, sleep disorders, depression, and even cognitive impairment, which is associated with long COVID during recovery. However, there remains no consensus on effective treatment methods. Studies have found that patients with COVID‐19 have alterations in microbiota and their metabolites, particularly in the gut, which may be involved in the regulation of immune responses. Consumption of probiotics may alleviate the discomfort caused by inflammation and oxidative stress. However, the pathophysiological process underlying the alleviation of COVID‐19‐related symptoms and complications by targeting the microbiota remains unclear. In the current study, we summarize the latest research and evidence on the COVID‐19 pandemic, together with symptoms of SARS‐CoV‐2 and vaccine use, with a focus on the relationship between microbiota alterations and COVID‐19‐related symptoms and vaccine use. This work provides evidence that probiotic‐based interventions may improve COVID‐19 symptoms by regulating gut microbiota and systemic immunity. Probiotics may also be used as adjuvants to improve vaccine efficacy.

show abstract

Gut Microbiome Composition and Dynamics in Hospitalized COVID-19 Patients and Patients with Post-Acute COVID-19 Syndrome

Cited by 3 publications

References 37 publications

Gut microbial profiling of COVID-19 patients in Uganda

Gut microbial profiling of COVID-19 patients in Uganda

Gut microbiota, inflammatory proteins and COVID-19: a Mendelian randomisation study

Alterations in microbiota of patients with COVID‐19: implications for therapeutic interventions

Contact Info

Product

Resources

About