Gut microbiome of pre-adolescent children of two ethnicities residing in three distant cities

Khine, Wei Wei Thwe; Zhang, Yuwei; Goie, Gerald Jian Yi; Wong, Mung Seong; Liong, Min‐Tze; Lee, Yeong Yeh; Cao, Hong; Lee, Yuan‐Kun

doi:10.1038/s41598-019-44369-y

Cited by 26 publications

(33 citation statements)

References 26 publications

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“…Several clinical studies on gut microbiota in intractable epilepsy reported inconsistent results, as summarized in Table 1. We presumed that the lack of consensus could be the result of inconsistency in their control over the confounding factors, such as age [23,24], body mass index [25], type of diet [26][27][28][29][30], modes of delivery [31,32], or recent antibiotic exposure [33], which are known to have an influence over the gut microbiota. Antibiotic exposure was the only variable that was consistently controlled throughout most of the previous studies.…”

Section: Discussionmentioning

confidence: 99%

Gut Bacterial Dysbiosis in Children with Intractable Epilepsy

Lee¹,

Kim²,

Shim

et al. 2020

JCM

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A few published clinical studies have evaluated the association between gut microbiota in intractable epilepsy, but with inconsistent results. We hypothesized that the factors associated with the gut bacterial composition, such as age and geography, contributed to the discrepancies. Therefore, we used a cohort that was designed to minimize the effects of possible confounding factors and compared the gut microbiota between children with intractable epilepsy and healthy controls. Eight children with intractable epilepsy aged 1 to 7 years and 32 age-matched healthy participants were included. We collected stool samples and questionnaires on their diet and bowel habits at two time points and analyzed the gut microbiota compositions. In the epilepsy group, the amount of Bacteroidetes was lower (Mann–Whitney test, false discovery rate (FDR) < 0.01) and the amount of Actinobacteria was higher (FDR < 0.01) than in the healthy group. The epilepsy subjects were 1.6- to 1.7-fold lower in microbiota richness indices (FDR < 0.01) and harbored a distinct species composition (p < 0.01) compared to the healthy controls. Species biomarkers for intractable epilepsy included the Enterococcus faecium group, Bifidobacterium longum group, and Eggerthella lenta, while the strongest functional biomarker was the ATP-binding cassette (ABC) transporter. Our study identified gut bacterial dysbiosis associated with intractable epilepsy within the cohort that was controlled for the factors that could affect the gut microbiota.

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Section: Discussionmentioning

confidence: 99%

Gut Bacterial Dysbiosis in Children with Intractable Epilepsy

Lee¹,

Kim²,

Shim

et al. 2020

JCM

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“…Chong and colleagues 10 identified that ethnicity played a major role in influencing the GM of pre-adolescents from two ethnic groups in a closely located rural setting in Malaysia. In contrast, Khine and colleagues 11 proposed that diet exerted a stronger influence on the GM than ethnicity alone in a comparison across Chinese children from Malaysia and southern China. To date, the effects of ethnicity on the GM of adult Malaysians have not been examined.…”

Section: Introductionmentioning

confidence: 89%

“…This sets Malaysia as a valuable setting to investigate the impact of ethnicity across communities. Previously, studies on the effect of ethnicity on the GM of Malaysians have resulted in differing outcomes 10 , 11 . Chong and colleagues 10 identified that ethnicity played a major role in influencing the GM of pre-adolescents from two ethnic groups in a closely located rural setting in Malaysia.…”

Section: Introductionmentioning

confidence: 99%

Ethnicity influences the gut microbiota of individuals sharing a geographical location: a cross-sectional study from a middle-income country

Dwiyanto

Hussain

Reidpath

et al. 2021

Sci Rep

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No studies have investigated the influence of ethnicity in a multi-ethnic middle-income country with a long-standing history of co-habitation. Stool samples from 214 Malaysian community members (46 Malay, 65 Chinese, 49 Indian, and 54 Jakun) were collected. The gut microbiota of the participants was investigated using 16S amplicon sequencing. Ethnicity exhibited the largest effect size across participants (PERMANOVA Pseudo-F = 4.24, R2 = 0.06, p = 0.001). Notably, the influence of ethnicity on the gut microbiota was retained even after controlling for all demographic, dietary factors and other covariates which were significantly associated with the gut microbiome (PERMANOVA Pseudo-F = 1.67, R2 = 0.02, p = 0.002). Our result suggested that lifestyle, dietary, and uncharacterized differences collectively drive the gut microbiota variation across ethnicity, making ethnicity a reliable proxy for both identified and unidentified lifestyle and dietary variation across ethnic groups from the same community.

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“…[1][2][3][4] The mature human gastrointestinal (GI) microbiome type has been categorized into two operational types, determined largely by diet, geographic location, and lifestyle: the Bacteroides-Bifidobacterium type and Prevotella type. [5][6][7][8][9] The former type is predominant among Europeans, North Americans, and Eastern Asians, 5,[9][10][11] whereas the latter is common among Southeast Asians, Mongolians, and Africans. 5,10,12 Mothers transfer their microbiomes to their children during pregnancy and delivery.…”

Section: Introductionmentioning

confidence: 99%

Indonesian children fecal microbiome from birth until weaning was different from microbiomes of their mothers

et al. 2020

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Gastrointestinal (GI) microbiota play an important role in human health and wellbeing and the first wave of gut microbes arrives mostly through vertical transmission from mother to child. This study has undertaken to understand the microbiota profile of healthy Southeast Asian mother-infant pairs. Here, we examined the fecal, vaginal and breast milk microbiota of Indonesian mothers and the fecal microbiota of their children from less than 1 month to 48 months old. To determine the immune status of children and the effect of diet at different ages, we examined the level of cytokines, bile acids in the fecal water and weaning food frequency. The fecal microbiota of the children before weaning contained mainly Bacteroides and Bifidobacterium, which presented at low abundance in the samples of mothers. After weaning, the fecal microbiome of children was mainly of the Prevotella type, with decreasing levels of Bifidobacterium, thus becoming more like the fecal microbiome of the mother. The abundance of infant fecal commensals generally correlated inversely with potential pathogens before weaning. The fecal Bifidobacterium in children correlated inversely with the consumption of complex carbohydrates and fruits after weaning. The specific cytokines related to the proliferation and maturation of immunity were found to increase after weaning. A decreasing level of primary bile acids and an increase of secondary bile acids were observed after weaning. This study highlights the change in the GI microbiota of infants to adulttype microbiota after weaning and identifies diet as a major contributing factor.

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Gut microbiome of pre-adolescent children of two ethnicities residing in three distant cities

Cited by 26 publications

References 26 publications

Gut Bacterial Dysbiosis in Children with Intractable Epilepsy

Gut Bacterial Dysbiosis in Children with Intractable Epilepsy

Ethnicity influences the gut microbiota of individuals sharing a geographical location: a cross-sectional study from a middle-income country

Indonesian children fecal microbiome from birth until weaning was different from microbiomes of their mothers

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