Gut Microbiota Comparison Between Intestinal Contents and Mucosa in Mice With Repeated Stress-Related Diarrhea Provides Novel Insight

Zhang, Chenyang; Peng, Xinxin; Shao, Haoqing; Li, Xiaoya; Wu, Yi; Tan, Zhoujin

doi:10.3389/fmicb.2021.626691

Cited by 25 publications

(24 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, microorganisms in different intestinal areas are relatively different in their composition, function, and response to external substances. For example, the intestinal mucosal microbiota is more sensitive to repeated stress-related diarrhea than the intestinal content microbiota, and more sensitive to dietary factors than the fecal microbiota [ 42 , 54 ]. Furthermore, the body lactase could be derived from the villi of intestinal mucosal epithelial cells, and the integrity of the intestinal mucosal is an important guarantee for the lactase to play the function of digestion of nutrients.…”

Section: Discussionmentioning

confidence: 99%

Lactase bacteria in intestinal mucosa are associated with diarrhea caused by high-fat and high-protein diet

Zhou

Peng²,

Deng³

et al. 2022

BMC Microbiol

View full text Add to dashboard Cite

Background Excessive fat and protein in food can cause diarrhea by disturbing the intestinal microecology. Lactase is a functional enzyme strongly associated with diarrhea, while lactase bacteria in the intestine are an important source of microbial lactase. Therefore, we reconnoiter the relationship between diarrhea induced by a high-fat and high-protein diet (HFHPD) and intestinal mucosal lactase bacteria from the perspective of functional genes. Result Operational Taxonomic Units (OTUs) were 23 and 31 in the normal group (NM) and model group (MD), respectively, and 11 of these were identical. The Chao1 and Observed specie indexes in the MD were higher than those in the NM, but this was not significant (P > 0.05). Meanwhile, the Principal coordinate analysis (PCoA) and Adonis test showed that the community structures of lactase bacteria in NM and MD were significantly different (P < 0.05). In taxonomic composition, lactase bacteria on the intestinal mucosa were sourced from Actinobacteria and Proteobacteria. Where Actinobacteria were higher in NM, and Proteobacteria were higher in MD. At the genus level, Bifidobacterium was the dominant genus (over 90% of the total). Compared to NM, the abundance of Bifidobacterium were lower in MD, while MD added sources for lactase bacteria of Rhizobium, Amycolatopsis, and Cedecea. Conclusions Our data demonstrate that HFHPD altered the community structure of lactase bacteria in the intestinal mucosa, decreased the abundance of the critical lactase bacteria, and promoted the occurrence of diarrhea.

show abstract

Section: Discussionmentioning

confidence: 99%

Lactase bacteria in intestinal mucosa are associated with diarrhea caused by high-fat and high-protein diet

Zhou

Peng²,

Deng³

et al. 2022

BMC Microbiol

View full text Add to dashboard Cite

show abstract

“…Gut microbiota is composed of LAB and MAB with different ecological niches. LAB are mainly related to metabolism, whereas MAB are closely related to immunity (Zhang et al, 2021). To comprehensively analyze the effects of SIs on the gut microbiota, we observed changes in LAB and MAB after SIs treatment.…”

Section: Discussionmentioning

confidence: 99%

Soybean isoflavones modulate gut microbiota to benefit the health weight and metabolism

Huang

Zheng

Hui

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Soybean isoflavones (SIs) are widely found in food and herbal medicines. Although the pharmacological activities of SIs have been widely reported, their effects on the intestinal microecology of normal hosts have received little attention. Five-week-old Kunming (KM) mice were administered SIs (10 mg/kg/day) for 15 days. Food intake, body weight, and digestive enzyme activity were measured. Small intestine microbiota, including lumen-associated bacteria (LAB) and mucosa-associated bacteria (MAB), were analyzed using 16S ribosomal ribonucleic acid (16S rRNA) gene sequencing. Short-chain fatty acids (SCFAs) were analyzed using gas chromatography-mass spectrometry (GC-MS). The results showed that the mice that consuming SIs showed a higher food intake but a lower body weight gain rate than that of normal mice. Sucrase, cellulase, and amylase activities reduced, while protease activity increased after SIs intervention. Moreover, SIs increased the intestinal bacterial diversity in both LAB and MAB of normal mice. The composition of LAB was more sensitive to SIs than those of MAB. Lactobacillus, Adlercreutzia, Coprococcus, Ruminococcus, Butyricicoccus, and Desulfovibrio were the differential bacteria among the LAB of mice treated with SIs. In addition, acetic acid, valeric acid, isobutyric acid, isovaleric acid, and caproic acid decreased, while butyric acid and propionic acid increased in the mice treated with SIs. Taken together, SIs are beneficial for weight control, even in short-term interventions. The specific mechanism is related to regulating the gut microbiota, changing digestive enzyme activities, and further affecting carbohydrate absorption and metabolism.

show abstract

“…Combined with our PCoA and NMDS results, a high salt diet clearly changed the structure of the gut microbiota, and intestinal bacteria may show increased growth in hypertensive animals. In fact, many recent studies have reported that increased growth of gut bacteria is common in patients with a variety of diseases ( 40 , 41 ).…”

Section: Discussionmentioning

confidence: 99%

Hypertension of liver-yang hyperactivity syndrome induced by a high salt diet by altering components of the gut microbiota associated with the glutamate/GABA-glutamine cycle

Zheng

Peng

et al. 2022

Front. Nutr.

Self Cite

View full text Add to dashboard Cite

The gut microbiota and metabolites are closely related to hypertension; however, the changes in the composition of the gut microbiome and metabolites linking a high salt diet to elevated blood pressure are not established. In this study, traditional Chinese medicine (TCM) syndrome of hypertension caused by high salt had been diagnosed and the pathogenesis of hypertension was explored from the perspective of intestinal microecology. Rats in a high salt diet-induced hypertension group (CG) and normal group (CZ) were compared by 16S rRNA gene full-length sequencing and liquid chromatography and mass spectrometry to identify differences in the bacterial community structure, metabolites, and metabolic pathways. Hypertension induced by a high salt diet belongs to liver-Yang hyperactivity syndrome. Alpha and beta diversity as well as the composition of microbiota from the phylum to species levels differed substantially between the CG and CZ groups. In an analysis of differential metabolites in the intestines, a high salt diet mainly affected the metabolism of amino acids and their derivatives; in particular, γ-aminobutyric acid (GABA) was down-regulated and glutamic acid and its derivatives were up-regulated under a high salt diet. Based on a KEGG analysis, high salt intake mainly altered pathways related to GABA and the glutamate/glutamine metabolism, such as the GABAergic synapse pathway and glutamatergic synapse pathway. The correlation analysis of differential gut microbes and differential metabolites suggested that a high salt diet promoted hypertension via the inhibition of Clostridiaceae_1 growth and alterations in the GABA metabolic pathway, leading to increased blood pressure. These findings suggest that a high salt diet induces hypertension of liver-Yang hyperactivity syndrome by mediating the microbiota associated with the glutamate/GABA-glutamine metabolic cycle via the gut–brain axis.

show abstract

Gut Microbiota Comparison Between Intestinal Contents and Mucosa in Mice With Repeated Stress-Related Diarrhea Provides Novel Insight

Cited by 25 publications

References 50 publications

Lactase bacteria in intestinal mucosa are associated with diarrhea caused by high-fat and high-protein diet

Lactase bacteria in intestinal mucosa are associated with diarrhea caused by high-fat and high-protein diet

Soybean isoflavones modulate gut microbiota to benefit the health weight and metabolism

Hypertension of liver-yang hyperactivity syndrome induced by a high salt diet by altering components of the gut microbiota associated with the glutamate/GABA-glutamine cycle

Contact Info

Product

Resources

About