Gut microbiota-dependent phenylacetylglutamine in cardiovascular disease: current knowledge and new insights

Song, Yaonan; Wei, Haoran; Zhou, Zhitong; Wang, Huiqing; Hang, Weijian; Wu, Junfang; Wang, Dao Wen

doi:10.1007/s11684-024-1055-9

Cited by 4 publications

(1 citation statement)

References 113 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the Song Y. et al experimental study it was suggested that AAAs derivates can activate platelets and mediate thrombosis via adrenergic receptor activation. Their production is connected with gut microbiota composition via a rise of pathogenic strains, such as Streptococcus spp., Enterococcus spp., and Ruminococcus spp., which can induce AF via an increase of trimethylamine-N-oxide, indole sulfate, and primary bile acids circulation [33]. The topic of thrombosis-associated metabolomics is understudied, and has a big potential for new biomarkers identification.…”

Section: Discussionmentioning

confidence: 99%

Plasma and platelets amino acids in coronary artery disease and atrial fibrillation patients – are they linked?

Melnychuk,

Sharayeva

2024

View full text Add to dashboard Cite

Introduction. The impact of circulating amino acid levels and their combinations on the pathogenesis of ischemic heart disease and atrial fibrillation is a current issue, otherwise, the platelets amino acid spectrum is still under discussion, despite the known pathogenetic role of platelets in these disorders. Aim. To compare changes in the plasma and platelets amino acid spectrum in patients with coronary artery disease and atrial fibrillation as well as to find their connections. Materials & Methods. 300 patients were divided into 3 groups: I group – 149 patients with coronary artery disease without arrhythmias, II group – 124 patients with coronary artery disease and atrial fibrillation paroxysm, and the control group – 27 patients without coronary artery disease and arrhythmias. Plasma and platelet amino acid levels were detected by means of ion exchange liquid column chromatography. Results & Conclusions. In platelets amino acid spectrum, a significant rise in leucine (12.63%), isoleucine (10.73%), and Fishers’ ratio (6.37%); a decrease in threonine (23.05%), valine (30.83%) levels, glycine (32.21%), serine (5.06%), and glycine+serine sum (20.51%) in group 2 patients was found compared with group 1, p<0.05. In the plasma amino acids spectrum, a significant increase in glutamate, branched-chain amino acids, and Fishers’ ratio and a decrease in glycine in group 2 patients was checked in comparison with group 1, p<0.05. Only 10 moderate strength correlations were revealed between the plasma and platelets amino acid spectrum of investigated patient’s groups. These changes in platelets and plasma amino acids spectrum were not significantly congruent in patients with coronary artery disease and atrial fibrillation. Plasma and platelets amino acid spectrum should be analyzed separately in patients with coronary artery disease and atrial fibrillation for further studies and evaluation of new prognostic markers and pathogenetic clues to their development. Keywords: myocardial ischemia, heart rhythm violations, proteins, metabolomics.

show abstract

Section: Discussionmentioning

confidence: 99%

Plasma and platelets amino acids in coronary artery disease and atrial fibrillation patients – are they linked?

Melnychuk,

Sharayeva

2024

View full text Add to dashboard Cite

show abstract

Unraveling SARS-CoV-2 Host-Response Heterogeneity through Longitudinal Molecular Subtyping

Wang,

Nie,

Maguire

et al. 2024

Preprint

View full text Add to dashboard Cite

Hospitalized COVID-19 patients exhibit diverse immune responses during acute infection, which are associated with a wide range of clinical outcomes. However, understanding these immune heterogeneities and their links to various clinical complications, especially long COVID, remains a challenge. In this study, we performed unsupervised subtyping of longitudinal multi-omics immunophenotyping in over 1,000 hospitalized patients, identifying two critical subtypes linked to mortality or mechanical ventilation with prolonged hospital stay and three severe subtypes associated with timely acute recovery. We confirmed that unresolved systemic inflammation and T-cell dysfunctions were hallmarks of increased severity and further distinguished patients with similar acute respiratory severity by their distinct immune profiles, which correlated with differences in demographic and clinical complications. Notably, one critical subtype (SubF) was uniquely characterized by early excessive inflammation, insufficient anticoagulation, and fatty acid dysregulation, alongside higher incidences of hematologic, cardiac, and renal complications, and an elevated risk of long COVID. Among the severe subtypes, significant differences in viral clearance and early antiviral responses were observed, with one subtype (SubC) showing strong early T-cell cytotoxicity but a poor humoral response, slower viral clearance, and greater risks of chronic organ dysfunction and long COVID. These findings provide crucial insights into the complex and context-dependent nature of COVID-19 immune responses, highlighting the importance of personalized therapeutic strategies to improve both acute and long-term outcomes.

show abstract

Threshold of PAGln Changes: Exponential Growth Between Age and Gut Microbiota in Stroke Patients

Liu,

Chu,

Wang

et al. 2024

Preprint

View full text Add to dashboard Cite

Importance PAGln, a gut microbiota-derived metabolite, is linked to increased platelet reactivity and thrombosis risk. However, its relationship with age, particularly the nonlinear patterns in ischemic stroke patients, remains unclear. Objectives To explore the nonlinear relationship between age and plasma PAGln levels in ischemic stroke patients, focusing on identifying exponential growth trends and critical age thresholds. Design, Setting, and Participants This single-center, prospective cohort study was conducted at the Department of Neurology, Minhang Hospital, Fudan University, from January 2022 to December 2023. A total of 121 patients with ischemic stroke were consecutively enrolled. Demographic information, lifestyle factors, stroke characteristics, and comorbidities were systematically collected. Plasma PAGln levels were measured using rapid resolution liquid chromatography–quadrupole time-of-flight mass spectrometry. Generalized additive models and smoothing curve fitting were applied to assess nonlinear relationships between age and PAGln levels, with threshold effect analysis used to identify age breakpoints. Multivariable regression models were applied to adjust for confounders, and subgroup analyses tested the robustness of findings. Main Outcomes and Measures Plasma PAGln levels and their association with age in ischemic stroke patients, evaluated through nonlinear models and regression analysis. Results Significant differences in PAGln levels were found across age quartiles (P=0.004), rising from 186.87 ± 95.49 µmol/L in the youngest quartile (33–54 years) to 433.11 ± 474.03 µmol/L in the oldest quartile (69–87 years). A nonlinear association between age and PAGln levels was identified (P=0.0006). Smoothing curve fitting revealed an exponential increase in PAGln levels with age. A threshold effect analysis pinpointed a breakpoint at 71 years. Below this age, no significant association between age and PAGln was observed (P=0.5394), while above 71, a significant exponential increase in PAGln levels was detected (P<0.0001). Subgroup analyses confirmed consistent results across various patient characteristics, with no significant interactions. Conclusions and Relevance A nonlinear exponential relationship exists between age and plasma PAGln levels in ischemic stroke patients, with a marked increase after 71 years. Elevated PAGln levels in elderly patients suggest significant metabolic dysregulation, potentially raising thrombosis risk. Monitoring PAGln levels in stroke patients over 71 years could provide valuable insights for personalized interventions to reduce thrombotic complications.

show abstract

Gut microbiota-dependent phenylacetylglutamine in cardiovascular disease: current knowledge and new insights

Cited by 4 publications

References 113 publications

Plasma and platelets amino acids in coronary artery disease and atrial fibrillation patients – are they linked?

Plasma and platelets amino acids in coronary artery disease and atrial fibrillation patients – are they linked?

Unraveling SARS-CoV-2 Host-Response Heterogeneity through Longitudinal Molecular Subtyping

Threshold of PAGln Changes: Exponential Growth Between Age and Gut Microbiota in Stroke Patients

Contact Info

Product

Resources

About