Gut Microbiota in Patients with Different Metabolic Statuses: Moscow Study

Abstract

The aim of this paper was to study gut microbiota composition in patients with different metabolic statuses. Methods: 92 participants aged 25–76 years (26 of whom were men), with confirmed absence of cardiovascular and other chronic diseases (but with the possible presence of cardiovascular risk factors) were included. Carotid ultrasound examinations, 16S rRNA sequencing of stool samples and diet assessments were performed. Statistical analysis was performed using R programming language, 3.1.0. Results: Enterotyping yielded two clusters differentiated by alpha-diversity. Intima-media thickness was higher in the cluster with lower diversity (adj. p < 0.001). Obesity was associated with higher Serratia (adj. p = 0.003) and Prevotella (adj. p < 0.0003) in relative abundance. Abdominal obesity was associated with higher abundance of Serratia (adj. p = 0.004) and Prevotella (adj. p = 0.0008) and lower levels of Oscillospira (adj. p = 0.0005). Glucose metabolism disturbances were associated with higher Blautia (adj. p = 0.0007) and Serratia (adj. p = 0.003) prevalence. Arterial hypertension was associated with high Blautia levels (adj. p = 0.002). The Blautia genus strongly correlated with low resistant starch consumption (adj. p = 0.007). A combination of high-fat diet and elevated Blautia levels was very common for diabetes mellitus type 2 patients (adj. p = 0.0001). Conclusion: The results show that there is a relationship between metabolic changes and higher representation of opportunistic pathogens and low diversity of gut microbiota even in apparently healthy participants.

High Oleic Acid Peanut Oil and Extra Virgin Olive Oil Supplementation Attenuate Metabolic Syndrome in Rats by Modulating the Gut Microbiota

“…Previous studies have suggested that Lachnospiraceae family is significantly increased in both obese nonalcoholic fatty liver disease and T2DM in humans [35,36]. Blautia is a genus of the family Lachnospiraceae, which was thought to take part in the development of glucose metabolism disturbances [37]. In this study, relative abundance of Lachnospiraceae and Blautia in HOPO group and EVOO group was lower than M group, but only HOPO group with significant difference.…”

<p><em>Phocea</em>, <em>Pseudoflavonifractor</em> and <em>Lactobacillus intestinalis</em>: Three Potential Biomarkers of Gut Microbiota That Affect Progression and Complications of Obesity-Induced Type 2 Diabetes Mellitus</p>

“…According to the previous reports, rich abundance Blauita is highly related to glucose disorders, low levels of insulin and arterial hypertension. 50,51 So in this case abundant Blautia and Lachnospiraceae were possibly related to cardiovascular and cerebrovascular complications. The abundance of Turicibacter significantly increases in the early stage of diabetes.…”

Dietary flaxseed oil rich in omega-3 suppresses severity of type 2 diabetes mellitus via anti-inflammation and modulating gut microbiota in rats

“…At the genus level, Blautia is a gram-positive, anaerobe bacterium belonging to the family Lachnospiraceae, which was thought to take part in the development of glucose metabolism disturbances [45]. A recent study also showed that Blautia, especially Blautiacoccoides, may activate secretion of TNF-α concentration with an even greater extent than the LPS [46].…”