Gut resistomes, microbiota and antibiotic residues in Chinese patients undergoing antibiotic administration and healthy individuals

Duan, Yujing; Chen, Zeyou; Tan, Lu; Wang, Xiaolong; Xue, Yingang; Wang, Shaopeng; Wang, Qing; Das, Ranjit; Lin, Hongtao; Hou, Jie; Li, Linyun; Mao, Daqing; Luo, Yi

doi:10.1016/j.scitotenv.2019.135674

Cited by 50 publications

(26 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Overall, a greater microbial diversity was observed in healthy controls than that of diseased subjects which are in agreement with the previous studies [34,47,55,56]. At the genus level, a high abundance of Prevotella was observed in the healthy controls than that of diseased subjects.…”

Section: Discussionsupporting

confidence: 91%

“…A number of ARGs were identified in the resistome of both healthy and diseased subjects. The presence of various ARG types in the resistome of healthy subjects is in agreement with a recent metagenomic study [34]. A metagenomic study identified various ARGs in the resistome of Yanomami Amerindian villagers whose ancestors were isolated for more than 11,000 years and have never been administered any synthetic antibiotics [35].…”

Section: Discussionsupporting

confidence: 88%

“…Their results indicated reduction in gut microbial diversity of both the treated cohorts up to the similar extent, however their gut resistome differs owing to the use of a particular group of antibiotics [1]. In addition, the higher diversity of ARGs in diseased subjects is in agreement with a recent study indicating that the overall relative abundance of various ARGs was higher significantly in patients than that of healthy controls [34]. We observed a high abundance of tetracycline and beta-lactam associated ARGs in the resistomes of both healthy and diseased subjects.…”

Section: Discussionsupporting

confidence: 79%

See 2 more Smart Citations

Fecal Microbiome and Resistome Profiling of Healthy and Diseased Pakistani Individuals Using Next-Generation Sequencing

Afridi

Chang

2021

Microorganisms

View full text Add to dashboard Cite

In this paper, we aimed to characterize the fecal microbiome and its resistomes of healthy and diseased subjects infected with multidrug-resistant Escherichia coli using next-generation sequencing (NGS). After initial screening, 26 stools samples belonging to healthy (n = 13) and diseased subjects (n = 13) were selected and subjected to NGS. A total of 23 and 42 antibiotic-resistant genes (ARGs) conferring resistance to 6 and 9 classes of antibiotics were identified in the resistomes of healthy and diseased subjects, respectively. Bacteroidetes were found to be the major phylum in both healthy and diseased subjects; however, Proteobacteria was predominantly present in the diseased subjects only. Microbial dysbiosis and predominance of various ARGs in the resistome of diseased subjects reflect the excessive usage of antibiotics in Pakistan and warrants immediate attention to regulate the use of various antimicrobials.

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 79%

See 1 more Smart Citation

Fecal Microbiome and Resistome Profiling of Healthy and Diseased Pakistani Individuals Using Next-Generation Sequencing

Afridi

Chang

2021

Microorganisms

View full text Add to dashboard Cite

show abstract

“…Numerous studies have confirmed that antibiotics have an impact on the composition and functionality of the human microbiota [104]. The impact of antibiotics on the microbiota can lead to (i) selection of resistant bacteria [105,106], (ii) domination of microbial composition by pathogenic bacteria [107], (iii) loss of bacterial diversity [108], (iv) decrease or even loss of certain bacterial species [109], (v) increase in susceptibility to infections and (vi) risk of new infection and/or recurrence.…”

Section: Drugs Interaction With Human Gut Microbiotamentioning

confidence: 99%

Gut Microbiota, Antibiotic Therapy and Antimicrobial Resistance: A Narrative Review

2020

View full text Add to dashboard Cite

Antimicrobial resistance is a major concern. Epidemiological studies have demonstrated direct relationships between antibiotic consumption and emergence/dissemination of resistant strains. Within the last decade, authors confounded spectrum activity and ecological effects and did not take into account several other factors playing important roles, such as impact on anaerobic flora, biliary elimination and sub-inhibitory concentration. The ecological impact of antibiotics on the gut microbiota by direct or indirect mechanisms reflects the breaking of the resistance barrier to colonization. To limit the impact of antibiotic therapy on gut microbiota, consideration of the spectrum of activity and route of elimination must be integrated into the decision. Various strategies to prevent (antimicrobial stewardship, action on residual antibiotics at colonic level) or cure dysbiosis (prebiotic, probiotic and fecal microbiota transplantation) have been introduced or are currently being developed.

show abstract

“…It is widely accepted that human gut microbiota is an important reservoir of ARGs [6,7]. Most of gut bacteria have a symbiotic relationship with their host, but also contain opportunistic pathogens, such as Escherichia coli and Klebsiella pneumonia [3].…”

Section: Introductionmentioning

confidence: 99%

Horizontal Transfer Mediated Proliferation of Antibiotic Resistance Genes in High-Fat Diet Induced Obesity Mice

Wang¹,

Chen²,

Wang³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Infections caused by pathogens carrying antibiotic resistance genes (ARGs) are triggering a global health crisis. Human gut microbiota is an important reservoir of ARGs, which can not only be exchanged inside the symbiotic microbiota, but can also be horizontally transferred to opportunistic pathogens. A dysbiotic gut microbiota has been associated with obesity and other metabolic disorders, which has also been described as the most unneglected global health problem. Many studies have focused on the effects of antibiotics and other exogenous chemical on the fate of ARGs, but little is known about the effect of obesity induced dysbiotic gut microbiota on the fate of ARGs. Results This study addressed the significant proliferation of ARGs and the dramatical change of mobile genetic elements (MGEs) in high-fat diet induced obesity (DIO) mice, which the ermB and tnpA-03 genes mostly increased, demonstrating that DIO alone could enrich the abundance of ARGs. A clear increase of serine and tyrosine in the feces showed a significant positive correlation with the dominant genes of DIO groups (ermB and tnpA-03), suggesting that serine and tyrosine might enhance DNA transposition (tnpA-03) by nucleophilic attacking a phosphorus atom of a backbone phosphate group and ultimately significantly amplified the ARGs. Additionally, ermB has been frequently found in obesity-associated Lactobacillus, which might contribute to the sharp increase of ARGs and dramatical change of MGEs in the DIO mice. Finally, procrustes analysis showed the explanatory variables of the MGEs, the metabolites, and the microbial communities for the ARGs accounted for 94.3%, 53.4%, and 68.1%, respectively. Conclusion This study demonstrated that DIO significantly enhanced the abundance of ARGs and dramatically changed MGEs in mouse gut microbiota. MGEs were the most direct factor affecting ARGs, and microbiota were the main driver of the proliferation of ARGs in the DIO mice.

show abstract

Gut resistomes, microbiota and antibiotic residues in Chinese patients undergoing antibiotic administration and healthy individuals

Cited by 50 publications

References 75 publications

Fecal Microbiome and Resistome Profiling of Healthy and Diseased Pakistani Individuals Using Next-Generation Sequencing

Fecal Microbiome and Resistome Profiling of Healthy and Diseased Pakistani Individuals Using Next-Generation Sequencing

Gut Microbiota, Antibiotic Therapy and Antimicrobial Resistance: A Narrative Review

Horizontal Transfer Mediated Proliferation of Antibiotic Resistance Genes in High-Fat Diet Induced Obesity Mice

Contact Info

Product

Resources

About