Gut SCP is an immune-relevant molecule involved in the primary immunological memory or pattern recognition in the amphioxus Branchiostoma belcheri

Gut CaVP is an innate immune protein against bacterial challenge in amphioxus Branchiostoma belcheri

“…Recent reports have indicated that enhanced immune response to the following secondary bacteria and alternative complement components with specific immune priming have been characterized in the scallop Chlamys farreri and B. belcheri respectively [27,28]. Moreover, we have uncovered the ability of amphioxus sarcoplasmic calciumbinding protein (SCP) to defend against invading pathogens, and to potentially serve as a molecule involved in specific immune priming [18]. Thus, these proteins besides Ig-superfamily proteins which play a role in the primary immunological memory of invertebrates should be paid attention to.…”

“…Western blot analysis was performed as previously described [18]. Briefly, samples were resolved by SDS-PAGE on 12% gels using discontinuous buffer system and were stained with CBB.…”

Estradiol 17 beta-dehydrogenase 5 is involved in aflatoxin B1 transportation

“…After dialysis in PBS at 4 °C for 2 days and condensation with PEG 20000, the elution solution was further analyzed by LC-MS/MS. Finally, the data were analyzed with UNIPRO (22 (23). First-strand cDNA synthesis was performed using an AMV First Strand cDNA Synthesis Kit (Promega, Beijing, China).…”

Identification of AFB1-interacting proteins and interactions between RPSA and AFB1

“…Prior to be analyzed by LC-MS/MS (Thermo LTQ-20A), samples were dried by evaporation, and were re-suspended in the mixture of 3% ACN and 0.1% FA. Finally, the resulting data were analyzed by MASCOT with Uniprot/SwissProt release 7.6 as the searching database [21]. The Protein with at least 2 matched peptides and score reaching 30 were singled out and deem identified.…”

“…Polyadenylated (poly(A)) RNA was isolated from livers of healthy male Kunming mice using the TRIzol RNA extraction kit, according to the manufacturer's protocol, and held at −80 • C for further use [21]. First-strand cDNA synthesis was performed using an AMV First Strand cDNA Synthesis Kit, according to the manufacturer's protocol.…”