Guttation droplets of sugarcane red rot pathogen <i>Colletotrichum falcatum</i>: Formation, toxigenic properties, and composition

Jayakumar, V.; Senthil, Kalaiselvi

doi:10.1080/00275514.2021.1899544

“…In our investigation, two Myosin-1 proteins (A1C4A5 and Q7SDM3) were detected exclusively in C. falcatum pathogen cultured with sugarcane stalks (CFS1 and CFS2). There are some reports of toxin producing ability in some isolates of C. falcatum also (Nithya et al, 2012;Jayakumar and Senthil, 2021), which might be correlated with the enhanced expression of Myosin-1 protein in our results. String analysis of Myosin-1 protein (Q7SDM3) revealed its close interactions with the proteins involved in fungal cytoskeleton development and endocytosis like:…”

Section: Exclusively Abundant Proteins In C Falcatum In Response To R...supporting

confidence: 78%

Draft proteome of Colletotrichum falcatum revealed differential abundance of fungal pathogenicity-related proteins during red rot disease in sugarcane

Gujjar

¹

,

Joshi

²

,

Srivast

³

et al. 2023

Preprint

0

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A highly virulent Colletotrichum falcatum pathotype (CF08) was cultured in nutrient deficit media with fine-cut sugarcane stalks of red rot susceptible variety (CoJ 64). The mycelium was collected for the analysis of whole proteome using LCMS/MS. In-silico analysis was performed to identify the exclusive and differentially abundant proteins from whole proteomes of C. falcatum samples cultured with sugarcane (CFS1 and CFS2). Whole proteome analysis revealed a relatively higher number of peptide identities/proteins in CFS2 (2233), followed by CFS1 (1911), and CFC (control) with least number of proteins (1808). Compared to control, CFS1 and CFS2 samples of C. falcatum had plenty of exclusively abundant proteins like ABC transporters, glucose transporter, actin cytoskeleton-regulatory complex proteins, antimicrobial peptides, autophagy-related proteins, chitin synthase, dicer-like proteins, histone-lysine N-methyltransferase proteins, myosin-1 proteins, polyketide synthase proteins, and nonribosomal peptide synthase proteins which are involved in the process of fungal virulence/pathogenicity. In this manuscript, we report the first draft of C. falcatum proteome (whole proteome) which has been successfully submitted to ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD033368.

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“…In our investigation, two Myosin-1 proteins (A1C4A5 and Q7SDM3) were detected exclusively in C. falcatum pathogen cultured with sugarcane stalks (CFS1 and CFS2). There are some reports of toxin producing ability in some isolates of C. falcatum also (Nithya et al, 2012;Jayakumar and Senthil, 2021), which might be correlated with the enhanced expression of Myosin-1 protein in our results. String analysis of Myosin-1 protein (Q7SDM3) revealed its close interactions with the proteins involved in fungal cytoskeleton development and endocytosis like: tropomyosin, myosin tail region-interacting protein (MTI1), actin, actin-like protein (ACT2), actin polymerization protein (BZZ1), and plastin-1/ mbrin protein (Fig.…”

Section: Exclusively Abundant Proteins In C Falcatum In Response To R...supporting

confidence: 78%

Draft proteome of Colletotrichum falcatum revealed exclusive abundance of fungal pathogenicity-related proteins during red rot disease in sugarcane

Gujjar

¹

,

Kumar

²

,

Goswami

³

et al. 2023

Preprint

0

View full text Add to dashboard Cite

A highly virulent Colletotrichum falcatum pathotype (CF08) was cultured in nutrient deficit media with fine-cut sugarcane stalks of red rot susceptible variety (CoJ 64). The mycelium was collected for the analysis of whole proteome using LCMS/MS. In-silico analysis was performed to identify the exclusive and differentially abundant proteins from whole proteomes of C. falcatum samples cultured with sugarcane (CFS1 and CFS2). Whole proteome analysis revealed a relatively higher number of peptide identities/proteins in CFS2 (2233), followed by CFS1 (1911), and CFC (control) with least number of proteins (1808). Compared to control, CFS1 and CFS2 samples of C. falcatum had plenty of exclusively abundant proteins like ABC transporters, glucose transporter, actin cytoskeleton-regulatory complex proteins, antimicrobial peptides, autophagy-related proteins, chitin synthase, dicer-like proteins, histone-lysine N-methyltransferase proteins, myosin-1 proteins, polyketide synthase proteins, and nonribosomal peptide synthase proteins which are involved in the process of fungal virulence/pathogenicity. In this manuscript, we report the first draft of C. falcatum proteome (whole proteome) which has been successfully submitted to ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD033368.

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AI-Enhanced Remote Sensing Applications in Indian Sugarcane Research: A Comprehensive Review

Vinayaka,

Prasad

2024

Sugar Tech

1

0

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Guttation droplets of sugarcane red rot pathogen Colletotrichum falcatum: Formation, toxigenic properties, and composition

Cited by 3 publications

References 23 publications

Draft proteome of Colletotrichum falcatum revealed differential abundance of fungal pathogenicity-related proteins during red rot disease in sugarcane

Draft proteome of Colletotrichum falcatum revealed differential abundance of fungal pathogenicity-related proteins during red rot disease in sugarcane

Draft proteome of Colletotrichum falcatum revealed exclusive abundance of fungal pathogenicity-related proteins during red rot disease in sugarcane

AI-Enhanced Remote Sensing Applications in Indian Sugarcane Research: A Comprehensive Review

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