Gynoecium development: networks in Arabidopsis and beyond

Zúñiga-Mayo, Víctor M.; Gómez-Felipe, Andrea; Herrera-Ubaldo, Humberto; Folter, Stefan de

doi:10.1093/jxb/erz026

Cited by 47 publications

(31 citation statements)

References 148 publications

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“…Hormonal signalling is an integral part of carpel development, with crucial functions for signalling pathways such as auxin and cytokinin, but also others like brassinosteroids and gibberellins (Marsch-Martinez and de Folter, 2016;Zúñiga-Mayo et al, 2019). We observed expression of many of the genes and transcription factors related to these hormonal pathways (Fig.…”

Section: Expression Dynamics Of Carpel Developmental Regulatorsmentioning

confidence: 68%

“…While genetic and protein interactions of many of the TRs coordinating carpel development are known (reviewed in Reyes-Olalde et al, 2013, Chávez Montes et al, 2015, Zúñiga-Mayo et al, 2019, and Becker 2019, we lack a comprehensive picture of expression dynamics of these TRs during carpel development. So far, the major transcriptomic studies of flower development in A. thaliana have focused on either the later stages of the developed flower organs (Klepikova et al, 2016) or complete buds at early to late stages (Ryan et al, 2015 andMantegazza et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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High-resolution temporal transcript profiling duringArabidopsis thalianagynoecium morphogenesis uncovers the chronology of gene regulatory network activity and reveals novel developmental regulators

Kivivirta

Herbert

Roessner

et al. 2020

Preprint

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The gynoecium is the most complex organ formed by the flowering plants. It encloses the ovules, provides a surface for pollen contact and self-incompatibility reactions, allows pollen tube growth and, post fertilization, and develops into the fruit. Consequently, the regulation of gynoecium morphogenesis is complex and appropriate timing of this process in part determines reproductive success. However, little is known about the global control of gynoecium development, even though many regulatory genes have been characterized. Here, we characterized dynamic gene expression changes using laser-microdissected gynoecium tissue from four developmental stages in Arabidopsis. We provide a high-resolution map of global expression dynamics during gynoecium morphogenesis and link these to the gynoecium interactome. We reveal groups of genes acting together early and others acting late in morphogenesis. Clustering of co-expressed genes enables comparisons between the leaf, shoot apex, and gynoecium transcriptomes allowing the dissection of common and distinct regulators. Furthermore, our results lead to the discovery of the LESSER FERTILITY1-4 (LEF1-4) genes, which, when mutated, lead to impaired gynoecium expansion, illustrating that global transcriptome analyses reveal yet unknown developmental regulators. Our data show that highly interacting proteins, such as SEPALLATA3, AGAMOUS, and TOPLESS are expressed more evenly during development, but switch interactors in time, whereas stage-specific proteins have only few interactors. Our analysis connects specific transcriptional regulator activities, protein interactions, and underlying metabolic processes towards the development of a dynamic network model for gynoecium development.

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Section: Expression Dynamics Of Carpel Developmental Regulatorsmentioning

confidence: 68%

Section: Introductionmentioning

confidence: 99%

High-resolution temporal transcript profiling duringArabidopsis thalianagynoecium morphogenesis uncovers the chronology of gene regulatory network activity and reveals novel developmental regulators

Kivivirta

Herbert

Roessner

et al. 2020

Preprint

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“…CRC genes are also responsible for floral meristem termination in eudicots and monocots [33,55,56]. In the case of Arabidopsis, the role of CRC in meristem determinacy is masked by redundant pathways with AGAMOUS and only crc-1 ag-1/+ and crc-1 ap2-2 pi-1 ag-1 mutants show abnormal carpel proliferation in the center of the flower [5,9,14,33,55,56].…”

Section: Expression Of Afimcrc Is Likely Correlated With the Receptacmentioning

confidence: 99%

“…In Arabidopsis thaliana, SPT promotes the development of specialized tissues of the carpel margins, and regulates the differentiation of the style, the stigma, and the transmitting tissue [5,9,60]. Additionally, SPT specifies the differentiation of the valve margins and the dehiscence zone during carpel development and, together with INDEHISCENT (IND), it also regulates the auxin biosynthesis and distribution in the medial tissues during development [14,61,62]. Expression of SPT homologs in species of Solanaceae is broad in sepals, petals, stamens and carpels [63].…”

Section: Reduced Levels Of Afimspt In the Stigmas Are Only Found Tranmentioning

confidence: 99%

“…The gene regulatory networks involved in gynoecium patterning have been well characterized in Arabidopsis thaliana [3,[7][8][9][10][11][12][13]. These networks integrate different transcription factor families, hormones, microRNAs, peptides and chromatin-modifying proteins that ultimately define carpel identity and tissue specialization of the gynoecium from fertilization to fruit maturation [6,14]. Carpel identity is specified by C-and E-function genes, specifically by the tetramer formed between AGA-MOUS (AG) and SEPALLATA (SEP) MADS-box genes [8,[15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

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Expression of gynoecium patterning transcription factors in Aristolochia fimbriata (Aristolochiaceae) and their contribution to gynostemium development

Peréz-Mesa

Ortiz-Ramírez

González

et al. 2020

EvoDevo

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Background: In Aristolochia (Aristolochiaceae) flowers, the congenital fusion of the anthers and the commissural, stigmatic lobes forms a gynostemium. Although the molecular bases associated to the apical-basal gynoecium patterning have been described in eudicots, comparative expression studies of the style and stigma regulatory genes have never been performed in early divergent angiosperms possessing a gynostemium. Results: In this study, we assess the expression of five genes typically involved in gynoecium development in Aristolochia fimbriata. We found that all five genes (AfimCRC , AfimSPT, AfimNGA, AfimHEC1 and AfimHEC3) are expressed in the ovary, the placenta, the ovules and the transmitting tract. In addition, only AfimHEC3, AfimNGA and AfimSPT are temporarily expressed during the initiation of the stigma, while none of the genes studied is maintained during the elaboration of the stigmatic surfaces in the gynostemium. Conclusions: Expression patterns suggest that CRC , HEC, NGA and SPT homologs establish ovary and style identity in Aristolochia fimbriata. Only NGA,HEC3 and SPT genes may play a role in the early differentiation of the stigmatic lobes, but none of the genes studied seems to control late stigma differentiation in the gynostemium. The data gathered so far raises the possibility that such transient expression early on provides sufficient signal for late stigma differentiation or that unidentified late identity genes are controlling stigma development in the gynostemium. Our data does not rule out the possibility that stigmas could correspond to staminal filaments with convergent pollen-receptive surfaces.

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Carpels

Smyth¹

2022

Encyclopedia of Life Sciences

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Gynoecium development: networks in Arabidopsis and beyond

Cited by 47 publications

References 148 publications

High-resolution temporal transcript profiling duringArabidopsis thalianagynoecium morphogenesis uncovers the chronology of gene regulatory network activity and reveals novel developmental regulators

High-resolution temporal transcript profiling duringArabidopsis thalianagynoecium morphogenesis uncovers the chronology of gene regulatory network activity and reveals novel developmental regulators

Expression of gynoecium patterning transcription factors in Aristolochia fimbriata (Aristolochiaceae) and their contribution to gynostemium development

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