Gypenosides induced G0/G1 arrest via CHk2 and apoptosis through endoplasmic reticulum stress and mitochondria-dependent pathways in human tongue cancer SCC-4 cells

Chen, Jung Chou; Lu, Kaining; Tsai, Ming Li; Hsu, Shu Chun; Kuo, Chao Lin; Yang, Jai Sing; Hsia, Te Chun; Yu, Chun Shu; Chou, Su Tze; Kao, Ming‐Ching; Chung, Jing Gung; Wood, W. G.

doi:10.1016/j.oraloncology.2008.05.012

Cited by 88 publications

(91 citation statements)

References 33 publications

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“…Several drugs and plant extracts have been shown to arrest cell growth at the G0-G1 phase and induce apoptosis in different cancer cell lines. These include: Gypenosides, a component of Gynostemma pentaphyllum Makino [29], Adiponectin [30], Terfenadine, a histamine H1 receptor antagonist [31] and Aucubin, an extract from the leaves of Aucuba japonica [32]. Curcumin has been reported to induce G1/S or G2/M arrest and apoptosis in other cancer cell lines [33] suggesting that the effect of curcumin is cancer cell type dependent.…”

Section: Discussionmentioning

confidence: 99%

Synergistic apoptotic effect of Arabinoxylan rice bran (MGN-3/Biobran) and Curcumin (Turmeric) on human multiple myeloma cell line U266 in vitro

Ghoneum¹,

Gollapudi²

2011

neo

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The present study was carried out to investigate the synergistic apoptotic potential of arabinoxylan rice bran (MGN-3/Biobran) and curcumin (turmeric) on human multiple myeloma (MM) cell line U266 , in vitro. U266 cells were cultured with MGN-3 (50 or 100μg/ml) and curcumin (2.5-10μM) for 3 days. The effects of MGN-3 and curcumin on the growth and survival of the U266 cells were determined by trypan blue, MTT assay, flow cytometry analysis of cancer cell cycle, and apoptosis. Expression of proapoptotic Bax, and antiapoptotic Bcl2 was determined by Western blot analysis. Treatment with MGN-3 alone or curcumin alone caused a dose-dependent inhibition in the proliferation of U266 cells. However, a synergistic effect was noticed post-treatment with both agents that maximized at 100μg/ml MGN-3 plus 10μM curcumin. This synergy was characterized by an 87% decrease in cell number and a 2.6 fold increase in the percentage of apoptotic U266 cells. Cell cycle analysis showed a 53% decrease in the percentage of cells in the G0-G1 phase treated with MGN-3 and curcumin (from 36% to 17%). Analysis of the expression of the pro and antiapoptotic molecules Bax and Bcl-2 revealed synergistic effects of these agents, as the expression of Bcl-2 was decreased and Bax was increased. This resulted in a cellular microenvironment favorable for apoptosis. We conclude that MGN-3 and curcumin synergize in the induction of U266 cell apoptosis. This data may establish the foundation for in vivo studies that could have therapeutic implications.

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Section: Discussionmentioning

confidence: 99%

Synergistic apoptotic effect of Arabinoxylan rice bran (MGN-3/Biobran) and Curcumin (Turmeric) on human multiple myeloma cell line U266 in vitro

Ghoneum¹,

Gollapudi²

2011

neo

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“…and their polysaccharides extract did have the strong capability in improving metabolic abnormalities in in vivo studies [186][187][188][189]. Furthermore, a saponin extract from Gynostemma Pentaphyllum (GP) -gypenoside has been demonstrated to inhibit tumors through inducing mitochondria-dependent apoptosis and cell cycle arrest at G0/G1 [190]. It has been demonstrated that GP is able to modulate blood glucose levels and reduce hyperlipidemia in the Zucker fatty rat [191].…”

Section: Summary and Potential In The Application Of Herbal Researchmentioning

confidence: 99%

Glucose Metabolism in Breast Cancer and its Implication in Cancer Therapy

Li¹,

Tan²,

Li³

et al. 2011

IJCM

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“…A375.S2 cells (5x10 4 cells/well) were placed on 4-well chamber slides before being treated with 250 μM of GA for 24 h. Then cells were fixed in 3% formaldehyde in PBS for 15 min, permeabilized with 0.1% Triton X-100 in PBS for 1 h with blocking of non-specific binding sites using 2% BSA as described previously (25). Fixed cells were stained with primary antibodies to cytochrome c, GADD153 and GRP78 (1:100 dilution) overnight and then stained with secondary antibody (FITC-conjugated goat anti-mouse IgG at 1:100 dilution) (green fluorescence), followed by mitochondria and nuclei counterstaining which were individual with MitoTracker ® Red CMXRos and PI (Molecular Probes/ Invitrogen Corp.) (red fluorescence).…”

Section: Apoptotic-associated Proteins Examined By Western Blottingmentioning

confidence: 99%

“…Fixed cells were stained with primary antibodies to cytochrome c, GADD153 and GRP78 (1:100 dilution) overnight and then stained with secondary antibody (FITC-conjugated goat anti-mouse IgG at 1:100 dilution) (green fluorescence), followed by mitochondria and nuclei counterstaining which were individual with MitoTracker ® Red CMXRos and PI (Molecular Probes/ Invitrogen Corp.) (red fluorescence). Photomicrographs were obtained using a Leica TCS SP2 confocal spectral microscope (25).…”

Section: Apoptotic-associated Proteins Examined By Western Blottingmentioning

confidence: 99%

Gallic acid induces apoptosis in A375.S2 human melanoma cells through caspase-dependent and -independent pathways

Lai²,

Yang

et al. 2010

Int J Oncol

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Abstract. The natural antioxidant gallic acid (GA) has demonstrated a significant inhibition of cell proliferation and induction of apoptosis in a series of cancer cell lines. However, there is no available information to show whether GA induces apoptosis in human skin cancer cells. In the present study, we report GA-induced apoptosis in A375.S2 human melanoma cells. GA affected morphological changes, decreased the percentage of viable cells and induced apoptosis in A375.S2 cells in a dose-and time-dependent manner. Observation of the molecular mechanism of apoptosis in A375.S2 cells showed that GA up-regulated the proapoptotic proteins such as Bax, and induced caspase cascade activity, but down-regulated antiapoptotic proteins such as Bcl-2. GA induced reactive oxygen species (ROS) and intracellular Ca 2+ productions and decreased the level of mitochondrial membrane potential (Δae m ) in A375.S2 cells in a timedependent manner. GA triggered cytosolic release of apoptotic molecules, cytochrome c, promoted activation of caspase-9 and caspase-3, and ultimately apoptotic cell death. In addition, GA also promoted cytosolic release of apoptosisinducing factor (AIF) and endonuclease G (Endo G). Therefore, GA may also induce apoptosis through a caspaseindependent pathway. Our results suggest that GA might be a potential anticancer compound; however, in depth in vivo studies are needed to elucidate the exact mechanism.

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Gypenosides induced G0/G1 arrest via CHk2 and apoptosis through endoplasmic reticulum stress and mitochondria-dependent pathways in human tongue cancer SCC-4 cells

Cited by 88 publications

References 33 publications

Synergistic apoptotic effect of Arabinoxylan rice bran (MGN-3/Biobran) and Curcumin (Turmeric) on human multiple myeloma cell line U266 in vitro

Synergistic apoptotic effect of Arabinoxylan rice bran (MGN-3/Biobran) and Curcumin (Turmeric) on human multiple myeloma cell line U266 in vitro

Glucose Metabolism in Breast Cancer and its Implication in Cancer Therapy

Gallic acid induces apoptosis in A375.S2 human melanoma cells through caspase-dependent and -independent pathways

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