H-2/HLA CROSS-REACTIONS Absorption Analysis of cytotoxic Antihuman Activity in Anti-H-2 Mouse Sera

Unexpected Lympho‐cytotoxic Reactions of Anti‐h‐2 Sera on Normal Lymph‐node Cells: Are They Due to Altered H‐2 Structures or Anti‐viral Antibodies?

“…We hypothesized ) that these reactions on human cells might indicate the presence of alterations in antigen expression analogous to those responsible for the unusual cross-reactivity pattern shown by the individual H-2 serum described herein. The reactivity (with human cells) of pools of anti-H-2 sera produced in the same donor-recipient combination as the individual mouse serum described in this communication has been reported previously Ivanyi et al, 1976).…”

“…Hence, only I@ and Kq are expected to remain as negative targets (Kb and R were present in the recipients) as well as all D locus alles (except @. Previous data on anti-H-2 sera prepared as H-2 serum pools in the same combination confirmed these expectations (see Catalogue of NIH H-2 sera or Ivanyi et al, 1976). In our present experiments we did not work with H-2 serum pools, but tested the sera from individual immunized mice.…”

Cytotoxic Effect of Anti‐H‐2 and Anti‐Ia Antisera on Human B and T Cells

“…The cytotoxicity titer or cytotoxicity scoring grade of H-2 antisera correlated with the presence of certain HLA antigens on the human lymphocytes, namely A2, A9, B7, and B27. Appropriately diluted or absorbed anti-H-2 antisera had correlation coefficients as high as 0.80 with these HLA antigens (1,4,5). Absorptions of anti-H-2 antisera with mouse lymphoid cells with different H-2 haplotypes showed that the antibodies responsible for the reactivity with human cells were probably produced against public H-2 specificities (5).…”

“…Appropriately diluted or absorbed anti-H-2 antisera had correlation coefficients as high as 0.80 with these HLA antigens (1,4,5). Absorptions of anti-H-2 antisera with mouse lymphoid cells with different H-2 haplotypes showed that the antibodies responsible for the reactivity with human cells were probably produced against public H-2 specificities (5). So far the determinants on human lymphocytes responsible for the observed H-2/HLA crossreactions have not been precisely defined.…”

Anti‐H‐2Dd Antibodies Cross‐React with HLA—A11 and Aw31

“…Previous data on H-2/HLA cross-reactions have shown that certain alloimmune anti-H-2 sera cross-react with the HLA antigens A2, A9, B7 and B27. The H-2 sera which have shown these cross-reactions were generated against whole H-2 haplotype differences; more specific anti-H-2 sera prepared by mutual immunization of strains with recombinant H-2 haplotypes have until now not shown specific (HLArelated) cytotoxic reactions with human lymphocytes (Ivaskova et al 1975, 1976, Ivanyi et al 1976, 1977.…”