H-2-Specific T Suppressor Cells I. Evidence for T Suppressor Inducer and Effector Molecules in Suppression

Malley, Arthur; Zeleny-Pooley, Michelle; Kelleher-Mayo, Susan

doi:10.1097/00007890-199310000-00028

Cited by 3 publications

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“…Since footpad testing is done within 18 h of transfer, and the transfer of control cells between the two groups had no effect on the level of DH, however, it is possible that the lack o f suppression resulted from a lack of cell cooperation rather than from an alloge neic effect. The requirement for the CD4+ cell in the pathway lead ing to the development of MAN-specific CD8+ effectorsuppressor cells is consistent with studies involving (a) ani mal models and fungi other than C. albicans [14,28], (b) human peripheral blood lymphocytes and H-2-specific sup pression [29], (c) suppression of immunoglobulin synthesis by T cell lymphocytic leukemia cells [30] and (d) Concanavalin A-induced suppression of immunoglobulin synthesis by murine cells [31]. A CD4+ suppressor-inducer cell has also been described among human T cells which induced suppression of immunoglobulin production in vitro [32], as well as among human T cells responding in vitro to the solu ble antigen PPD [33], Moreover.…”

Section: Discussionsupporting

confidence: 86%

Candida albicans Mannan-Specific, Delayed Hypersensitivity Down-Regulatory CD8+ Cells Are Genetically Restricted Effectors and Their Production Requires CD4 and l-A Expression

S²,

Wang³

et al. 1996

Int Arch Allergy Immunol

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There is considerable controversy over the induction and activity of down-regulatory cells active in various antigen-specific and antigen-nonspecific systems. We have been studying the nature of such cells in a Candida albicans mannan (MAN)-specifιc system for some time and report here the requirements for CD4+ and I-A+ cells during the inductive phase for the development of CD8+ effector cells, as well as the requirement for genetic compatibility for effector activity of CD8+ cells. Since we have shown previously that CD8+ down-regulatory cells were present in spleens of MAN-treated mice 4 days following the administration of MAN to naive mice, as determined by their ability to suppress delayed hypersensitivity (DH) when transferred to immunized recipients, we treated mice with monoclonal antibodies specific for CD4 and I-A at various times before, with or after the administration of MAN to assess the role of CD4+ and I-A+ cells in the development of the CD8+ effector cell. Both anti-CD4 and anti-I-A given before or up to 30 h after the administration of MAN abrogated the ability of splenocytes from MAN-treated mice to down-regulate MAN-specific DH in immunized recipients. Moreover, transfers of down-regulatory cells between H-2-incompatible strains of mice, specifically CBA/J and BALB/cByJ, provided evidence that the effector cell for the down-regulatory activity was also restricted genetically in its activity. Taken together, the data presented indicate that genetically compatible cells are required for both the inductive and effector stages of down-regulation of MAN-specific DH, suggesting that cell-cell cooperation is required for both stages and that CD4+ cells are required in a pathway leading to the development of the CD8+ effector cell.

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Section: Discussionsupporting

confidence: 86%

Candida albicans Mannan-Specific, Delayed Hypersensitivity Down-Regulatory CD8+ Cells Are Genetically Restricted Effectors and Their Production Requires CD4 and l-A Expression

S²,

Wang³

et al. 1996

Int Arch Allergy Immunol

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Stabilization and characterization of antigen-specific T suppressor inducer and T suppressor effector molecules

Malley

Zeleny-Pooley

Murray

1995

Journal of Immunological Methods

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Extracellular (Soluble) Antigen-Specific T Cell Proteins Related to the T Cell Receptor for Antigen (sTCRr): Serologic and Primary Amino Acid Sequence Similarity to T Cell Receptor Alpha Chains and Association with Cytokines

Cone

O’Rourke²,

Malley³

1998

Journal of Interferon & Cytokine Research

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Antigen-specific-effected immunoregulation by T lymphocytes is mediated by extracellular proteins produced by T lymphocytes. These immunoproteins bind specifically to nonprocessed antigen and either induce antigen-specific immunoregulatory T cells (tsfi) or effect regulation (tsfe). T cell proteins that bind specifically to nonprocessed antigen have ben termed "T cell antigen-binding molecules" (TABM), and by definition, tsfe and tsfi are, in part, TABM. To characterize tsfi, tsfe, and TABM and understand the relationships and function of these immunoproteins, we have combined the efforts of two laboratories to compare tsfi, tsfe, and TABM isolated by each laboratory. Data obtained in one laboratory were reproduced by the other, and all reagents prepared by each laboratory were exchanged. TABM, tsfi, and tsfe were found to express TCRCalpha epitopes but not TCRCbeta epitopes. The amino acid sequence of a tryptic peptide of a T cell hybridoma TABM specific for nitrophenylhydroxy acetate (NP) is similar to a TCRalpha chain and TCR pre-alpha chain amino acid sequence. ELISA and immunoblotting demonstrated that Mr 77,000 T cell hybrid-derived tsfi, tsfe, and TABM are noncovalently associated with Mr 15,000-16,000 interleukin-10 (IL-10). ELISA also demonstrated that tsfi and tsfe are associated with I-J. The ability of tsfi and tsfe to suppress a mixed lymphocyte reaction was prevented by anti-IL-10 or anti-I-J antibodies, suggesting that antigen-specific immunoregulatory T cell proteins function by an antigen-specific focusing of immunoregulatory cytokines.

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H-2-Specific T Suppressor Cells I. Evidence for T Suppressor Inducer and Effector Molecules in Suppression

Cited by 3 publications

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<i>Candida albican</i><i>s</i> Mannan-Specific, Delayed Hypersensitivity Down-Regulatory CD8+ Cells Are Genetically Restricted Effectors and Their Production Requires CD4 and l-A Expression

<i>Candida albican</i><i>s</i> Mannan-Specific, Delayed Hypersensitivity Down-Regulatory CD8+ Cells Are Genetically Restricted Effectors and Their Production Requires CD4 and l-A Expression

Stabilization and characterization of antigen-specific T suppressor inducer and T suppressor effector molecules

Extracellular (Soluble) Antigen-Specific T Cell Proteins Related to the T Cell Receptor for Antigen (sTCRr): Serologic and Primary Amino Acid Sequence Similarity to T Cell Receptor Alpha Chains and Association with Cytokines

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