Magnetoacoustic concentration tomography with magnetic induction (MACT-MI) is a noninvasive imaging method that reconstructs the concentration image of magnetic nanoparticles (MNPs) based on the acoustic pressure signal generated by the magnetic properties of MNPs. The performance of MNPs is of great significance in MACT-MI. To study influences of the uniaxial anisotropy of MNPs on MACT-MI, firstly, based on the static magnetization curve, the force characteristic that the MNPs with uniaxial anisotropy experienced was analyzed. The magnetic force equation with the space component separated from the time term was deduced. The acoustic pressure equation containing the concentration of the MNPs with uniaxial anisotropy was derived. Then, a two-dimensional axisymmetric simulation model was constructed to compare magnetic force, acoustic source, and acoustic pressure before and after considering the uniaxial anisotropy of MNPs. The effect of scanning angle and detection radius of ultrasonic transducer on the acoustic pressure was studied. Finally, the concentration image of the MNPs with uniaxial anisotropy was reconstructed by the time reversal method and the method of moments (MoM). Theoretical considerations and simulation results have shown that the magnetic force has a triple increase after taking into account the uniaxial anisotropy of MNPs. The take-off time of acoustic pressure waves is only related to the position of the uniaxial anisotropy MNPs region. From the reconstructed image, concentration distribution and spatial location and size information of the uniaxial anisotropy MNPs region can be distinguished. The research results may lay the foundation for MACT-MI in subsequent experiments and even clinical applications.