H. Influenzae Meningitis in Relation to Treatment

Thomson, John; Bruce, Laetitia M.; Green, M. G.

doi:10.1136/bmj.2.4523.414

Cited by 11 publications

(3 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These favourable results with ampicillin stand in contrast to the reported experience with penicillin G even when patients treated with the latter drug had meningitis due to H. influenza strains sensitive to penicillin G, and C.S.F. penicillin G levels were maintained by intrathecal therapy (Drysd'ale, McIntosh and Brodie, 1946;Zinnemann, 1946;Thomson, Bruce and Green, 1947). Perhaps qualitative differences in the activity of ampicillin and penicillin G against H. influenza account for the more effective results with ampicillin.…”

Section: Discussionmentioning

confidence: 80%

H. Influenzæ Meningitis: A Controlled Study of Treatment with Ampicillin

Thrupp

Leedom

Ivler

et al. 1964

Postgraduate Medical Journal

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 80%

H. Influenzæ Meningitis: A Controlled Study of Treatment with Ampicillin

Thrupp

Leedom

Ivler

et al. 1964

Postgraduate Medical Journal

View full text Add to dashboard Cite

“…We are impressed by the similarities between capsule expression in H. influenzae as compared with other encapsulated bacterial pathogens, particularly Streptococcus pneumoniae (27 (3/15) of all systemic infections occurring during a 14-mo period, an incidence of type a infection substantially higher than expected based on extensive experiences in the USA and elsewhere (30)(31)(32). The possession by these type a isolates of an EcoRI fragment similar to that found in type b strains is provocative.…”

Section: Discussionmentioning

confidence: 99%

Cloning of chromosomal DNA from Haemophilus influenzae. Its use for studying the expression of type b capsule and virulence.

Moxon¹,

Deich²,

Connelly³

1984

J. Clin. Invest.

View full text Add to dashboard Cite

As bstract. Haemophilus influenzae may make any one of six chemically distinct capsular polysaccharides, but only strains of capsular serotype b commonly cause systemic infection (e.g., meningitis) in humans. Molecular cloning of DNA was used to investigate the expression of type b capsule and its association with H. influenzae virulence. A virulent H. influenzae type b strain was used to construct a lambda library of chromosomal DNA in Charon 4. Two independently isolated recombinant phage were isolated from the library and were found to possess DNA necessary for expression of type b capsule. Using a well-characterized rat model of H. influenzae systemic infection, we showed that type b transformants elicited by the cloned DNA were pathogenic, causing bacteremia and meningitis, whereas the untransformed capsule-deficient H. influenzae organisms were not. A 4.4-kb EcoRI fragment, common to both DNA clones, was used to characterize clinical isolates representing all six encapsulated serotypes as well as several capsule-deficient H. influenzae by Southern hybridization analysis. The probe hybridized to an identical sized (4.4 kb) fragment of EcoRI-digested chromosomal DNA from eight independently isolated type b strains. Single bands of homology to the probe were also found in EcoRI fragments ofchromosomal DNA obtained from 33 encapsulated, nontype b H. influenzae. However, the size of these EcoRI fragments proved to be characteristic for each of the different capsular serotypes. These studies A preliminary report ofthis work was presented at the Society

show abstract

“…Forgacs, Hutchinson, and Rewell (1945) recommend that influenzal meningitis should be treated with a combination of penicillin and a sulphonamide, and report two cases, of which one recovered. Thomson, Bruce, and Green (1947) put on record four cases, of which three recovered; they advocate the use of intramuscular penicillin and an oral sulphonamide along with the intrathecal administration of 50,000 units of penicillin daily until the temperature has been normal for seven days. Gottlieb and Forsyth (1947) report four cases in infants, three of whom recovered on treatment with sulphadiazine by mouth and penicillin given intramuscularly and intrathecally.…”

mentioning

confidence: 99%