H-Rubies, a new family of red emitting fluorescent pH sensors for living cells

Despras, Guillaume; Zamaleeva, Alsu I.; Dardevet, Lucie; Tisseyre, Céline; Magalhães, João G.; Garner, Charlotte; Waard, Michel De; Amigorena, Sébastian; Feltz, Anne; Mallet, Jean‐Maurice; Collot, Mayeul

doi:10.1039/c5sc01113b

Cited by 52 publications

(41 citation statements)

References 49 publications

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“…Our human body is a weak alkaline biological system in general [19,20] . For instance, the pH in blood plasma is at 7.35-7.45 [21][22][23] , in large intestine and pancreas, the pH values are above 8.0. The pH in mitochondria area is ~8.0 [24][25][26] , and the optimal pH range of alkaline phosphatase (ALP, an enzyme mainly generated by liver and widely existed in liver, bone, kidney and intestines) in neutrophils is between 8.0 and 10.0 [27,28] .…”

Section: Research Highlightmentioning

confidence: 99%

A smart near-infrared fluorescent probe for H2O2 in alkaline environment and H2O2 imaging in vitro and in vivo

2018

Macrophage

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Development of fluorescent probes for selectively detecting two or more events simultaneously is imperative for further application in bio-detecting area. Recently, an intelligent fluorescent probe based on Changsha (CS NIR) dye and arylboronic acid (CSBOH) that can selectively detect H 2 O 2 in alkaline environment was reported. The probe can detect both H 2 O 2 and pH change simultaneously with different fluorescent signal changes, which will be more useful in the H 2 O 2 detection in living organisms. CSBOH exhibited remarkably different fluorescence changes at 650 nm and 720 nm in the presence of H 2 O 2 in different pH buffers when excited at 560 and 670 nm, respectively. The cell experiments demonstrated that CSBOH can image endogenously generated H 2 O 2 in Macrophages and A431 cells. CSBOH was also used to image H 2 O 2 in living animals successfully.

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Section: Research Highlightmentioning

confidence: 99%

A smart near-infrared fluorescent probe for H2O2 in alkaline environment and H2O2 imaging in vitro and in vivo

2018

Macrophage

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“…4, 5 The key challenge in cellular imaging is to stain cell compartments with high specificity and persistence. Although numerous efficient molecular probes have been designed to selectively stain specific cellular structures including mitochondria, 6 lipid droplets 7 , endoplasmic reticulum 8 , nucleus 7 and lysosomes 10–11 ,there is still a demand for efficient and bright fluorescent PM markers. 12 Fluorescently labelled lectins notably wheat germ agglutinin (WGA) and concanavalin A 9 are popular fluorescent membrane probes.…”

Section: Introductionmentioning

confidence: 99%

MemBright: a Family of Fluorescent Membrane Probes for Advanced Cellular Imaging and Neuroscience

Collot

Ashokkumar

Anton

et al. 2018

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The proper staining of the plasma membrane (PM) is critical in bioimaging as it 16 delimits the cell. Herein, we developed MemBright: a family of six cyanine--based fluorescent 17 turn--on PM probes that emit from orange to near--infrared when reaching the PM, and 18 enable homogeneous and selective PM staining with excellent contrast in mono and two--19 photon microscopy. These probes are compatible with long--term live cell imaging and 20 immunostaining. Moreover, MemBright label neurons in a brighter manner than 21 surrounding cells allowing identification of neurons in acute brain tissue section and 22 neuromuscular--junctions without any use of transfection or transgenic animals. At last, 23MemBright were used in super--resolution imaging to unravel the dendritic spines' neck. 3D 24 multicolor dSTORM in combination with immunostaining revealed en--passant synapse 25 displaying endogenous glutamate receptors clustered at the axonal--dendritic contact site. 26MemBright probes thus constitute a universal toolkit for cell biology and neuroscience 27 biomembrane imaging with a variety of microscopy techniques. 29 30Visualizing plasma membrane is particularly important in neuroscience, because it enables 1 visualization neuronal organization and membrane trafficking involved in the synaptic 2 transmission. 3 Recent years have seen a tremendous expansion of fluorescence imaging 3 techniques and tools for cellular research. In addition to genetically encoded fluorescent 4 proteins, a number of molecular probes for monitoring cellular events have been 5 developed. 4, 5 The key challenge in cellular imaging is to stain cell compartments with high 6 specificity and persistence. Although numerous efficient molecular probes have been 7 designed to selectively stain specific cellular structures including mitochondria, 6 lipid 8 droplets, 7 endoplasmic reticulum, 8 nucleus 9 and lysosomes, 10--11 there is still a demand for 9 efficient and bright fluorescent PM markers. 12 Fluorescently labelled lectins notably wheat 10 germ agglutinin (WGA) and concanavalin A 9 are popular fluorescent membrane probes. 11Despite their efficiency and ease of use, these probes are expensive and much larger than 12 molecular probes. The small size of the latter, comparable to lipids, allows their precise 13 location in the lipid bilayer, which is of key importance for studies of the lateral lipid 14 organization of biomembranes (lipid rafts), 12, 13 FRET with membrane proteins 14 and super--15 resolution imaging. 15--16 Therefore, molecular probes are an interesting alternative to 16 protein--based membrane markers. Although highly hydrophobic fluorophores are efficient 17 markers of membrane models such as liposomes, they generally fail in staining the cell PM of 18 live cells as they tend to precipitate before reaching the membrane and to quickly cross the 19 PM to stain inner membranes. 12, 17 Recent efforts have been made to develop specific and 20 efficient PM probes for cell imaging with various fluorophores including chromone, 18--19 ...

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“…The key challenge in cellular imaging is to stain cell compartments with high specificity and persistence. Although numerous efficient molecular probes have been designed to selectively stain specific cellular structures including mitochondria (Neto et al, 2013), lipid droplets (Collot et al, 2018), endoplasmic reticulum (Arai et al, 2014), nucleus (Thermo Fisher) and lysosomes (Despras et al, 2015) (Chen et al, 2015), there is still a demand for efficient and bright fluorescent PM markers (Klymchenko and Kreder, 2014). Fluorescently labelled lectins notably wheat germ agglutinin (WGA) and concanavalin A are popular fluorescent membrane probes.…”

Section: Introductionmentioning

confidence: 99%

MemBright: A Family of Fluorescent Membrane Probes for Advanced Cellular Imaging and Neuroscience

et al. 2018

Self Cite

View full text Add to dashboard Cite

The proper staining of the plasma membrane (PM) is critical in bioimaging as it delimits the cell. Herein, we developed MemBright: a family of six cyanine-based fluorescent turn-on PM probes that emit from orange to near-infrared when reaching the PM, and enable homogeneous and selective PM staining with excellent contrast in mono and two-photon microscopy. These probes are compatible with long-term live cell imaging and immunostaining. Moreover, MemBright label neurons in a brighter manner than surrounding cells allowing identification of neurons in acute brain tissue section and neuromuscularjunctions without any use of transfection or transgenic animals. At last, MemBright were used in super-resolution imaging to unravel the dendritic spines' neck. 3D multicolor dSTORM in combination with immunostaining revealed en-passant synapse displaying endogenous glutamate receptors clustered at the axonal-dendritic contact site. MemBright probes thus constitute a universal toolkit for cell biology and neuroscience biomembrane imaging with a variety of microscopy techniques.

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H-Rubies, a new family of red emitting fluorescent pH sensors for living cells

Abstract: H-Rubies is a family of pH probes that display a bright red fluorescence upon acidification. They have been used as molecular form to monitor mitochondrial acidification and as functionalised forms to provide ratiometric systems to measure phagosomal and endosomal pH in macrophages.

Cited by 52 publications

References 49 publications

A smart near-infrared fluorescent probe for H2O2 in alkaline environment and H2O2 imaging in vitro and in vivo

A smart near-infrared fluorescent probe for H2O2 in alkaline environment and H2O2 imaging in vitro and in vivo

MemBright: a Family of Fluorescent Membrane Probes for Advanced Cellular Imaging and Neuroscience

MemBright: A Family of Fluorescent Membrane Probes for Advanced Cellular Imaging and Neuroscience

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