H₂-driven xylitol production inCupriavidus necatorH16

Abstract

Background: Biocatalysis offers a potentially greener alternative to chemical processes. For biocatalytic systems requiring cofactor recycling, hydrogen emerges as an attractive reducing agent. Hydrogen is attractive because all the electrons can be fully transferred to the product, and it can be efficiently produced from water using renewable electricity. In this article, resting cells ofCupriavidus necatorH16 harboring a NAD-dependent hydrogenase were employed for cofactor recycling to reduce D-xylose to xylitol, a commonly used sweetener. To enable this bioconversion, D-xylose reductase fromScheffersomyces stipitiswas heterologously expressed inC. necator. Results: D-xylose reductase was successfully expressed inC. necator, enabling complete bioconversion of 30 g/L of D-xylose into xylitol within 7 days using resting cells. It was found that over 90% of the energy and protons derived from hydrogen were spent for the bioconversion, demonstrating the efficiency of the system. The highest xylitol productivity reached was 0.7 g L-1h-1. Additionally, the same chassis efficiently produced L-arabitol and D-ribitol from L-arabinose and D-ribose, respectively. Conclusions: This study highlights the efficient utilization of renewable hydrogen as a reducing agent to power cofactor recycling. Hydrogen-oxidizing bacteria, such asC. necator, can be promising hosts for performing hydrogen-driven biocatalysis.